Reviewed, UniProtKB/Swiss-Prot O13597 (ERG24_SEPLY)

Names and origin

Protein names

Recommended name:
    Delta(14)-sterol reductase
    EC=1.3.1.70
Alternative name(s):
    C-14 sterol reductase
    Sterol C14-reductase

Gene names

Name:

ERG3

Organism

Septoria lycopersici (Tomato leaf spot fungus)

Taxonomic identifier

39703 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Dothideomycetidae › Capnodiales › Mycosphaerellaceae › mitosporic Mycosphaerellaceae › Septoria

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol By similarity.
Catalytic activity	4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP+ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	delta14-sterol reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Delta(14)-sterol reductase		PRO_0000207498
Regions
	Transmembrane	27 – 47	21	Potential
	Transmembrane	100 – 120	21	Potential
	Transmembrane	140 – 160	21	Potential
	Transmembrane	172 – 192	21	Potential
	Transmembrane	242 – 262	21	Potential
	Transmembrane	278 – 298	21	Potential
	Transmembrane	324 – 344	21	Potential
	Transmembrane	353 – 373	21	Potential
	Transmembrane	418 – 438	21	Potential
	Transmembrane	458 – 478	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	O13597-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 169A756E60C0E963	FASTA	512	57,578
	10 20 30 40 50 60 MARTKATARK TVPAQQEAHG YEFGGPIGAS LISFGLPIAC YAFGFLCNDV SGCPPPSLLS 70 80 90 100 110 120 PSKLFTPPTL SNKVPWQHAL DTLAAEVGWP GWSGLINTEA VLGVFFWYGL SLLLWVLLPA 130 140 150 160 170 180 HEVEGTELRT GGRLKYRFNA CLSAVTIFVA CAAGTIVRGP DFQVWTFINR NYIQLLTVNI 190 200 210 220 230 240 IIAYALAIYV YLKSFEVKAG NTEQRELAAG GHSGHILYDW YMGRELNPRI TIPFIGEVDI 250 260 270 280 290 300 KSFMELRPGM IGWVLLDLAF AAKQYKSYGY ITDSMRKWTP LLLGIHVLTI HPVIVIISQS 310 320 330 340 350 360 VYVFDALYME PAILTTMDLT TDGFGFMLSF GDLVWVPFIY SIQAKYLSVH PVALGPLYVA 370 380 390 400 410 420 LILTIQATGY YIFRATNNDK NIFRTNPNDP KVAHLKYIET GTGSRLLTTG WWGTARHINY 430 440 450 460 470 480 LGDWLMSWSY CLPTLAAGYK LTPTILFENS RLVSTDGMKG AGIPITYFYM LYFAILLIHR 490 500 510 ERRDEAKCRR KYGAHWEKYC QIVRWRILPG VY

References

[1]	Aparna K. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y14389 Genomic DNA. Translation: CAA74747.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.3.1.70. 298049.
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG24_SEPLY

Accession

Primary (citable) accession number: O13597

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	March 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)