O13395 (CHS6_USTMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 6
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 6
Class-V chitin synthase 6

Gene names

Name:	CHS6
ORF Names:	UM10367, UM03052

Organism

Ustilago maydis (strain 521 / FGSC 9021) (Smut fungus)

Taxonomic identifier

237631 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	1180 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis. Involved in mating tube and dikaryotic hyphae formation. Essential for pathogenicity. Ref.4
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: A constitutive cytoplasmic pool is present that localizes to intracellular microvesicles termed chitosomes. Chitosomes constitute a separate secretory route distinct from the typical secretory pathway and serve as a vehicle for delivering the enzyme to the sites on the cell surface where polysaccharide sythesis takes place. Localizes to septa of yeast-like cells and to the basal septum separating the living tip cell from the vacuolated part in hyphae. Also localizes to the growing bud tip in yeast-like cells and in a tip-ward gradient at the hyphal apex. Ref.3 Ref.4
Sequence similarities	Belongs to the chitin synthase family. Class V subfamily.
Sequence caution	The sequence AAB84285.1 differs from that shown. Reason: Frameshift at position 1067. The sequence EAK84053.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1180	1180	Chitin synthase 6		PRO_0000193724
Regions
	Transmembrane	108 – 128	21	Helical; Potential
	Transmembrane	374 – 394	21	Helical; Potential
	Transmembrane	762 – 782	21	Helical; Potential
	Transmembrane	795 – 815	21	Helical; Potential
	Transmembrane	822 – 842	21	Helical; Potential
Amino acid modifications
	Glycosylation	737	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	196	1	G → R in AAB84285. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O13395 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 72974D483D5F684B	FASTA	1,180	130,623
	10 20 30 40 50 60 MSTKDAHDPF ATPLETITHD PLAETAGLKA DYDKRAYGEA NPALMGTLEK DDMANRPIDA 70 80 90 100 110 120 VEEVPTTSIR KWWVRLTWFT TWWIPSFVLS KCGGMKRPDV QMAWREKFTI CAIIFWLCAI 130 140 150 160 170 180 ILFYIIAFGR LLCPDYDKAW NLSQLSQHAG ENDYYAAVRG TVYDFSKFYK GDHSDITNLQ 190 200 210 220 230 240 TSSDLMLQLA GQDLTGYFPV PLSVGCQSLV SDTSLALMPT ANNTPLISQA IHTSGPLQGD 250 260 270 280 290 300 TSSKLHDINW YPDRFLPFVK KLRKGYYVYS KKDLANQGQW RNWAVIHGKV YDLSNYLNTV 310 320 330 340 350 360 STYQTNPAYS FLDSSIVSLF KSQAGSDITA DFEDAMSTFN QTYRGATQAC LDNVFYVGRT 370 380 390 400 410 420 DFRDTARCEV QNYLLLAFSV LLVTTVLAKF IAALQLGTKR SPEQQDKFVI CQVPCYTEGE 430 440 450 460 470 480 EELRKTIDSL AGLEYDDKRK LLFLICDGMI VGSGNDRSTP RIVLDILGVD PKIDPEPLMF 490 500 510 520 530 540 KSVAEGSKQL NYAKVYSGLY EFEGHVVPYI VVVKVGRPSE RSRPGNRGKR DSQILLMRYL 550 560 570 580 590 600 NRVHFDAPMF PLELEIYHQM KNVIGIDPAF YEYILMVDAD TRVEADGLNR LVANCADDSS 610 620 630 640 650 660 IIAICGETTL DNAEGSWWTM IQVYEYYISH HLSKAFESLF GSVTCLPGCF SLYRIRSSDK 670 680 690 700 710 720 GRPLFISNRI IDDYSENRVD TLHKKNLLHL GEDRYLTTLV LKNFPSFRTK FVPDAKALTS 730 740 750 760 770 780 APDRFGVLLS QRRRWINSTV HNLAELVLMP ELCGFCLFSM RFIVFIDLLG TVILPATAVY 790 800 810 820 830 840 LVYLIVTVAT KSAPIPYISI AMIAAVYGLQ AILFLLKRQW QYIGWLVIYI LAYPVFSFFL 850 860 870 880 890 900 PIYSFWHMDD FSWGNTRIVV GEKGNKKIVA GTDDEPYDDT MIPLKRFSEY QREVWEEEAA 910 920 930 940 950 960 APSMRSGMTG ASGPFGNSQA ILHSGPPSVY RAGGSAYAGS VAGSDYGAGL GDYYQNTNVL 970 980 990 1000 1010 1020 QKPAHSRQTS AAALSQMGGS QAASMMFGTG TPSVYGMAGM GSMYGMPGSS ASMYGLPNPM 1030 1040 1050 1060 1070 1080 MNTTASMYGL PPMLANPLGA NHSPAHSDIG VSMPVSQQNT GGSHIWAQPP EAATVAANSG 1090 1100 1110 1120 1130 1140 RGSGMQARPV STLSALNATN PFGVTAVARA LAVNEASDPT DEEIKSAVQT YLANQPSLMN 1150 1160 1170 1180 VTKRSVREAL VAAFPNAELS YKKSMINKAI DDTLSGGAQA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Umchs5, a gene coding for a class IV chitin synthase in Ustilago maydis." Xoconostle-Cazares B., Specht C.A., Robbins P.W., Liu Y., Leon C., Ruiz-Herrera J. Fungal Genet. Biol. 22:199-208(1997) [PubMed: 9454647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 518.
[2]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.
[3]	"Immunolocalization of chitin synthases in the phytopathogenic dimorphic fungus Ustilago maydis." Ruiz-Herrera J., Xoconostle-Cazares B., Reynaga-Pena C.G., Leon-Ramirez C., Carabez-Trejo A. FEMS Yeast Res. 6:999-1009(2006) [PubMed: 17042749] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis." Weber I., Assmann D., Thines E., Steinberg G. Plant Cell 18:225-242(2006) [PubMed: 16314447] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF030554 Genomic DNA. Translation: AAB84285.1. Frameshift. AACP01000103 Genomic DNA. Translation: EAK84053.1. Sequence problems.
PIR	T42022.
RefSeq	XP_759199.1. XM_754106.1.
3D structure databases
ProteinModelPortal	O13395.
ModBase	Search...
Protein-protein interaction databases
STRING	O13395.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3631121.
KEGG	uma:UM03052.1.
Phylogenomic databases
eggNOG	fuNOG08364.
HOGENOM	HBG738840.
OrthoDB	EOG40S3PT.
Enzyme and pathway databases
BRENDA	2.4.1.16. 6587.
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. IPR001199. Cyt_B5. IPR014876. DEK_C. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 2 hits.
KO	K00698.
Pfam	PF03142. Chitin_synth_2. 1 hit. PF00173. Cyt-b5. 2 hits. PF08766. DEK_C. 1 hit. [Graphical view]
SUPFAM	SSF55856. Cyt_B5. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CHS6_USTMA

Accession

Primary (citable) accession number: O13395
Secondary accession number(s): Q4PA11

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 9, 2007
Last modified:	November 16, 2011
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program