Reviewed, UniProtKB/Swiss-Prot O13444 (ATG15_CLAFU)

Names and origin

Protein names

Recommended name:
    Putative lipase ATG15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	ATG15
Synonyms:	pSI-7

Organism

Cladosporium fulvum (Fulvia fulva)

Taxonomic identifier

5499 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Dothideomycetes › Dothideomycetidae › Capnodiales › Mycosphaerellaceae › mitosporic Mycosphaerellaceae › Passalora

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Induction	During starvation and plant infection. Ref.1
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 661	661	Putative lipase ATG15		PRO_0000317961
Regions
	Topological domain	1 – 3	3	Cytoplasmic By similarity
	Transmembrane	4 – 24	21	Signal-anchor for type II membrane protein
	Topological domain	25 – 661	637	Lumenal By similarity
	Compositional bias	459 – 596	138	Thr-rich
Sites
	Active site	311	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	155	1	N-linked (GlcNAc...) Potential
	Glycosylation	190	1	N-linked (GlcNAc...) Potential
	Glycosylation	212	1	N-linked (GlcNAc...) Potential
	Glycosylation	271	1	N-linked (GlcNAc...) Potential
	Glycosylation	295	1	N-linked (GlcNAc...) Potential
	Glycosylation	457	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O13444-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 94006D2206657847	FASTA	661	71,955
	10 20 30 40 50 60 MIWNGRLVLA CVLLIAGCSG QVDAARTREQ RKAFGSPNIV LPPGPAHEVA SAKPARALGE 70 80 90 100 110 120 KTFTLRHVYH HGTHNYPNLH RYIDIQPESK LQVSYDDGTT YEEAEVAFKA KAQSRIVQRM 130 140 150 160 170 180 AHRSHSDIDA VQVHYFTYGQ PADVEWTEDE IAGPNISDKS TVLTFAKMAA NAYIFSRKDG 190 200 210 220 230 240 EWQPVKGGFN YTDDFGWEQD GLRGHIFADE ANSTVVIGLK GTSPAIFDGA DTTGNDKLND 250 260 270 280 290 300 NLFGSCCCAQ GGPFTWKKVC DCAGSSAYTC NNTCLVKSLR EKGHYYWAVK DLYRNVTERY 310 320 330 340 350 360 PDSEVWLSGH SLGGVVSSLL GLTYGLPTLT FEAFPDAMAA SRLGLPTPPG YKIGSHQARP 370 380 390 400 410 420 MTGIHHFGHT ADPIYMGSCN GGTSFCSIGG YAFEGVCHTG ETCTYDTVRD LGWRVGIGTH 430 440 450 460 470 480 KIVSVIKDVI KVYDQPPSCE QDVECVDCYN WKYFENNGTE TTTSKASTAT STTTRTRTET 490 500 510 520 530 540 CKTPGWWGCL DESTTSAPTT STSTSSSTSS TRTCETPGWF GCKDETTTTS SSSSISSPSA 550 560 570 580 590 600 TPAPTITTTS SLPTSTSTST CKTPGWFGCY DESTTTSATP KPSSTARTVT KTKTTTSDDD 610 620 630 640 650 660 DCTSREWFGL ICVDPSPTTA SGSSPSSTNL PTTRRKMCTK RHWYGTCKQW RFDDFDPKND L

References

[1]

"Starvation-induced genes of the tomato pathogen Cladosporium fulvum are also induced during growth in planta." Coleman M., Henricot B., Arnau J., Oliver R.P. Mol. Plant Microbe Interact. 10:1106-1109(1997) [PubMed: 9390425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: Race 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y14554 mRNA. Translation: CAA74888.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.1.3. 280388.
Family and domain databases
InterPro	IPR008262. Lipase_Ser_AS. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_CLAFU

Accession

Primary (citable) accession number: O13444

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 1, 1998
Last modified:	October 13, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)