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Protein

ATP-dependent RNA helicase mss116, mitochondrial

Gene

mss116

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

ATP-dependent RNA helicase required for mitochondrial splicing of group I and II introns. Also required for efficient mitochondrial translation (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi83 – 908ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing, Translation regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase mss116, mitochondrial (EC:3.6.4.13)
Gene namesi
Name:mss116
ORF Names:SPACTOKYO_453.03c, SPBC691.04
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC691.04.
PomBaseiSPBC691.04. mss116.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: PomBase
  • mitochondrion Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionSequence analysisAdd
BLAST
Chaini28 – 535508ATP-dependent RNA helicase mss116, mitochondrialPRO_0000256013Add
BLAST

Proteomic databases

MaxQBiO13622.

Interactioni

Protein-protein interaction databases

BioGridi277651. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliO13622.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 248179Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini262 – 429168Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi38 – 6629Q motifAdd
BLAST
Motifi195 – 1984DEAD box

Domaini

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiO13622.
KOiK17679.
OMAiKNIHESA.
OrthoDBiEOG092C1VLP.
PhylomeDBiO13622.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O13622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNISLKGAIF STVGRFNLPK VQGFIRWNST MKSQQPTLFS EVASLSSTFK
60 70 80 90 100
NSLSRAGFEK MTPVQQRVLN EVFPNEENAV VQAKTGTGKT LAFLLVAFKD
110 120 130 140 150
VLKGKPRLNS SKIHSVILSP TRELALQIFE EARKLTYGTG IRVSYAIGGN
160 170 180 190 200
SKMREENAIR RGNANLLIAT PGRLEDHLQN PRILESLSTD SFILDEADRL
210 220 230 240 250
MDMGFAESIL NIHEAVTTTK TRKLCFSATM PPKVSNVFRG ILGTDFKLIN
260 270 280 290 300
CLDPNEPPTH ERVPQFVIET KLDKVFSSSL SLLQQLTSSN PSSRIIVFLP
310 320 330 340 350
TISMVDFVGG VLENHLKIPC FILHSGLTTA QRRSITESFR KCQSGILFAT
360 370 380 390 400
DVVARGMDFP NITQVVQITG PSNTDDYIHR IGRTGRAGKT GEAYLILLEQ
410 420 430 440 450
EKPFLNSIKH LPLKRATIEP LSDQALSTLR SDIEKSSKFS RTNALKTLYG
460 470 480 490 500
SKPHVFSGSS QRRATGKNIH ESAIALFSLH DVPGLMEELI YKSRGRGSPK
510 520 530
GFRGSQLKYP SSSSSSFQRR PRSLPSRGRY QQSRR
Length:535
Mass (Da):59,449
Last modified:January 1, 1998 - v1
Checksum:i8FC4025C7EAAC0DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004535 Genomic DNA. Translation: BAA21410.1.
CU329671 Genomic DNA. Translation: CAC37365.1.
RefSeqiNP_595596.1. NM_001021492.2.

Genome annotation databases

EnsemblFungiiSPBC691.04.1; SPBC691.04.1:pep; SPBC691.04.
GeneIDi2541136.
KEGGispo:SPBC691.04.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004535 Genomic DNA. Translation: BAA21410.1.
CU329671 Genomic DNA. Translation: CAC37365.1.
RefSeqiNP_595596.1. NM_001021492.2.

3D structure databases

ProteinModelPortaliO13622.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277651. 4 interactions.

Proteomic databases

MaxQBiO13622.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC691.04.1; SPBC691.04.1:pep; SPBC691.04.
GeneIDi2541136.
KEGGispo:SPBC691.04.

Organism-specific databases

EuPathDBiFungiDB:SPBC691.04.
PomBaseiSPBC691.04. mss116.

Phylogenomic databases

InParanoidiO13622.
KOiK17679.
OMAiKNIHESA.
OrthoDBiEOG092C1VLP.
PhylomeDBiO13622.

Miscellaneous databases

PROiO13622.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMS116_SCHPO
AccessioniPrimary (citable) accession number: O13622
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.