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Protein

Urease

Gene

URE1

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.

Cofactori

Ni cationBy similarityNote: Binds 2 nickel ions per subunit.By similarity

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).
Proteins known to be involved in this subpathway in this organism are:
  1. Urease (URE1)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi400Nickel 1; via tele nitrogenBy similarity1
Metal bindingi402Nickel 1; via tele nitrogenBy similarity1
Metal bindingi483Nickel 1; via carbamate groupBy similarity1
Metal bindingi483Nickel 2; via carbamate groupBy similarity1
Binding sitei485SubstrateBy similarity1
Metal bindingi512Nickel 2; via pros nitrogenBy similarity1
Metal bindingi538Nickel 2; via tele nitrogenBy similarity1
Active sitei586Proton donorBy similarity1
Metal bindingi626Nickel 1By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370

Names & Taxonomyi

Protein namesi
Recommended name:
Urease (EC:3.5.1.5)
Alternative name(s):
Urea amidohydrolase
Gene namesi
Name:URE1
ORF Names:CNAG_05540
OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Taxonomic identifieri235443 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesCryptococcaceaeCryptococcusCryptococcus neoformans species complex
Proteomesi
  • UP000010091 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiFungiDB:CNAG_05540

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000675281 – 833UreaseAdd BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei483N6-carboxylysineBy similarity1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.By similarity

Interactioni

Subunit structurei

Homohexamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO13465
SMRiO13465
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini395 – 833UreaseAdd BLAST439

Sequence similaritiesi

In the C-terminal section; belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.Curated

Phylogenomic databases

OrthoDBiEOG092C0Z46

Family and domain databases

CDDicd00375 Urease_alpha, 1 hit
cd00407 Urease_beta, 1 hit
cd00390 Urease_gamma, 1 hit
Gene3Di2.10.150.10, 1 hit
2.30.40.10, 2 hits
3.30.280.10, 1 hit
HAMAPiMF_01953 Urease_alpha, 1 hit
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
IPR008221 Urease
IPR011612 Urease_alpha_N_dom
IPR017950 Urease_AS
IPR005848 Urease_asu
IPR017951 Urease_asu_c
IPR002019 Urease_beta
IPR036461 Urease_betasu_sf
IPR002026 Urease_gamma/gamma-beta_su
IPR036463 Urease_gamma_sf
IPR029754 Urease_Ni-bd
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
PF00449 Urease_alpha, 1 hit
PF00699 Urease_beta, 1 hit
PF00547 Urease_gamma, 1 hit
PIRSFiPIRSF001222 Urease, 1 hit
PRINTSiPR01752 UREASE
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002319 Urease_gamma_reg, 1 hit
SUPFAMiSSF51278 SSF51278, 1 hit
SSF51338 SSF51338, 1 hit
SSF51556 SSF51556, 2 hits
SSF54111 SSF54111, 1 hit
TIGRFAMsiTIGR01792 urease_alph, 1 hit
TIGR00192 urease_beta, 1 hit
TIGR00193 urease_gam, 1 hit
PROSITEiView protein in PROSITE
PS01120 UREASE_1, 1 hit
PS00145 UREASE_2, 1 hit
PS51368 UREASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

O13465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLLPRETDK LILTTLGTLA QRRLARGLIL NRAETIALIS SQLQEFVRDG
60 70 80 90 100
RHSVAELMDL GKKMLGRRHV RKGVPESIHT IQVEGTFPDG VFLVTVDDPI
110 120 130 140 150
SSDDGDLNNA FYGSFLPIPS ADVFPAAPEP ADTLLGALIC RKETVKINAS
160 170 180 190 200
RRRFRLEVKN AGDRPVQVGS HYHFLETNPA LIFDRLLSYG YHLDIPAGTA
210 220 230 240 250
VRFEPGEKKT VTMVEFGGKK IFHGGSGLGN GSFDENLRET KVKEMVEKGG
260 270 280 290 300
FGHKEQEKIE EGPVTEMNRE VYASMFGPTT GDKIKLADMD LWIEVEKDYT
310 320 330 340 350
VYGDECKFGG GKVIRDGGGQ ASGRHDHEVL DLVITNALIV DWTGIYKADI
360 370 380 390 400
GVKNGIIVGI GKAGNPDMMD GVTDGMIVGS STEVISGEKL ITTAGALDVH
410 420 430 440 450
VHYISPQLMT EALASGITTV IGGGTGPADG SNATTCTSSS FYMQNMIKAT
460 470 480 490 500
DTIPLNFGFT GKGSDSGTNA MRDIIEAGAC GLKVHEDWGA TPEVIDRALS
510 520 530 540 550
MADEYDVQIN LHSDTLNESG YVESTLAAIK GRTIHSYHTE GAGGGHAPDI
560 570 580 590 600
IVVCEYENVL PSSTNPTRPY AVNTLDEHLD MLMICHGLDK SIPEDIAFAD
610 620 630 640 650
SRIRSETVAA EDVLQDTGAI SMISSDCQAM GRIGEVVTRT WRTAAKMKQF
660 670 680 690 700
RGPLEGDEPT RDNNRVKRYV AKYTINPAIT HGMSHLIGQV AVGCLADLVF
710 720 730 740 750
WTAESFGARP EMILKGGVIA WAAVGDANAS IPTVQPVLGR PMWGSQPEAA
760 770 780 790 800
ALNSIVWVSQ ASLDKDLVKR FNIKKRAEAV KNCRSIGKKD MKWNDTMPKM
810 820 830
TVDPETYDVR ADGVLCDVPP ADKLPLTRRY FVY
Length:833
Mass (Da):90,475
Last modified:February 6, 2013 - v3
Checksum:i89E4D04C82FFE5ED
GO

Sequence cautioni

The sequence AFR98966 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti249G → V in AAC62257 (PubMed:10639402).Curated1
Sequence conflicti396A → R in AAC62257 (PubMed:10639402).Curated1
Sequence conflicti492P → A in AAC62257 (PubMed:10639402).Curated1
Sequence conflicti700 – 703FWTA → LLDG in AAC62257 (PubMed:10639402).Curated4
Sequence conflicti744 – 757GSQPE…NSIVW → ALSLRPLHSIQLF in AAC62257 (PubMed:10639402).CuratedAdd BLAST14
Sequence conflicti771 – 774FNIK → YRLR in AAC62257 (PubMed:10639402).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006062 Genomic DNA Translation: AAC62257.1
CP003833 Genomic DNA Translation: AFR98966.2 Sequence problems.
RefSeqiXP_012053644.1, XM_012198254.1

Genome annotation databases

EnsemblFungiiAFR98966; AFR98966; CNAG_05540
GeneIDi23888844

Similar proteinsi

Entry informationi

Entry nameiUREA_CRYNH
AccessioniPrimary (citable) accession number: O13465
Secondary accession number(s): J9VZV0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: February 6, 2013
Last modified: March 28, 2018
This is version 103 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health