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Protein

Ubiquitin-conjugating enzyme E2 13

Gene

ubc13

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in the DNA error-free postreplication repair (PRR) pathway. The ubc13/spm2 heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'.1 Publication

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathway: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei86 – 861Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • postreplication repair Source: PomBase
  • protein K63-linked ubiquitination Source: PomBase
  • protein ubiquitination Source: PomBase
  • UV-damage excision repair Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 13 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 13
Ubiquitin-protein ligase 13
Gene namesi
Name:ubc13
Synonyms:spu13
ORF Names:SPAC11E3.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC11E3.04c.
PomBaseiSPAC11E3.04c. ubc13.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Ubiquitin-conjugating enzyme E2 13PRO_0000082564Add
BLAST

Proteomic databases

MaxQBiO13685.

Interactioni

Subunit structurei

Heterodimer with spm2.

Protein-protein interaction databases

BioGridi279366. 40 interactions.
MINTiMINT-4667157.
STRINGi4896.SPAC11E3.04c.1.

Structurei

3D structure databases

ProteinModelPortaliO13685.
SMRiO13685. Positions 2-147.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiO13685.
KOiK10580.
OMAiYFDVEIH.
OrthoDBiEOG7SBP18.
PhylomeDBiO13685.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPKRIIKE IETLTRDPPP GIVAAPTEDN LRYFKITMEG PQQSAYEGGK
60 70 80 90 100
FHLELFLPDE YPMMPPNVRF LTKIYHPNVD KLGRICLSTL KKDWSPALQI
110 120 130 140
RTVLLSIQAL MGAPNPDDPL DNDVAKIWKE NEPQAIANAR EWTKKYAV
Length:148
Mass (Da):16,856
Last modified:January 1, 1998 - v1
Checksum:i4BD0FAC35A571B9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF470232 mRNA. Translation: AAL79844.1.
CU329670 Genomic DNA. Translation: CAB11183.1.
PIRiT37532.
RefSeqiNP_594929.1. NM_001020360.2.

Genome annotation databases

EnsemblFungiiSPAC11E3.04c.1; SPAC11E3.04c.1:pep; SPAC11E3.04c.
GeneIDi2542925.
KEGGispo:SPAC11E3.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF470232 mRNA. Translation: AAL79844.1.
CU329670 Genomic DNA. Translation: CAB11183.1.
PIRiT37532.
RefSeqiNP_594929.1. NM_001020360.2.

3D structure databases

ProteinModelPortaliO13685.
SMRiO13685. Positions 2-147.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279366. 40 interactions.
MINTiMINT-4667157.
STRINGi4896.SPAC11E3.04c.1.

Proteomic databases

MaxQBiO13685.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC11E3.04c.1; SPAC11E3.04c.1:pep; SPAC11E3.04c.
GeneIDi2542925.
KEGGispo:SPAC11E3.04c.

Organism-specific databases

EuPathDBiFungiDB:SPAC11E3.04c.
PomBaseiSPAC11E3.04c. ubc13.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
InParanoidiO13685.
KOiK10580.
OMAiYFDVEIH.
OrthoDBiEOG7SBP18.
PhylomeDBiO13685.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi20803961.
PROiO13685.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural and functional conservation of error-free DNA postreplication repair in Schizosaccharomyces pombe."
    Brown M., Zhu Y., Hemmingsen S.M., Xiao W.
    DNA Repair 1:869-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH SPM2.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiUBC13_SCHPO
AccessioniPrimary (citable) accession number: O13685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: January 1, 1998
Last modified: June 24, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.