O13356 (CHS1_CRYNV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 1
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 1
Class-IV chitin synthase 1

Gene names

Name:

CHS1

Organism

Cryptococcus neoformans var. grubii (Filobasidiella neoformans var. grubii)

Taxonomic identifier

178876 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Tremellomycetes › Tremellales › Tremellaceae › Filobasidiella › Filobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length	1041 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class IV subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1041	1041	Chitin synthase 1		PRO_0000193689
Regions
	Transmembrane	77 – 97	21	Helical; Potential
	Transmembrane	109 – 129	21	Helical; Potential
	Transmembrane	335 – 355	21	Helical; Potential
	Transmembrane	824 – 844	21	Helical; Potential
	Transmembrane	861 – 881	21	Helical; Potential
	Transmembrane	885 – 905	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	O13356 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 39BADFD0670CE063	FASTA	1,041	116,201
	10 20 30 40 50 60 MSSNQDVPLP FTSSRPLPSR QPTTSRPGGT LKRNKTLTRP ERHVAPAPLV APPTQSFSPT 70 80 90 100 110 120 SPLPQSDGFL NIDWWRLWAY ATTFWAPPVL LRWFGIKEKQ SRQAWREKIA LCWIAVLLGG 130 140 150 160 170 180 VVGFVTMGLQ RALCPADQQN QSMYQRLGDT NMTLSISGWA FNISTSITQS NVDFYSLANQ 190 200 210 220 230 240 MPGQDITDLF TRSVSDYPAC TSSLAYSSGA FCNSTSSSSG TSCVLSSMSQ ETFDSLQIQN 250 260 270 280 290 300 TSLLEGYSWD QVAKLENYMV IDGYVLNMSP YLSFNPTAVE GDEVDSIIRH VLSNQTGSGK 310 320 330 340 350 360 DATRLFFNRQ VPQDAVGCMK ARYVAGRIDK ITPGCFVASF FLYVSLVVIL AVVLARFAMA 370 380 390 400 410 420 CVFNWFLSEK LVKPVTEKEL GRKGINPTIM PEGANVSVNN KLGTAPWAGG GGAAEGKGGG 430 440 450 460 470 480 VGPGKKLVNG KKINPLSPLP SPSHSLPNAP LITLSQIGPE LFTVCLVTCY SEGPSSIRGT 490 500 510 520 530 540 LESIASTNYS DRRKLIWVVC DGMITGQGEK RSTPDVCVGM LDADGRFGNP VPMGYEAVGS 550 560 570 580 590 600 GQKRENRAMV YAGHYVSRNG HRTPTIIVVK CGMPQEATEK KPGNRGKRDS QLILMNFFSR 610 620 630 640 650 660 VTYNDRMTPL DFDLFRKIWT LMGVTPDYFE AVLMVDADTR IYPDSLKHLV NCMHHDNMIM 670 680 690 700 710 720 GVCGETRIAN KRQSWVTAIQ VFEYFISHHH VKAFESVFGG VTCLPGCFSM YRIKARKDGD 730 740 750 760 770 780 NDWVPILVKP EIVNEYSQSE VETLHQKNLL LLGEDRFLST IMLRTFPRRK NIFLPQARCR 790 800 810 820 830 840 TVAPDTFSVL LSQRRRWINS TVHNLMELVR VRDLCGTFCF SMQFVVFMDL VGTVVLPVAI 850 860 870 880 890 900 CLTFALIVNS IITPPKSFEE AIPLMLLAIV LGLPAILILI TTRKVVYVAW MLVYLLALPI 910 920 930 940 950 960 WNFVLPVYSF WHFDDFSWGE TRRVEGEIRS KGHDDSTAVF NGTTIPLRRW EDWEKSRLRK 970 980 990 1000 1010 1020 LRREEKRRKE MERQFGAGFH GDPRLGGDLR GMGMGERPWA RSEYDSDNGS LYSSEEDMWG 1030 1040 GEVGGVSTPF LPSFFPTLLM T

References

[1]	"chs1, a class IV chitin synthase of Cryptococcus neoformans." Specht C.A. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: H99.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF021318 Genomic DNA. Translation: AAB71697.1.
PIR	T31097.
3D structure databases
ProteinModelPortal	O13356.
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. IPR001199. Cyt_B5. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF03142. Chitin_synth_2. 1 hit. PF00173. Cyt-b5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS1_CRYNV

Accession

Primary (citable) accession number: O13356

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	May 31, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program