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O13651

- SYIC_SCHPO

UniProt

O13651 - SYIC_SCHPO

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Protein

Isoleucine--tRNA ligase, cytoplasmic

Gene

irs1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei600 – 6001ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: PomBase

GO - Biological processi

  1. cytoplasmic translation Source: PomBase
  2. isoleucyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:irs1
ORF Names:pi058, SPBC8D2.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC8D2.06.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosol Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10641064Isoleucine--tRNA ligase, cytoplasmicPRO_0000098603Add
BLAST

Proteomic databases

MaxQBiO13651.
PaxDbiO13651.
PRIDEiO13651.

Interactioni

Protein-protein interaction databases

BioGridi277759. 4 interactions.
DIPiDIP-59122N.
MINTiMINT-4666965.
STRINGi4896.SPBC8D2.06-1.

Structurei

3D structure databases

ProteinModelPortaliO13651.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi597 – 6015"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
InParanoidiO13651.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG7FNCGS.
PhylomeDBiO13651.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

O13651-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFNVPKEEE KIVEFWREID AFHTQLKLSQ GRPTYTFFDG PPFATGRPHH
60 70 80 90 100
GHLLASTIKD SVTRYACLKG YHVERRFGWD THGLPVEHEI DKKLGITGSD
110 120 130 140 150
DVMAMGIDKY NAECRKIVMT YASEWRATVE RLGRWIDFDN DYKTLYPSFM
160 170 180 190 200
ESVWWVFKEL HTKGKVYRGY RVMPYSTACT TPLSNFEAQQ NYKEVPDPAI
210 220 230 240 250
VVAFQSISDP EVSFLAWTTT PWTLPSNLAL AVHPDLQYIK ILDKDSNKKY
260 270 280 290 300
ILMESCLGIL YKNPKKANFE ILERFQGKAL DGQKYEPLFP YFKSTFGERA
310 320 330 340 350
FKLYSADYVE EGSGTGIVHQ APAFGEADYD AAWAAGIIDA DHQPPCPVDE
360 370 380 390 400
QGLLTSEITD FAGQYVKDAD KEIIRSLKAS GHLVKHSQIF HSYPFCWRSD
410 420 430 440 450
TPLIYRAVPS WFVRVKEITN EMVENVMSTH WVPQNIRDKR FANWLKNARD
460 470 480 490 500
WNISRNRYWG TPIPLWVSDD YEEVVCIGSI KELEELSGVS NITDIHRDSI
510 520 530 540 550
DHITIPSKKG KGTLHRVSEV FDCWFESGSM PYASRHYPFE RIEEFKHGFP
560 570 580 590 600
ADFISEGVDQ TRGWFYTLTV LGTLLFDKAP YKNVIVSGLV MAEDGKKMSK
610 620 630 640 650
RLKNYPEPNL IIEKYGSDAL RLYLINSPVV RAEILKFKED GVREVVTRVL
660 670 680 690 700
IPWWNSYKFF EAQAALYKKV TGKDFVFDDA ATLSSNVMDR WILARCQSLI
710 720 730 740 750
GFVDEEMKQY RLYTVVPQLL GLIEEMTNWY IRFNRRRLKG EDGEIETINA
760 770 780 790 800
LNVLFEVLFT LVRIMGPFTP FITENIYQHL RNYMPIDKNE ISLRSVHFLP
810 820 830 840 850
FPTYKSELDD ETVLRRVKRM QTIIELARYV REQNNISLKT PLKTLIVILT
860 870 880 890 900
NEEYLEDAKL LERYIAEELN VREVVFTSNE EKYGVVYSVQ ADWPVLGKKL
910 920 930 940 950
RKDMARVKKA LPNVTSEEVK EFQKNKKMVL DGIELVEGDL QIIRSVEVKN
960 970 980 990 1000
EFLKSNTDGI CIVLLDIEID AQLQAEGLAR EVINRVQRLR KKSNLQVTDD
1010 1020 1030 1040 1050
VRMTYKIKND TIGLESAVDS NEALFSKVLR RPIEKETGAD ESNIIASEEQ
1060
DVQGATFLLS LLRL
Length:1,064
Mass (Da):122,916
Last modified:January 1, 1998 - v1
Checksum:i94AEBA7378BEB665
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004538 Genomic DNA. Translation: BAA21439.1.
CU329671 Genomic DNA. Translation: CAA17821.1.
PIRiT40751.
RefSeqiNP_595569.1. NM_001021464.2.

Genome annotation databases

EnsemblFungiiSPBC8D2.06.1; SPBC8D2.06.1:pep; SPBC8D2.06.
GeneIDi2541245.
KEGGispo:SPBC8D2.06.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004538 Genomic DNA. Translation: BAA21439.1 .
CU329671 Genomic DNA. Translation: CAA17821.1 .
PIRi T40751.
RefSeqi NP_595569.1. NM_001021464.2.

3D structure databases

ProteinModelPortali O13651.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 277759. 4 interactions.
DIPi DIP-59122N.
MINTi MINT-4666965.
STRINGi 4896.SPBC8D2.06-1.

Proteomic databases

MaxQBi O13651.
PaxDbi O13651.
PRIDEi O13651.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC8D2.06.1 ; SPBC8D2.06.1:pep ; SPBC8D2.06 .
GeneIDi 2541245.
KEGGi spo:SPBC8D2.06.

Organism-specific databases

PomBasei SPBC8D2.06.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
InParanoidi O13651.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG7FNCGS.
PhylomeDBi O13651.

Miscellaneous databases

NextBioi 20802357.
PROi O13651.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A 38 kb segment containing the cdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment."
    Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K., Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y., Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.
    Yeast 16:71-80(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSYIC_SCHPO
AccessioniPrimary (citable) accession number: O13651
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3