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O13651

- SYIC_SCHPO

UniProt

O13651 - SYIC_SCHPO

Protein

Isoleucine--tRNA ligase, cytoplasmic

Gene

irs1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei600 – 6001ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. isoleucine-tRNA ligase activity Source: PomBase

    GO - Biological processi

    1. cytoplasmic translation Source: PomBase
    2. isoleucyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase, cytoplasmic (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    Gene namesi
    Name:irs1
    ORF Names:pi058, SPBC8D2.06
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC8D2.06.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosol Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10641064Isoleucine--tRNA ligase, cytoplasmicPRO_0000098603Add
    BLAST

    Proteomic databases

    MaxQBiO13651.
    PaxDbiO13651.
    PRIDEiO13651.

    Interactioni

    Protein-protein interaction databases

    BioGridi277759. 4 interactions.
    DIPiDIP-59122N.
    MINTiMINT-4666965.
    STRINGi4896.SPBC8D2.06-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO13651.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi42 – 5211"HIGH" regionAdd
    BLAST
    Motifi597 – 6015"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG7FNCGS.
    PhylomeDBiO13651.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O13651-1 [UniParc]FASTAAdd to Basket

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    MSFNVPKEEE KIVEFWREID AFHTQLKLSQ GRPTYTFFDG PPFATGRPHH     50
    GHLLASTIKD SVTRYACLKG YHVERRFGWD THGLPVEHEI DKKLGITGSD 100
    DVMAMGIDKY NAECRKIVMT YASEWRATVE RLGRWIDFDN DYKTLYPSFM 150
    ESVWWVFKEL HTKGKVYRGY RVMPYSTACT TPLSNFEAQQ NYKEVPDPAI 200
    VVAFQSISDP EVSFLAWTTT PWTLPSNLAL AVHPDLQYIK ILDKDSNKKY 250
    ILMESCLGIL YKNPKKANFE ILERFQGKAL DGQKYEPLFP YFKSTFGERA 300
    FKLYSADYVE EGSGTGIVHQ APAFGEADYD AAWAAGIIDA DHQPPCPVDE 350
    QGLLTSEITD FAGQYVKDAD KEIIRSLKAS GHLVKHSQIF HSYPFCWRSD 400
    TPLIYRAVPS WFVRVKEITN EMVENVMSTH WVPQNIRDKR FANWLKNARD 450
    WNISRNRYWG TPIPLWVSDD YEEVVCIGSI KELEELSGVS NITDIHRDSI 500
    DHITIPSKKG KGTLHRVSEV FDCWFESGSM PYASRHYPFE RIEEFKHGFP 550
    ADFISEGVDQ TRGWFYTLTV LGTLLFDKAP YKNVIVSGLV MAEDGKKMSK 600
    RLKNYPEPNL IIEKYGSDAL RLYLINSPVV RAEILKFKED GVREVVTRVL 650
    IPWWNSYKFF EAQAALYKKV TGKDFVFDDA ATLSSNVMDR WILARCQSLI 700
    GFVDEEMKQY RLYTVVPQLL GLIEEMTNWY IRFNRRRLKG EDGEIETINA 750
    LNVLFEVLFT LVRIMGPFTP FITENIYQHL RNYMPIDKNE ISLRSVHFLP 800
    FPTYKSELDD ETVLRRVKRM QTIIELARYV REQNNISLKT PLKTLIVILT 850
    NEEYLEDAKL LERYIAEELN VREVVFTSNE EKYGVVYSVQ ADWPVLGKKL 900
    RKDMARVKKA LPNVTSEEVK EFQKNKKMVL DGIELVEGDL QIIRSVEVKN 950
    EFLKSNTDGI CIVLLDIEID AQLQAEGLAR EVINRVQRLR KKSNLQVTDD 1000
    VRMTYKIKND TIGLESAVDS NEALFSKVLR RPIEKETGAD ESNIIASEEQ 1050
    DVQGATFLLS LLRL 1064
    Length:1,064
    Mass (Da):122,916
    Last modified:January 1, 1998 - v1
    Checksum:i94AEBA7378BEB665
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB004538 Genomic DNA. Translation: BAA21439.1.
    CU329671 Genomic DNA. Translation: CAA17821.1.
    PIRiT40751.
    RefSeqiNP_595569.1. NM_001021464.2.

    Genome annotation databases

    EnsemblFungiiSPBC8D2.06.1; SPBC8D2.06.1:pep; SPBC8D2.06.
    GeneIDi2541245.
    KEGGispo:SPBC8D2.06.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB004538 Genomic DNA. Translation: BAA21439.1 .
    CU329671 Genomic DNA. Translation: CAA17821.1 .
    PIRi T40751.
    RefSeqi NP_595569.1. NM_001021464.2.

    3D structure databases

    ProteinModelPortali O13651.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 277759. 4 interactions.
    DIPi DIP-59122N.
    MINTi MINT-4666965.
    STRINGi 4896.SPBC8D2.06-1.

    Proteomic databases

    MaxQBi O13651.
    PaxDbi O13651.
    PRIDEi O13651.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC8D2.06.1 ; SPBC8D2.06.1:pep ; SPBC8D2.06 .
    GeneIDi 2541245.
    KEGGi spo:SPBC8D2.06.

    Organism-specific databases

    PomBasei SPBC8D2.06.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG7FNCGS.
    PhylomeDBi O13651.

    Miscellaneous databases

    NextBioi 20802357.
    PROi O13651.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A 38 kb segment containing the cdc2 gene from the left arm of fission yeast chromosome II: sequence analysis and characterization of the genomic DNA and cDNAs encoded on the segment."
      Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K., Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y., Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.
      Yeast 16:71-80(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSYIC_SCHPO
    AccessioniPrimary (citable) accession number: O13651
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3