O13651 (SYIC_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase, cytoplasmic
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	irs1
ORF Names:	pi058, SPBC8D2.06

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	1064 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Cytoplasm Ref.3.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Non-traceable author statement. Source: PomBase isoleucyl-tRNA aminoacylation Inferred by curator. Source: PomBase regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytosol Inferred from direct assay Ref.3. Source: PomBase
Molecular_function	ATP binding Inferred from sequence model. Source: PomBase aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from sequence or structural similarity. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1064	1064	Isoleucine--tRNA ligase, cytoplasmic		PRO_0000098603
Regions
	Motif	42 – 52	11	"HIGH" region
	Motif	597 – 601	5	"KMSKS" region
Sites
	Binding site	600	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	O13651 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 94AEBA7378BEB665	FASTA	1,064	122,916
	10 20 30 40 50 60 MSFNVPKEEE KIVEFWREID AFHTQLKLSQ GRPTYTFFDG PPFATGRPHH GHLLASTIKD 70 80 90 100 110 120 SVTRYACLKG YHVERRFGWD THGLPVEHEI DKKLGITGSD DVMAMGIDKY NAECRKIVMT 130 140 150 160 170 180 YASEWRATVE RLGRWIDFDN DYKTLYPSFM ESVWWVFKEL HTKGKVYRGY RVMPYSTACT 190 200 210 220 230 240 TPLSNFEAQQ NYKEVPDPAI VVAFQSISDP EVSFLAWTTT PWTLPSNLAL AVHPDLQYIK 250 260 270 280 290 300 ILDKDSNKKY ILMESCLGIL YKNPKKANFE ILERFQGKAL DGQKYEPLFP YFKSTFGERA 310 320 330 340 350 360 FKLYSADYVE EGSGTGIVHQ APAFGEADYD AAWAAGIIDA DHQPPCPVDE QGLLTSEITD 370 380 390 400 410 420 FAGQYVKDAD KEIIRSLKAS GHLVKHSQIF HSYPFCWRSD TPLIYRAVPS WFVRVKEITN 430 440 450 460 470 480 EMVENVMSTH WVPQNIRDKR FANWLKNARD WNISRNRYWG TPIPLWVSDD YEEVVCIGSI 490 500 510 520 530 540 KELEELSGVS NITDIHRDSI DHITIPSKKG KGTLHRVSEV FDCWFESGSM PYASRHYPFE 550 560 570 580 590 600 RIEEFKHGFP ADFISEGVDQ TRGWFYTLTV LGTLLFDKAP YKNVIVSGLV MAEDGKKMSK 610 620 630 640 650 660 RLKNYPEPNL IIEKYGSDAL RLYLINSPVV RAEILKFKED GVREVVTRVL IPWWNSYKFF 670 680 690 700 710 720 EAQAALYKKV TGKDFVFDDA ATLSSNVMDR WILARCQSLI GFVDEEMKQY RLYTVVPQLL 730 740 750 760 770 780 GLIEEMTNWY IRFNRRRLKG EDGEIETINA LNVLFEVLFT LVRIMGPFTP FITENIYQHL 790 800 810 820 830 840 RNYMPIDKNE ISLRSVHFLP FPTYKSELDD ETVLRRVKRM QTIIELARYV REQNNISLKT 850 860 870 880 890 900 PLKTLIVILT NEEYLEDAKL LERYIAEELN VREVVFTSNE EKYGVVYSVQ ADWPVLGKKL 910 920 930 940 950 960 RKDMARVKKA LPNVTSEEVK EFQKNKKMVL DGIELVEGDL QIIRSVEVKN EFLKSNTDGI 970 980 990 1000 1010 1020 CIVLLDIEID AQLQAEGLAR EVINRVQRLR KKSNLQVTDD VRMTYKIKND TIGLESAVDS 1030 1040 1050 1060 NEALFSKVLR RPIEKETGAD ESNIIASEEQ DVQGATFLLS LLRL

References

[1]	"A 38 kb segment containing the cdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment." Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K., Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y., Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M. Yeast 16:71-80(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB004538 Genomic DNA. Translation: BAA21439.1. CU329671 Genomic DNA. Translation: CAA17821.1.
PIR	T40751.
RefSeq	NP_595569.1. NM_001021464.2.
3D structure databases
ProteinModelPortal	O13651.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-59122N.
STRING	4896.SPBC8D2.06-1.
Proteomic databases
PaxDb	O13651.
PRIDE	O13651.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC8D2.06.1; SPBC8D2.06.1:pep; SPBC8D2.06.
GeneID	2541245.
KEGG	spo:SPBC8D2.06.
Organism-specific databases
PomBase	SPBC8D2.06.
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HOG000246403.
KO	K01870.
OMA	SRSRYWG.
OrthoDB	EOG4G1QQG.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
InterPro	IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023586. Ile-tRNA-ligase_type2. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	20802357.

Entry information

Entry name

SYIC_SCHPO

Accession

Primary (citable) accession number: O13651

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program