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Protein

NAD(P)H-hydrate epimerase

Gene

mug182

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX (By similarity). May have a role in meiosis.UniRule annotation1 Publication

Catalytic activityi

(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621PotassiumUniRule annotation
Metal bindingi128 – 1281PotassiumUniRule annotation
Binding sitei161 – 1611NAD(P)HXUniRule annotation
Metal bindingi164 – 1641PotassiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

ReactomeiR-SPO-197264. Nicotinamide salvaging.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-hydrate epimeraseUniRule annotation (EC:5.1.99.6)
Alternative name(s):
Meiotically up-regulated gene 182 protein
NAD(P)HX epimeraseUniRule annotation
Gene namesi
Name:mug182
ORF Names:SPAC15A10.05c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC15A10.05c.
PomBaseiSPAC15A10.05c. mug182.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • mitochondrion Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 242242NAD(P)H-hydrate epimerasePRO_0000119062Add
BLAST

Proteomic databases

MaxQBiO13725.

Interactioni

Protein-protein interaction databases

MINTiMINT-4667422.

Structurei

3D structure databases

ProteinModelPortaliO13725.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 221211YjeF N-terminalUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni61 – 655NAD(P)HXUniRule annotation
Regioni132 – 1387NAD(P)HXUniRule annotation

Sequence similaritiesi

Belongs to the NnrE/AIBP family.UniRule annotation
Contains 1 YjeF N-terminal domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000174236.
InParanoidiO13725.
KOiK17759.
OMAiHLGMFGY.
OrthoDBiEOG092C4KKU.
PhylomeDBiO13725.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase. 1 hit.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13725-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSISCLSASA AKALDAELMS AGAFSIDQLM ELAGLSVSQA VYREYPPSQY
60 70 80 90 100
SQVLICCGPG NNGGDGLVAA RHLWQYGYKP TVYYPKPSSP ELYKRLCKQL
110 120 130 140 150
DDLDIPVVKS HDSNHFSQLL RDSKLVVDSI FGFSFKGPVR DPFGSILAAI
160 170 180 190 200
VESKIKVLSV DAPSSWEIDE GPQKEGPLKD FDPDTLISLT APKPCSKFYK
210 220 230 240
GKHYLGGRFV SKVITKKFNL SLPPYPGIDQ VVDITNKPLS MV
Length:242
Mass (Da):26,466
Last modified:January 1, 1998 - v1
Checksum:i2B13C1833EFAD37D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB10102.1.
PIRiT37705.
RefSeqiNP_594292.1. NM_001019715.2.

Genome annotation databases

EnsemblFungiiSPAC15A10.05c.1; SPAC15A10.05c.1:pep; SPAC15A10.05c.
GeneIDi2542742.
KEGGispo:SPAC15A10.05c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB10102.1.
PIRiT37705.
RefSeqiNP_594292.1. NM_001019715.2.

3D structure databases

ProteinModelPortaliO13725.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4667422.

Proteomic databases

MaxQBiO13725.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC15A10.05c.1; SPAC15A10.05c.1:pep; SPAC15A10.05c.
GeneIDi2542742.
KEGGispo:SPAC15A10.05c.

Organism-specific databases

EuPathDBiFungiDB:SPAC15A10.05c.
PomBaseiSPAC15A10.05c. mug182.

Phylogenomic databases

HOGENOMiHOG000174236.
InParanoidiO13725.
KOiK17759.
OMAiHLGMFGY.
OrthoDBiEOG092C4KKU.
PhylomeDBiO13725.

Enzyme and pathway databases

ReactomeiR-SPO-197264. Nicotinamide salvaging.

Miscellaneous databases

PROiO13725.

Family and domain databases

Gene3Di3.40.50.10260. 1 hit.
HAMAPiMF_01966. NADHX_epimerase. 1 hit.
InterProiIPR004443. YjeF_N_dom.
IPR032976. YJEFN_prot_eukaryotes.
[Graphical view]
PANTHERiPTHR13232. PTHR13232. 1 hit.
PfamiPF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF64153. SSF64153. 1 hit.
TIGRFAMsiTIGR00197. yjeF_nterm. 1 hit.
PROSITEiPS51385. YJEF_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNNRE_SCHPO
AccessioniPrimary (citable) accession number: O13725
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.