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Protein

Isocitrate lyase

Gene

ACU-7

Organism
Coprinopsis cinerea (Inky cap fungus) (Hormographiella aspergillata)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.By similarity

Cofactori

Mg2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi165 – 1651MagnesiumBy similarity
Active sitei203 – 2031Proton acceptorBy similarity
Binding sitei240 – 2401SubstrateBy similarity
Binding sitei454 – 4541SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyase1 Publication (EC:4.1.3.1By similarity)
Short name:
ICLCurated
Short name:
IsocitraseCurated
Short name:
IsocitrataseCurated
Alternative name(s):
Methylisocitrate lyaseBy similarity (EC:4.1.3.30By similarity)
Short name:
MICACurated
Threo-D(S)-isocitrate glyoxylate-lyaseCurated
Gene namesi
Name:ACU-71 Publication
OrganismiCoprinopsis cinerea (Inky cap fungus) (Hormographiella aspergillata)
Taxonomic identifieri5346 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesPsathyrellaceaeCoprinopsis

Subcellular locationi

  • Glyoxysome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 537537Isocitrate lyasePRO_0000068787Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO13439.
SMRiO13439. Positions 1-517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 963Substrate bindingBy similarity
Regioni204 – 2052Substrate bindingBy similarity
Regioni420 – 4245Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi535 – 5373Microbody targeting signalSequence Analysis

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13439-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSERAQFAS EVAEVERWWK SPRFARVNRP YTAADVVSKR GTIKINYPSD
60 70 80 90 100
VQGKKLWKLL SEHAKNGTPS HTYGALDPVQ VTKMAKYLET VYVSGWQSSS
110 120 130 140 150
TASSSNEPGP DLADYPSNTV PNKVEHLFMA QLFHDRKQRE ARSRMSDAEL
160 170 180 190 200
ANTPVIDYLR PIVADADTGH GGLTAVMKLT KMFVEKGAAG IHIEDQAPGT
210 220 230 240 250
KKCGHMAGKV LVPIQEHINR LVAIRLQYDI MGVENLVVAR TDSEAATLIT
260 270 280 290 300
SNIDDRDHPF IQGSTNPSLP PLNNVMVEAE AQGKTGDQLQ AIEDGWIKAA
310 320 330 340 350
NLQLFPQALA QALANEGASR STVEKLVARV SRLSWSQAVA VAQKEFGLKQ
360 370 380 390 400
VPYWNWDAPR TREGYYRYQG GTECAIHRAN AFAPYADLLW METKKPILAQ
410 420 430 440 450
AKEFAAGVHA VHPGQWLAYN LSPSFNWKLL PQRQDMQAYV WELGKLGFVW
460 470 480 490 500
QFITLAGLHS NAYISDLFAQ NFAKTGMKAY VELVQSRERE IGCDVLTHQK
510 520 530
WSGADYADSL IKTVTGGVSS TAAMGAGVTE SQFTSKL
Length:537
Mass (Da):59,358
Last modified:January 1, 1998 - v1
Checksum:iEEAB953191CDFA82
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98860 Genomic DNA. Translation: CAA67367.1.
PIRiJC6182.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98860 Genomic DNA. Translation: CAA67367.1.
PIRiJC6182.

3D structure databases

ProteinModelPortaliO13439.
SMRiO13439. Positions 1-517.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PANTHERiPTHR21631:SF3. PTHR21631:SF3. 1 hit.
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular analysis of the isocitrate lyase gene (acu-7) of the mushroom Coprinus cinereus."
    Chaure P.T., Casselton L.A., Connerton I.F.
    Gene 184:185-187(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: JV6.

Entry informationi

Entry nameiACEA_COPCI
AccessioniPrimary (citable) accession number: O13439
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: May 27, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.