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Protein

Glucan endo-1,3-alpha-glucosidase agn1

Gene

agn1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in cell separation where it is required for the degradation of the cell wall material surrounding the septum (the septum edging) which must be hydrolyzed before full separation of the daughter cells can occur. Hydrolyzes 1,3-alpha-glucan predominantly into pentasaccharides.1 Publication

Catalytic activityi

Endohydrolysis of (1->3)-alpha-D-glucosidic linkages in isolichenin, pseudonigeran and nigeran.

GO - Molecular functioni

  • glucan endo-1,3-alpha-glucosidase activity Source: PomBase

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell septum edging catabolic process Source: PomBase
  • extracellular polysaccharide metabolic process Source: PomBase
  • fungal-type cell wall disassembly involved in conjugation with cellular fusion Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell cycle, Cell division, Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH71. Glycoside Hydrolase Family 71.
mycoCLAPiMUT71A_SCHPO.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-alpha-glucosidase agn1 (EC:3.2.1.59)
Alternative name(s):
Endo-1,3-alpha-glucanase agn1
Gene namesi
Name:agn1
ORF Names:SPAC14C4.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC14C4.09.
PomBaseiSPAC14C4.09. agn1.

Subcellular locationi

GO - Cellular componenti

  • actin fusion focus Source: PomBase
  • cell wall Source: UniProtKB-SubCell
  • extracellular region Source: PomBase
  • mating projection tip Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020CuratedAdd
BLAST
Chaini21 – 424404Glucan endo-1,3-alpha-glucosidase agn1PRO_0000012111Add
BLAST

Post-translational modificationi

Not glycosylated.

Interactioni

Subunit structurei

Monomer.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 71 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000158769.
InParanoidiO13716.
OMAiHTGWLDM.
OrthoDBiEOG7966R1.
PhylomeDBiO13716.

Family and domain databases

InterProiIPR005197. Glyco_hydro_71.
[Graphical view]
PfamiPF03659. Glyco_hydro_71. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O13716-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLVLFLVLL FSALINLTNA DKMVVAHFIV GNTYPYTVSN WEEDIQDAIA
60 70 80 90 100
VGIDGFALNM GSDAWQVERI EDAYDAAASV SSDFKLFISF DMSIISADAD
110 120 130 140 150
FIEGVVRRFA DKPNQLYYDG KVFVSTFAGE TDTFGYSDVS TGWDSAVKEP
160 170 180 190 200
LASAGYPIYF VPSWTSLGQG ALEESVADGF LSWNAWPTTD ADMNDNDDIG
210 220 230 240 250
YQNLANSLGK LYVAPVSPWF YTHLSYKNWA YKSDWLIIDR WNEMLSVQPD
260 270 280 290 300
MIEVLTWNDY GESHYIGNIQ GALPAGSEGY VDGFDHTAWR YLMSPYISAY
310 320 330 340 350
KLGLSEPYIN FESLFYWYRP TPKSATATAD SLSYPSGGDY MEDEIFVLVY
360 370 380 390 400
LLQSAEVTVT CGSTTQTFSG VPGVNQFTIP METNASPSFT VARQGGTLAS
410 420
GTGPEIVDSL SIYNFNAYTG VLYF
Length:424
Mass (Da):46,974
Last modified:August 16, 2004 - v2
Checksum:i2D680FDB8C56AEBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY626901 mRNA. Translation: AAT84064.1.
CU329670 Genomic DNA. No translation available.
AB027828 Genomic DNA. Translation: BAA87132.1.
PIRiT37694.

Genome annotation databases

EnsemblFungiiSPAC14C4.09.1; SPAC14C4.09.1:pep; SPAC14C4.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY626901 mRNA. Translation: AAT84064.1.
CU329670 Genomic DNA. No translation available.
AB027828 Genomic DNA. Translation: BAA87132.1.
PIRiT37694.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH71. Glycoside Hydrolase Family 71.
mycoCLAPiMUT71A_SCHPO.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC14C4.09.1; SPAC14C4.09.1:pep; SPAC14C4.09.

Organism-specific databases

EuPathDBiFungiDB:SPAC14C4.09.
PomBaseiSPAC14C4.09. agn1.

Phylogenomic databases

HOGENOMiHOG000158769.
InParanoidiO13716.
OMAiHTGWLDM.
OrthoDBiEOG7966R1.
PhylomeDBiO13716.

Miscellaneous databases

NextBioi20811601.
PROiO13716.

Family and domain databases

InterProiIPR005197. Glyco_hydro_71.
[Graphical view]
PfamiPF03659. Glyco_hydro_71. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Role of the alpha-glucanase Agn1p in fission-yeast cell separation."
    Dekker N., Speijer D., Grun C.H., Van Den Berg M., De Haan A., Hochstenbach F.
    Mol. Biol. Cell 15:3903-3914(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Large-scale screening of intracellular protein localization in living fission yeast cells by the use of a GFP-fusion genomic DNA library."
    Ding D.-Q., Tomita Y., Yamamoto A., Chikashige Y., Haraguchi T., Hiraoka Y.
    Genes Cells 5:169-190(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 23-62.
    Strain: ATCC 38364 / 968.

Entry informationi

Entry nameiAGN1_SCHPO
AccessioniPrimary (citable) accession number: O13716
Secondary accession number(s): Q6F6J1, Q9USE2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 16, 2004
Last modified: February 17, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.