Reviewed, UniProtKB/Swiss-Prot O13359 (KEX2_CANAL)

Names and origin

Protein names

Recommended name:
    Kexin
    EC=3.4.21.61
Alternative name(s):
    KEX2 protease

Gene names

Name:

KEX2

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	924 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of -Lys-Arg-|-Xaa- and -Arg-Arg-|-Xaa- bonds to process yeast alpha-factor pheromone and killer toxin precursors.
Cofactor	Calcium By similarity.
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein By similarity.
Post-translational modification	O-glycosylated By similarity.
Sequence similarities	Belongs to the peptidase S8 family. Furin subfamily.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal Transmembrane
Ligand	Calcium
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – ?		Potential		PRO_0000027041
	Chain	? – 924		Kexin		PRO_0000027042
Regions
	Topological domain	? – 774		Lumenal Potential
	Transmembrane	775 – 795	21	Potential
	Topological domain	796 – 924	129	Cytoplasmic Potential
	Region	190 – 447	258	Catalytic
	Compositional bias	675 – 729	55	Ser/Thr-rich
	Compositional bias	678 – 681	4	Poly-Thr
	Compositional bias	803 – 808	6	Poly-Arg
	Compositional bias	894 – 903	10	Poly-Glu
Sites
	Active site	213	1	Charge relay system By similarity
	Active site	251	1	Charge relay system By similarity
	Active site	422	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Glycosylation	441	1	N-linked (GlcNAc...) Potential
	Glycosylation	512	1	N-linked (GlcNAc...) Potential
	Glycosylation	539	1	N-linked (GlcNAc...) Potential
	Glycosylation	599	1	N-linked (GlcNAc...) Potential
	Disulfide bond	267 ↔ 414		By similarity
	Disulfide bond	359 ↔ 389		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O13359-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 49E5C2093FCCCCE2	FASTA	924	103,497
	10 20 30 40 50 60 MLPIKLLIFI LGYLLSPTLQ QYQQIPPRDY ENKNYFLVEL NTTNSQKPLI DFISHYRGHY 70 80 90 100 110 120 NFEHQLSSLD NHYVFSIDKS HPHNSFLGNH NSNEYNLMKR QLGHEQDYDE LISHVESIHL 130 140 150 160 170 180 LPMKKLSKRI PVPIEMEDVV FDNRDDTGSD NHEATDEAHQ KLIEIAKKLD IHDPEFTTQW 190 200 210 220 230 240 HLINLKYPGH DVNVTGLWLE DILGQGIVTA LVDDGVDAES DDIKQNFNSE GSWDFNNKGK 250 260 270 280 290 300 SPLPRLFDDY HGTRCAGEIA AVKNDVCGIG VAWKSQVSGI RILSGPITSS DEAEAMVYGL 310 320 330 340 350 360 DTNDIYSCSW GPTDNGKVLS EPDVIVKKAM IKGIQEGRDK KGAIYVFASG NGGRFGDSCN 370 380 390 400 410 420 FDGYTNSIYS ITVGAIDYKG LHPQYSEACS AVMVVTYSSG SGEHIHTTDI KKKCSATHGG 430 440 450 460 470 480 TSAAAPLASG IYSLILSANP NLTWRDVQYI SVLSATPINE EDGNYQTTAL NRKYSHKYGY 490 500 510 520 530 540 GKTDAYKMVH FAKTWVNVKP QAWYYSDIIE VNQTITTTPE QKAPSKRDSP QKIIHSSVNV 550 560 570 580 590 600 SEKDLKIMNV ERVEHITVKV NIDSTYRGRV GMRIISPTGV ISDLATFRVN DASTRGFQNW 610 620 630 640 650 660 TFMSVAHWGE TGIGEWKVEV FVDDSKGDQV EINFKDWQFR IFGESIDGDK AEVYDITKDY 670 680 690 700 710 720 AAIRRELLEK EKQNSKSTTT TSSTTTATTT SGGEGDQKTT TSAENKESTT KVDNSASITT 730 740 750 760 770 780 SQTASLTSSN EQHQPTESNS DSDSDTDDEN KQEGEEDNDN DNDNGNKKAN SDNTGFYLMS 790 800 810 820 830 840 IAVVGFIAVL LVMKFHKTPG SGRRRRRRDG YEFDIIPGED YSDSDDDEDD SDTRRADDDS 850 860 870 880 890 900 FDLGHRNDQR VVSASQQQRQ YDRQQDEARD RLFDDFNAES LPDYENDMFK IGDEEEEEEE 910 920 EEEGQQSAKA PSNSEGNSGT STKK

References

[1]	"KEX2 influences Candida albicans proteinase secretion and hyphal formation." Newport G.R., Agabian N. J. Biol. Chem. 272:28954-28961(1997) [PubMed: 9360967] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WO-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF022372 Genomic DNA. Translation: AAB80929.1.
3D structure databases
HSSP	HSSP built from PDB template 1OT5 based on UniProtKB P13134.
ModBase	Search...
Protein family/group databases
MEROPS	S08.070.
Enzyme and pathway databases
BRENDA	3.4.21.61. 1124.
Family and domain databases
InterPro	IPR000209. Pept_S8_S53. IPR015500. Peptidase_S8_subtilisin-rel. IPR002884. PrprotnconvertsP. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF01483. P_proprotein. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
ProDom	PD000717. PrprotnconvertsP. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00136. SUBTILASE_ASP. False negative. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KEX2_CANAL

Accession

Primary (citable) accession number: O13359

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)