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O13516

- RS9A_YEAST

UniProt

O13516 - RS9A_YEAST

Protein

40S ribosomal protein S9-A

Gene

RPS9A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.1 Publication

    GO - Molecular functioni

    1. rRNA binding Source: SGD
    2. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Biological processi

    Ribosome biogenesis, rRNA processing

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33987-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S9-A
    Alternative name(s):
    RP21
    S13
    YP28
    YS11
    Gene namesi
    Name:RPS9A
    Synonyms:RPS13A, YS11A
    Ordered Locus Names:YPL081W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    SGDiS000006002. RPS9A.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD
    2. nucleolus Source: UniProtKB-SubCell
    3. small-subunit processome Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 19719640S ribosomal protein S9-APRO_0000132703Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei184 – 1841PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO13516.
    PeptideAtlasiO13516.
    PRIDEiO13516.

    Expressioni

    Gene expression databases

    GenevestigatoriO13516.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3.2 Publications

    Protein-protein interaction databases

    BioGridi36100. 60 interactions.
    IntActiO13516. 27 interactions.
    MINTiMINT-2786178.
    STRINGi4932.YPL081W.

    Structurei

    Secondary structure

    1
    197
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi16 – 183
    Helixi21 – 3414
    Helixi39 – 6022
    Helixi67 – 8317
    Helixi88 – 903
    Helixi94 – 985
    Helixi101 – 1055
    Helixi109 – 1157
    Helixi122 – 1309
    Beta strandi135 – 1384
    Helixi153 – 1553
    Beta strandi160 – 1678
    Helixi171 – 18313

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K5Xmodel-D19-196[»]
    1S1Helectron microscopy11.70D19-197[»]
    1VW9electron microscopy6.10K1-197[»]
    1VWVelectron microscopy6.10K1-197[»]
    3IZBelectron microscopy-C1-197[»]
    3O2ZX-ray4.00E1-197[»]
    3O30X-ray4.00E1-197[»]
    3U5CX-ray3.00J1-197[»]
    3U5GX-ray3.00J1-197[»]
    4BYLelectron microscopy4.30J1-197[»]
    4BYTelectron microscopy6.60J1-197[»]
    4CUYelectron microscopy3.70J2-180[»]
    ProteinModelPortaliO13516.
    SMRiO13516. Positions 2-186.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO13516.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini107 – 18175S4 RNA-bindingPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ribosomal protein S4P family.Curated
    Contains 1 S4 RNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    GeneTreeiENSGT00550000074829.
    HOGENOMiHOG000194525.
    KOiK02997.
    OMAiQDFMERR.
    OrthoDBiEOG790GCC.

    Family and domain databases

    Gene3Di3.10.290.10. 1 hit.
    InterProiIPR022801. Ribosomal_S4/S9.
    IPR005710. Ribosomal_S4/S9_euk/arc.
    IPR001912. Ribosomal_S4/S9_N.
    IPR018079. Ribosomal_S4_CS.
    IPR002942. S4_RNA-bd.
    [Graphical view]
    PANTHERiPTHR11831. PTHR11831. 1 hit.
    PfamiPF00163. Ribosomal_S4. 1 hit.
    PF01479. S4. 1 hit.
    [Graphical view]
    SMARTiSM00363. S4. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01018. rpsD_arch. 1 hit.
    PROSITEiPS00632. RIBOSOMAL_S4. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O13516-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPRAPRTYSK TYSTPKRPYE SSRLDAELKL AGEFGLKNKK EIYRISFQLS    50
    KIRRAARDLL TRDEKDPKRL FEGNALIRRL VRVGVLSEDK KKLDYVLALK 100
    VEDFLERRLQ TQVYKLGLAK SVHHARVLIT QRHIAVGKQI VNIPSFMVRL 150
    DSEKHIDFAP TSPFGGARPG RVARRNAARK AEASGEAADE ADEADEE 197
    Length:197
    Mass (Da):22,443
    Last modified:January 23, 2007 - v3
    Checksum:iA5A3641D4D7A34F5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 223ESS → QSB AA sequence (PubMed:6814480)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00724 Genomic DNA. Translation: BAA00626.1.
    U41849 Genomic DNA. Translation: AAB68268.1.
    BK006949 Genomic DNA. Translation: DAA11352.1.
    PIRiS16822.
    RefSeqiNP_015244.1. NM_001183895.1.

