Reviewed, UniProtKB/Swiss-Prot O13685 (UBC13_SCHPO)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 13
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase 13
    Ubiquitin carrier protein 13

Gene names

Name:	ubc13
Synonyms:	spu13
ORF Names:	SPAC11E3.04c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Has a role in the DNA error-free postreplication repair (PRR) pathway. The ubc13/spm2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. Ref.1
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Heterodimer with spm2.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW postreplication repair Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe protein ubiquitination Inferred from direct assay. Source: GeneDB_SPombe regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein homodimerization activity Inferred from direct assay. Source: GeneDB_SPombe ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	Ubiquitin-conjugating enzyme E2 13		PRO_0000082564
Sites
	Active site	86	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O13685-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 4BD0FAC35A571B9C	FASTA	148	16,856
	10 20 30 40 50 60 MALPKRIIKE IETLTRDPPP GIVAAPTEDN LRYFKITMEG PQQSAYEGGK FHLELFLPDE 70 80 90 100 110 120 YPMMPPNVRF LTKIYHPNVD KLGRICLSTL KKDWSPALQI RTVLLSIQAL MGAPNPDDPL 130 140 DNDVAKIWKE NEPQAIANAR EWTKKYAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural and functional conservation of error-free DNA postreplication repair in Schizosaccharomyces pombe." Brown M., Zhu Y., Hemmingsen S.M., Xiao W. DNA Repair 1:869-880(2002) [PubMed: 12531016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SPM2.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF470232 mRNA. Translation: AAL79844.1. CU329670 Genomic DNA. Translation: CAB11183.1.
PIR	T37532.
RefSeq	NP_594929.1.
3D structure databases
HSSP	HSSP built from PDB template 1JAT based on UniProtKB P52490.
SMR	O13685. Positions 2-147.
ModBase	Search...
Genome annotation databases
GeneID	2542925.
GenomeReviews	Gene locus ubc13 in contig CU329670_GR.
KEGG	spo:SPAC11E3.04c.
NMPDR	fig|4896.1.peg.4899.
Organism-specific databases
GeneDB_Spombe	SPAC11E3.04c.
Phylogenomic databases
OMA	LGAPNPD.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002991-MON.
BRENDA	6.3.2.19. 653.
Gene expression databases
ArrayExpress	O13685.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC13_SCHPO

Accession

Primary (citable) accession number: O13685

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)