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O13651 (SYIC_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase, cytoplasmic

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:irs1
ORF Names:pi058, SPBC8D2.06
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length1064 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003

Subcellular location

Cytoplasm Ref.3.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10641064Isoleucine--tRNA ligase, cytoplasmic HAMAP-Rule MF_02003
PRO_0000098603

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_02003
Motif597 – 6015"KMSKS" region HAMAP-Rule MF_02003

Sites

Binding site6001ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
O13651 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 94AEBA7378BEB665

FASTA1,064122,916
        10         20         30         40         50         60 
MSFNVPKEEE KIVEFWREID AFHTQLKLSQ GRPTYTFFDG PPFATGRPHH GHLLASTIKD 

        70         80         90        100        110        120 
SVTRYACLKG YHVERRFGWD THGLPVEHEI DKKLGITGSD DVMAMGIDKY NAECRKIVMT 

       130        140        150        160        170        180 
YASEWRATVE RLGRWIDFDN DYKTLYPSFM ESVWWVFKEL HTKGKVYRGY RVMPYSTACT 

       190        200        210        220        230        240 
TPLSNFEAQQ NYKEVPDPAI VVAFQSISDP EVSFLAWTTT PWTLPSNLAL AVHPDLQYIK 

       250        260        270        280        290        300 
ILDKDSNKKY ILMESCLGIL YKNPKKANFE ILERFQGKAL DGQKYEPLFP YFKSTFGERA 

       310        320        330        340        350        360 
FKLYSADYVE EGSGTGIVHQ APAFGEADYD AAWAAGIIDA DHQPPCPVDE QGLLTSEITD 

       370        380        390        400        410        420 
FAGQYVKDAD KEIIRSLKAS GHLVKHSQIF HSYPFCWRSD TPLIYRAVPS WFVRVKEITN 

       430        440        450        460        470        480 
EMVENVMSTH WVPQNIRDKR FANWLKNARD WNISRNRYWG TPIPLWVSDD YEEVVCIGSI 

       490        500        510        520        530        540 
KELEELSGVS NITDIHRDSI DHITIPSKKG KGTLHRVSEV FDCWFESGSM PYASRHYPFE 

       550        560        570        580        590        600 
RIEEFKHGFP ADFISEGVDQ TRGWFYTLTV LGTLLFDKAP YKNVIVSGLV MAEDGKKMSK 

       610        620        630        640        650        660 
RLKNYPEPNL IIEKYGSDAL RLYLINSPVV RAEILKFKED GVREVVTRVL IPWWNSYKFF 

       670        680        690        700        710        720 
EAQAALYKKV TGKDFVFDDA ATLSSNVMDR WILARCQSLI GFVDEEMKQY RLYTVVPQLL 

       730        740        750        760        770        780 
GLIEEMTNWY IRFNRRRLKG EDGEIETINA LNVLFEVLFT LVRIMGPFTP FITENIYQHL 

       790        800        810        820        830        840 
RNYMPIDKNE ISLRSVHFLP FPTYKSELDD ETVLRRVKRM QTIIELARYV REQNNISLKT 

       850        860        870        880        890        900 
PLKTLIVILT NEEYLEDAKL LERYIAEELN VREVVFTSNE EKYGVVYSVQ ADWPVLGKKL 

       910        920        930        940        950        960 
RKDMARVKKA LPNVTSEEVK EFQKNKKMVL DGIELVEGDL QIIRSVEVKN EFLKSNTDGI 

       970        980        990       1000       1010       1020 
CIVLLDIEID AQLQAEGLAR EVINRVQRLR KKSNLQVTDD VRMTYKIKND TIGLESAVDS 

      1030       1040       1050       1060 
NEALFSKVLR RPIEKETGAD ESNIIASEEQ DVQGATFLLS LLRL 

« Hide

References

[1]"A 38 kb segment containing the cdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment."
Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K., Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y., Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.
Yeast 16:71-80(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[3]"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB004538 Genomic DNA. Translation: BAA21439.1.
CU329671 Genomic DNA. Translation: CAA17821.1.
PIRT40751.
RefSeqNP_595569.1. NM_001021464.2.

3D structure databases

ProteinModelPortalO13651.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid277759. 4 interactions.
DIPDIP-59122N.
MINTMINT-4666965.
STRING4896.SPBC8D2.06-1.

Proteomic databases

MaxQBO13651.
PaxDbO13651.
PRIDEO13651.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPBC8D2.06.1; SPBC8D2.06.1:pep; SPBC8D2.06.
GeneID2541245.
KEGGspo:SPBC8D2.06.

Organism-specific databases

PomBaseSPBC8D2.06.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246403.
KOK01870.
OMAENRDWFE.
OrthoDBEOG7FNCGS.
PhylomeDBO13651.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02003. Ile_tRNA_synth_type2.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
ProtoNetSearch...

Other

NextBio20802357.
PROO13651.

Entry information

Entry nameSYIC_SCHPO
AccessionPrimary (citable) accession number: O13651
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries