Kexin precursor - Candida albicans (strain WO-1) (Yeast)

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O13359 (KEX2_CANAW) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 74. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Kexin
EC=3.4.21.61

Alternative name(s):
KEX2 protease

Gene names

Name:	KEX2
ORF Names:	CAWG_00530

Organism

Candida albicans (strain WO-1) (Yeast)

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	938 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cleavage of -Lys-Arg-\|-Xaa- and -Arg-Arg-\|-Xaa- bonds to process yeast alpha-factor pheromone and killer toxin precursors.
Cofactor	Calcium By similarity.
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein By similarity.
Post-translational modification	O-glycosylated By similarity.
Sequence similarities	Belongs to the peptidase S8 family. Furin subfamily.
Sequence caution	The sequence AAB80929.1 differs from that shown. Reason: Frameshift at position 922.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

20

Potential

Propeptide

21 – ?

Potential

PRO_0000027041

Chain

? – 938

Kexin

PRO_0000027042

Regions

Topological domain

? – 774

Lumenal Potential

Transmembrane

21

Helical; Potential

Topological domain

129

Cytoplasmic Potential

Region

258

Catalytic

Compositional bias

55

Ser/Thr-rich

Compositional bias

4

Poly-Thr

Compositional bias

6

Poly-Arg

Compositional bias

7

Poly-Glu

Sites

Active site

1

Charge relay system By similarity

Active site

1

Charge relay system By similarity

Active site

1

Charge relay system By similarity

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Disulfide bond

By similarity

Disulfide bond

By similarity

Experimental info

Sequence conflict

1

N → D in AAB80929. Ref.1

Sequence conflict

1

F → S in AAB80929. Ref.1

Sequence conflict

1

T → A in AAB80929. Ref.1

Sequence conflict

1

E → EEEE in AAB80929. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

O13359 [UniParc].

Last modified October 19, 2011. Version 2.
Checksum: 7E72BE8AD9FAA9FF
Show »

938

105,144

        10         20         30         40         50         60 
MLPIKLLIFI LGYLLSPTLQ QYQQIPPRDY ENKNYFLVEL NTTNSQKPLI DFISHYRGHY 

        70         80         90        100        110        120 
NFEHQLSSLD NHYVFSIDKS HPHNSFLGNH NSNEYNLMKR QLGHEQDYDE LISHVESIHL 

       130        140        150        160        170        180 
LPMKKLSKRI PVPIEMEDVV FDNRDDTGSD NHEATDEAHQ KLIEIAKKLD IHDPEFTTQW 

       190        200        210        220        230        240 
HLINLKYPGH DVNVTGLWLE NILGQGIVTA LVDDGVDAES DDIKQNFNSE GSWDFNNKGK 

       250        260        270        280        290        300 
SPLPRLFDDY HGTRCAGEIA AVKNDVCGIG VAWKSQVSGI RILSGPITSS DEAEAMVYGL 

       310        320        330        340        350        360 
DTNDIYSCSW GPTDNGKVLS EPDVIVKKAM IKGIQEGRDK KGAIYVFASG NGGRFGDSCN 

       370        380        390        400        410        420 
FDGYTNSIYS ITVGAIDYKG LHPQYSEACS AVMVVTYSSG SGEHIHTTDI KKKCSATHGG 

       430        440        450        460        470        480 
TSAAAPLASG IYSLILSANP NLTWRDVQYI SVLSATPINE EDGNYQTTAL NRKYSHKYGY 

       490        500        510        520        530        540 
GKTDAYKMVH FAKTWVNVKP QAWYYSDIIE VNQTITTTPE QKAPSKRDSP QKIIHSSVNV 

       550        560        570        580        590        600 
SEKDLKIMNV ERVEHITVKV NIDSTYRGRV GMRIISPTGV ISDLATFRVN DASTRGFQNW 

       610        620        630        640        650        660 
TFMSVAHWGE TGIGEWKVEV FVDDSKGDQV EINFKDWQFR IFGESIDGDK AEVYDITKDY 

       670        680        690        700        710        720 
AAIRRELLEK EKQNSKSTTT TSSTTTATTT SGGEGDQKTT TSAENKESTT KVDNSASITT 

       730        740        750        760        770        780 
SQTASLTSSN EQHQPTESNS DSDSDTDDEN KQEGEEDNDN DNDNGNKKAN SDNTGFYLMS 

       790        800        810        820        830        840 
IAVVGFIAVL LVMKFHKTPG SGRRRRRRDG YEFDIIPGED YSDSDDDEDD FDTRRADDDS 

       850        860        870        880        890        900 
FDLGHRNDQR VVSASQQQRQ YDRQQDETRD RLFDDFNAES LPDYENDMFK IGDEEEEEEE 

       910        920        930 
GQQSAKAPSN SEGNSGTSTK KYKDNEADED HKDVVGTQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"KEX2 influences Candida albicans proteinase secretion and hyphal formation." Newport G.R., Agabian N. J. Biol. Chem. 272:28954-28961(1997) [PubMed: 9360967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WO-1.
[2]	"Evolution of pathogenicity and sexual reproduction in eight Candida genomes." Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S., Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I., Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C., Fitzpatrick D.A., de Groot P.W.J. Cuomo C.A. Nature 459:657-662(2009) [PubMed: 19465905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: WO-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF022372 Genomic DNA. Translation: AAB80929.1. Frameshift. CH672346 Genomic DNA. Translation: EEQ42323.1.
3D structure databases
ProteinModelPortal	O13359.
ModBase	Search...
Protein-protein interaction databases
STRING	O13359.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR000209. Peptidase_S8/S53. IPR022398. Peptidase_S8_His-AS. IPR023828. Peptidase_S8_Ser-AS. IPR015500. Peptidase_S8_subtilisin-rel. IPR002884. PrprotnconvertsP. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF01483. P_proprotein. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF52743. Pept_S8_S53. 1 hit.
PROSITE	PS00136. SUBTILASE_ASP. False negative. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KEX2_CANAW

Accession

Primary (citable) accession number: O13359
Secondary accession number(s): C4YDD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	October 19, 2011
Last modified:	November 16, 2011
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents