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Protein

Urease

Gene

URE1

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.

Cofactori

Ni cationBy similarityNote: Binds 2 nickel ions per subunit.By similarity

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).
Proteins known to be involved in this subpathway in this organism are:
  1. Urease (URE1)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi400 – 4001Nickel 1; via tele nitrogenBy similarity
Metal bindingi402 – 4021Nickel 1; via tele nitrogenBy similarity
Metal bindingi483 – 4831Nickel 1; via carbamate groupBy similarity
Metal bindingi483 – 4831Nickel 2; via carbamate groupBy similarity
Binding sitei485 – 4851SubstrateBy similarity
Metal bindingi512 – 5121Nickel 2; via pros nitrogenBy similarity
Metal bindingi538 – 5381Nickel 2; via tele nitrogenBy similarity
Active sitei586 – 5861Proton donorBy similarity
Metal bindingi626 – 6261Nickel 1By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease (EC:3.5.1.5)
Alternative name(s):
Urea amidohydrolase
Gene namesi
Name:URE1
ORF Names:CNAG_05540
OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Taxonomic identifieri235443 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
Proteomesi
  • UP000010091 Componenti: Chromosome 14

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 833833UreasePRO_0000067528Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei483 – 4831N6-carboxylysineBy similarity

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.By similarity

Interactioni

Subunit structurei

Homohexamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO13465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini395 – 833439UreaseAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.Curated
Contains 1 urease domain.Curated

Phylogenomic databases

OrthoDBiEOG092C0Z46.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
cd00407. Urease_beta. 1 hit.
cd00390. Urease_gamma. 1 hit.
Gene3Di2.10.150.10. 1 hit.
2.30.40.10. 1 hit.
3.30.280.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR008221. Urease.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR002019. Urease_beta.
IPR002026. Urease_gamma/gamma-beta_su.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
PF00699. Urease_beta. 1 hit.
PF00547. Urease_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF001222. Urease. 1 hit.
PRINTSiPR01752. UREASE.
ProDomiPD002319. Urease_gamma_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51278. SSF51278. 1 hit.
SSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 2 hits.
SSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
TIGR00192. urease_beta. 1 hit.
TIGR00193. urease_gam. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13465-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLLPRETDK LILTTLGTLA QRRLARGLIL NRAETIALIS SQLQEFVRDG
60 70 80 90 100
RHSVAELMDL GKKMLGRRHV RKGVPESIHT IQVEGTFPDG VFLVTVDDPI
110 120 130 140 150
SSDDGDLNNA FYGSFLPIPS ADVFPAAPEP ADTLLGALIC RKETVKINAS
160 170 180 190 200
RRRFRLEVKN AGDRPVQVGS HYHFLETNPA LIFDRLLSYG YHLDIPAGTA
210 220 230 240 250
VRFEPGEKKT VTMVEFGGKK IFHGGSGLGN GSFDENLRET KVKEMVEKGG
260 270 280 290 300
FGHKEQEKIE EGPVTEMNRE VYASMFGPTT GDKIKLADMD LWIEVEKDYT
310 320 330 340 350
VYGDECKFGG GKVIRDGGGQ ASGRHDHEVL DLVITNALIV DWTGIYKADI
360 370 380 390 400
GVKNGIIVGI GKAGNPDMMD GVTDGMIVGS STEVISGEKL ITTAGALDVH
410 420 430 440 450
VHYISPQLMT EALASGITTV IGGGTGPADG SNATTCTSSS FYMQNMIKAT
460 470 480 490 500
DTIPLNFGFT GKGSDSGTNA MRDIIEAGAC GLKVHEDWGA TPEVIDRALS
510 520 530 540 550
MADEYDVQIN LHSDTLNESG YVESTLAAIK GRTIHSYHTE GAGGGHAPDI
560 570 580 590 600
IVVCEYENVL PSSTNPTRPY AVNTLDEHLD MLMICHGLDK SIPEDIAFAD
610 620 630 640 650
SRIRSETVAA EDVLQDTGAI SMISSDCQAM GRIGEVVTRT WRTAAKMKQF
660 670 680 690 700
RGPLEGDEPT RDNNRVKRYV AKYTINPAIT HGMSHLIGQV AVGCLADLVF
710 720 730 740 750
WTAESFGARP EMILKGGVIA WAAVGDANAS IPTVQPVLGR PMWGSQPEAA
760 770 780 790 800
ALNSIVWVSQ ASLDKDLVKR FNIKKRAEAV KNCRSIGKKD MKWNDTMPKM
810 820 830
TVDPETYDVR ADGVLCDVPP ADKLPLTRRY FVY
Length:833
Mass (Da):90,475
Last modified:February 6, 2013 - v3
Checksum:i89E4D04C82FFE5ED
GO

Sequence cautioni

The sequence AFR98966 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti249 – 2491G → V in AAC62257 (PubMed:10639402).Curated
Sequence conflicti396 – 3961A → R in AAC62257 (PubMed:10639402).Curated
Sequence conflicti492 – 4921P → A in AAC62257 (PubMed:10639402).Curated
Sequence conflicti700 – 7034FWTA → LLDG in AAC62257 (PubMed:10639402).Curated
Sequence conflicti744 – 75714GSQPE…NSIVW → ALSLRPLHSIQLF in AAC62257 (PubMed:10639402).CuratedAdd
BLAST
Sequence conflicti771 – 7744FNIK → YRLR in AAC62257 (PubMed:10639402).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006062 Genomic DNA. Translation: AAC62257.1.
CP003833 Genomic DNA. Translation: AFR98966.2. Sequence problems.
RefSeqiXP_012053644.1. XM_012198254.1.

Genome annotation databases

EnsemblFungiiAFR98966; AFR98966; CNAG_05540.
GeneIDi23888844.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006062 Genomic DNA. Translation: AAC62257.1.
CP003833 Genomic DNA. Translation: AFR98966.2. Sequence problems.
RefSeqiXP_012053644.1. XM_012198254.1.

3D structure databases

ProteinModelPortaliO13465.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAFR98966; AFR98966; CNAG_05540.
GeneIDi23888844.

Phylogenomic databases

OrthoDBiEOG092C0Z46.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
cd00407. Urease_beta. 1 hit.
cd00390. Urease_gamma. 1 hit.
Gene3Di2.10.150.10. 1 hit.
2.30.40.10. 1 hit.
3.30.280.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR008221. Urease.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR002019. Urease_beta.
IPR002026. Urease_gamma/gamma-beta_su.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
PF00699. Urease_beta. 1 hit.
PF00547. Urease_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF001222. Urease. 1 hit.
PRINTSiPR01752. UREASE.
ProDomiPD002319. Urease_gamma_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51278. SSF51278. 1 hit.
SSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 2 hits.
SSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
TIGR00192. urease_beta. 1 hit.
TIGR00193. urease_gam. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUREA_CRYNH
AccessioniPrimary (citable) accession number: O13465
Secondary accession number(s): J9VZV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: February 6, 2013
Last modified: September 7, 2016
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.