    Genome annotation databases

    EnsemblFungiiYPL081W; YPL081W; YPL081W.
    GeneIDi856024.
    KEGGisce:YPL081W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00724 Genomic DNA. Translation: BAA00626.1 .
    U41849 Genomic DNA. Translation: AAB68268.1 .
    BK006949 Genomic DNA. Translation: DAA11352.1 .
    PIRi S16822.
    RefSeqi NP_015244.1. NM_001183895.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K5X model - D 19-196 [» ]
    1S1H electron microscopy 11.70 D 19-197 [» ]
    1VW9 electron microscopy 6.10 K 1-197 [» ]
    1VWV electron microscopy 6.10 K 1-197 [» ]
    3IZB electron microscopy - C 1-197 [» ]
    3O2Z X-ray 4.00 E 1-197 [» ]
    3O30 X-ray 4.00 E 1-197 [» ]
    3U5C X-ray 3.00 J 1-197 [» ]
    3U5G X-ray 3.00 J 1-197 [» ]
    4BYL electron microscopy 4.30 J 1-197 [» ]
    4BYT electron microscopy 6.60 J 1-197 [» ]
    4CUY electron microscopy 3.70 J 2-180 [» ]
    ProteinModelPortali O13516.
    SMRi O13516. Positions 2-186.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36100. 60 interactions.
    IntActi O13516. 27 interactions.
    MINTi MINT-2786178.
    STRINGi 4932.YPL081W.

    Proteomic databases

    MaxQBi O13516.
    PeptideAtlasi O13516.
    PRIDEi O13516.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPL081W ; YPL081W ; YPL081W .
    GeneIDi 856024.
    KEGGi sce:YPL081W.

    Organism-specific databases

    SGDi S000006002. RPS9A.

    Phylogenomic databases

    GeneTreei ENSGT00550000074829.
    HOGENOMi HOG000194525.
    KOi K02997.
    OMAi QDFMERR.
    OrthoDBi EOG790GCC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33987-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    EvolutionaryTracei O13516.
    NextBioi 980938.

    Gene expression databases

    Genevestigatori O13516.

    Family and domain databases

    Gene3Di 3.10.290.10. 1 hit.
    InterProi IPR022801. Ribosomal_S4/S9.
    IPR005710. Ribosomal_S4/S9_euk/arc.
    IPR001912. Ribosomal_S4/S9_N.
    IPR018079. Ribosomal_S4_CS.
    IPR002942. S4_RNA-bd.
    [Graphical view ]
    PANTHERi PTHR11831. PTHR11831. 1 hit.
    Pfami PF00163. Ribosomal_S4. 1 hit.
    PF01479. S4. 1 hit.
    [Graphical view ]
    SMARTi SM00363. S4. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01018. rpsD_arch. 1 hit.
    PROSITEi PS00632. RIBOSOMAL_S4. 1 hit.
    PS50889. S4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast ribosomal proteins: XII. YS11 of Saccharomyces cerevisiae is a homologue to E. coli S4 according to the gene analysis."
      Mizuta K., Hashimoto T., Suzuki K., Otaka E.
      Nucleic Acids Res. 19:2603-2608(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast."
      Otaka E., Higo K., Osawa S.
      Biochemistry 21:4545-4550(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-24.
    5. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "The small-subunit processome is a ribosome assembly intermediate."
      Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J.
      Eukaryot. Cell 3:1619-1626(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.
    9. "Structure of the 80S ribosome from Saccharomyces cerevisiae -- tRNA-ribosome and subunit-subunit interactions."
      Spahn C.M.T., Beckmann R., Eswar N., Penczek P.A., Sali A., Blobel G., Frank J.
      Cell 107:373-386(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 19-196, ELECTRON MICROSCOPY.
    10. "Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation."
      Spahn C.M.T., Gomez-Lorenzo M.G., Grassucci R.A., Joergensen R., Andersen G.R., Beckmann R., Penczek P.A., Ballesta J.P.G., Frank J.
      EMBO J. 23:1008-1019(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 19-197, ELECTRON MICROSCOPY.

    Entry informationi

    Entry nameiRS9A_YEAST
    AccessioniPrimary (citable) accession number: O13516
    Secondary accession number(s): D6W3T6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 131 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 106000 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for S9 in yeast.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3