O13394 (CHS5_USTMA) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 73.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Chitin synthase 5 EC=2.4.1.16 Alternative name(s): Chitin-UDP acetyl-glucosaminyl transferase 5 Class-IV chitin synthase 5 | ||||
| Gene names |
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| Organism | Ustilago maydis (strain 521 / FGSC 9021) (Smut fungus) | ||||
| Taxonomic identifier | 237631 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago |
Protein attributes
| Sequence length | 1486 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Plays a major role in cell wall biogenesis. Required for the proper morphology of yeast-like cells. Involved in mating tube and dikaryotic hyphae formation. Essential for pathogenicity. Ref.3 |
| Catalytic activity | UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1). |
| Subcellular location | Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: A constitutive cytoplasmic pool is present that localizes to intracellular microvesicles termed chitosomes. Chitosomes constitute a separate secretory route distinct from the typical secretory pathway and serve as a vehicle for delivering the enzyme to the sites on the cell surface where polysaccharide sythesis takes place By similarity. Localizes to septa of yeast-like cells and to the basal septum separating the living tip cell from the vacuolated part in hyphae. Also localizes to the growing bud tip in yeast-like cells and in a tip-ward gradient at the hyphal apex. Ref.3 |
| Sequence similarities | Belongs to the chitin synthase family. Class IV subfamily. |
| Sequence caution | The sequence AAB84284.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAB84284.1 differs from that shown. Reason: Frameshift at position 1467. The sequence EAK83739.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Cell wall biogenesis/degradation |
| Cellular component | Cell membrane Cytoplasmic vesicle Membrane |
| Domain | Transmembrane Transmembrane helix |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Glycoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW plasma membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | chitin synthase activity Inferred from electronic annotation. Source: EC heme bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1486 | 1486 | Chitin synthase 5 | PRO_0000193723 | |||||
Regions | |||||||||
| Transmembrane | 204 – 224 | 21 | Helical; Potential | ||||||
| Transmembrane | 242 – 262 | 21 | Helical; Potential | ||||||
| Transmembrane | 502 – 522 | 21 | Helical; Potential | ||||||
| Transmembrane | 1054 – 1074 | 21 | Helical; Potential | ||||||
| Transmembrane | 1080 – 1100 | 21 | Helical; Potential | ||||||
| Transmembrane | 1105 – 1125 | 21 | Helical; Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 27 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 49 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 311 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 371 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 409 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 432 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 450 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1379 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1471 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Umchs5, a gene coding for a class IV chitin synthase in Ustilago maydis." Xoconostle-Cazares B., Specht C.A., Robbins P.W., Liu Y., Leon C., Ruiz-Herrera J. Fungal Genet. Biol. 22:199-208(1997) [PubMed: 9454647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 518. |
| [2] | "Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J.  Birren B.W.Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021. |
| [3] | "Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis." Weber I., Assmann D., Thines E., Steinberg G. Plant Cell 18:225-242(2006) [PubMed: 16314447] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF030553 Genomic DNA. Translation: AAB84284.1. Sequence problems. AACP01000086 Genomic DNA. Translation: EAK83739.1. Sequence problems. |
| PIR | T42020. |
| RefSeq | XP_758716.1. XM_753623.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O13394. |
Protein family/group databases | |
| CAZy | GT2. Glycosyltransferase Family 2. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 3630635. |
| KEGG | uma:UM02569.1. |
Phylogenomic databases | |
| eggNOG | fuNOG06113. |
| OrthoDB | EOG4KD9V4. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.16. 6587. |
Family and domain databases | |
| InterPro | IPR004835. Chitin_synth_fng. IPR001199. Cyt_B5. [Graphical view] |
| KO | K00698. |
| Pfam | PF03142. Chitin_synth_2. 1 hit. PF00173. Cyt-b5. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CHS5_USTMA | ||||||||
| Accession | Primary (citable) accession number: O13394 Secondary accession number(s): Q4PBE4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |