O13559 (YRF14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Y' element ATP-dependent helicase protein 1 copy 4
EC=3.6.4.12

Gene names

Name:	YRF1-4
Ordered Locus Names:	YLR466W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1382 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes DNA unwinding and is involved in telomerase-independent telomere maintenance. Ref.3
Catalytic activity	ATP + H2O = ADP + phosphate.
Induction	Induced in absence of telomerase TLC1. Ref.3
Sequence similarities	Belongs to the helicase family. Yeast subtelomeric Y' repeat subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	telomere maintenance via recombination Inferred from genetic interaction Ref.3. Source: SGD
Cellular component	nucleus Inferred by curator Ref.3. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro DNA helicase activity Inferred from direct assay Ref.3. Source: SGD nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1382	1382	Y' element ATP-dependent helicase protein 1 copy 4		PRO_0000102204
Regions
	Domain	383 – 560	178	Helicase ATP-binding
	Domain	617 – 766	150	Helicase C-terminal
	Nucleotide binding	396 – 403	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	O13559 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 47C392C1444B7AB8	FASTA	1,382	156,367
	10 20 30 40 50 60 MWKTLGRVEQ LLPYASLILR NREVLFREPK RGIDEYLEND SFFQMIPVKY REIVLPKLRR 70 80 90 100 110 120 DTNKMTAALK NKVAVAIDEL TVPLMWMIHF AVGYPYRYPE LQLLAFAGPQ RNVYVDDTTR 130 140 150 160 170 180 RIQLYTDYNK NGSSEPRLKT LDGLTSDYVF YFVTVLRQMQ ICALGNSYDA FNHDPWMDVV 190 200 210 220 230 240 GFEDPDQVTN RDISRIVLYS YMFLNTAKGC LVEYATFRQY MRELPKNAPQ KLNFREMRQG 250 260 270 280 290 300 LIALGRHCVG SRFETDLYES ATSELMANHS VQTGRNIYGV DSFSLTSVSG TTATLLQERA 310 320 330 340 350 360 SERWIQWLGL ESDYHCSFSS TRNAEDVVAG EAASSDHHQK ISRVTRKRPR EPKSTNDILV 370 380 390 400 410 420 AGRKLFGSSF EFRDLHQLRL CHEIYMADTP SVAVQAPPGY GKTELFHLPL IALASKGDVK 430 440 450 460 470 480 YVSFLFVPYT VLLANCMIRL SRCGCLNVAP VRNFIEEGCD GVTDLYVGIY DDLASTNFTD 490 500 510 520 530 540 RIAAWENIVE CTFRTNNVKL GYLIVDEFHN FETEVYRQSQ FGGITNLDFD AFEKAIFLSG 550 560 570 580 590 600 TAPEAVADAA LQRIGLTGLA KKSMDINELK RSEDLSRGLS SYPTRMFNLI KEKSEVPLGH 610 620 630 640 650 660 VHKIWKKVES QPEEALKLLL ALFEIEPESK AIVVASTTNE VEELACSWRK YFRVVWIHGK 670 680 690 700 710 720 LGAAEKVSRT KEFVTDGSMR VLIGTKLVTE GIDIKQLMMV IMLDNRLNII ELIQGVGRLR 730 740 750 760 770 780 DGGLCYLLSR KNSWAARNRK GELPPIKEGC ITEQVREFYG LESKKGKKGQ HVGCCGSRTD 790 800 810 820 830 840 LSADTVELIE RMDRLAEKQA TASMSIIALP SSFQESNSSD RCRKYCSSDE DSDTCIHGSA 850 860 870 880 890 900 NASTNATTNS STNATTTAST NVRTSATTTA SINVRTSAIT TESTNSSTNA TTTASTNVRT 910 920 930 940 950 960 SATTTASINV RTSATTTEST NSNTSATTTE STDSNTSATT TESTDSNTSA TTTASTNSST 970 980 990 1000 1010 1020 NATTTASTNS STNATTTEST NASAKEDANK DGNAEDNRFH PVTDINKESY KRKGSQMVLL 1030 1040 1050 1060 1070 1080 ERKKLKAQFP NTSENMNVLQ FLGFRSDEIK HLFLYGIDVY FCPEGVFTQY GLCKGCQKMF 1090 1100 1110 1120 1130 1140 ELCVCWAGQK VSYRRMAWEA LAVERMLRND EEYKEYLEDI EPYHGDPVGY LKYFSVKRGE 1150 1160 1170 1180 1190 1200 IYSQIQRNYA WYLAITRRRE TISVLDSTRG KQGSQVFRMS GRQIKELYYK VWSNLRESKT 1210 1220 1230 1240 1250 1260 EVLQYFLNWD EKKCREEWEA KDDTVFVEAL EKVGVFQRLR SMTSAGLQGP QYVKLQFSRH 1270 1280 1290 1300 1310 1320 HRQLRSRYEL SLGMHLRDQL ALGVTPSKVP HWTAFLSMLI GLFYNKTFRQ KLEYLLEQIS 1330 1340 1350 1360 1370 1380 EVWLLPHWLD LANVEVLAAD NTRVPLYMLM VAVHKELDSD DVPDGRFDII LLCRDSSREV GE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII." Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D. Nature 387:87-90(1997) [PubMed: 9169871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Y'-Help1, a DNA helicase encoded by the yeast subtelomeric Y' element, is induced in survivors defective for telomerase." Yamada M., Hayatsu N., Matsuura A., Ishikawa F. J. Biol. Chem. 273:33360-33366(1998) [PubMed: 9837911] [Abstract] Cited for: FUNCTION, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22383 Genomic DNA. Translation: AAB64729.1. BK006945 Genomic DNA. Translation: DAA09763.1.
PIR	S70310.
RefSeq	NP_013571.1. NM_001182354.1.
3D structure databases
ProteinModelPortal	O13559.
SMR	O13559. Positions 358-511, 614-733.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8268N.
IntAct	O13559. 9 interactions.
MINT	MINT-395409.
STRING	O13559.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YLR466W; YLR466W; YLR466W.
GeneID	851187.
KEGG	sce:YLR466W.
Organism-specific databases
CYGD	YLR466w.
SGD	S000004458. YRF1-4.
Phylogenomic databases
eggNOG	fuNOG09714.
HOGENOM	HBG204022.
OrthoDB	EOG4V9Z0T.
Gene expression databases
Genevestigator	O13559.
GermOnline	YLR466W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR001650. Helicase_C. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968022.

Entry information

Entry name

YRF14_YEAST

Accession

Primary (citable) accession number: O13559
Secondary accession number(s): D6VZ97

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	January 1, 1998
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program