ID   ERG24_SEPLY             Reviewed;         512 AA.
AC   O13597;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-MAR-2009, entry version 42.
DE   RecName: Full=Delta(14)-sterol reductase;
DE            EC=1.3.1.70;
DE   AltName: Full=C-14 sterol reductase;
DE   AltName: Full=Sterol C14-reductase;
GN   Name=ERG3;
OS   Septoria lycopersici (Tomato leaf spot fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Dothideomycetes; Dothideomycetidae; Capnodiales; Mycosphaerellaceae;
OC   mitosporic Mycosphaerellaceae; Septoria.
OX   NCBI_TaxID=39703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Aparna K.;
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Reduces the C14=C15 double bond of 4,4-dimethyl-
CC       cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-
CC       dienol (By similarity).
CC   -!- CATALYTIC ACTIVITY: 4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-
CC       beta-ol + NADP(+) = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-
CC       beta-ol + NADPH.
CC   -!- PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol
CC       from lanosterol: step 2/6.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
CC       (Probable).
CC   -!- SIMILARITY: Belongs to the ERG4/ERG24 family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; Y14389; CAA74747.1; -; Genomic_DNA.
DR   BRENDA; 1.3.1.70; 298049.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0050613; F:delta14-sterol reductase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001171; Ergosterol_biosynth_ERG4_ERG24.
DR   InterPro; IPR018083; Sterol_reductase_CS.
DR   Pfam; PF01222; ERG4_ERG24; 1.
DR   PROSITE; PS01017; STEROL_REDUCT_1; 1.
DR   PROSITE; PS01018; STEROL_REDUCT_2; 1.
PE   3: Inferred from homology;
KW   Lipid synthesis; Membrane; NADP; Oxidoreductase; Steroid biosynthesis;
KW   Sterol biosynthesis; Transmembrane.
FT   CHAIN         1    512       Delta(14)-sterol reductase.
FT                                /FTId=PRO_0000207498.
FT   TRANSMEM     27     47       Potential.
FT   TRANSMEM    100    120       Potential.
FT   TRANSMEM    140    160       Potential.
FT   TRANSMEM    172    192       Potential.
FT   TRANSMEM    242    262       Potential.
FT   TRANSMEM    278    298       Potential.
FT   TRANSMEM    324    344       Potential.
FT   TRANSMEM    353    373       Potential.
FT   TRANSMEM    418    438       Potential.
FT   TRANSMEM    458    478       Potential.
SQ   SEQUENCE   512 AA;  57578 MW;  169A756E60C0E963 CRC64;
     MARTKATARK TVPAQQEAHG YEFGGPIGAS LISFGLPIAC YAFGFLCNDV SGCPPPSLLS
     PSKLFTPPTL SNKVPWQHAL DTLAAEVGWP GWSGLINTEA VLGVFFWYGL SLLLWVLLPA
     HEVEGTELRT GGRLKYRFNA CLSAVTIFVA CAAGTIVRGP DFQVWTFINR NYIQLLTVNI
     IIAYALAIYV YLKSFEVKAG NTEQRELAAG GHSGHILYDW YMGRELNPRI TIPFIGEVDI
     KSFMELRPGM IGWVLLDLAF AAKQYKSYGY ITDSMRKWTP LLLGIHVLTI HPVIVIISQS
     VYVFDALYME PAILTTMDLT TDGFGFMLSF GDLVWVPFIY SIQAKYLSVH PVALGPLYVA
     LILTIQATGY YIFRATNNDK NIFRTNPNDP KVAHLKYIET GTGSRLLTTG WWGTARHINY
     LGDWLMSWSY CLPTLAAGYK LTPTILFENS RLVSTDGMKG AGIPITYFYM LYFAILLIHR
     ERRDEAKCRR KYGAHWEKYC QIVRWRILPG VY
//