O13353 (CHS4_MAGO7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 4
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 4
Class-IV chitin synthase 4

Gene names

Name:	CHS4
ORF Names:	MGG_09962

Organism

Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae) [Complete proteome]

Taxonomic identifier

242507 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Magnaporthales › Magnaporthaceae › Magnaporthe

Protein attributes

Sequence length	1229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class IV subfamily.
Sequence caution	The sequence AAB71411.1 differs from that shown. Reason: Frameshift at position 1189.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1229	1229	Chitin synthase 4		PRO_0000193700
Regions
	Transmembrane	241 – 261	21	Helical; Potential
	Transmembrane	496 – 516	21	Helical; Potential
	Transmembrane	1055 – 1075	21	Helical; Potential
	Transmembrane	1089 – 1109	21	Helical; Potential
	Transmembrane	1115 – 1135	21	Helical; Potential
Natural variations
	Natural variant	545 – 546	2	KR → NG in strain: Guyane 11.

Sequences

	Sequence		Length	Mass (Da)
	O13353 [UniParc]. Last modified June 12, 2007. Version 2. Checksum: 388030A667144D55	FASTA	1,229	137,828
	10 20 30 40 50 60 MSLPERPGAK ASYEQRNSYR KSPSRRNRPN DIEASGYYPV TGGQHQRGPS VNSFAETIRS 70 80 90 100 110 120 PNSNIESAPL SPSAEQIEHG SDQPFQRKRS LIRPERNRID RDHPNYHYRK HAAKMNTLPS 130 140 150 160 170 180 STGNDPVLED VSGATESGPP SGSNSASGSG VREENIPRKS RKASGRETVA EKSDNTRRRV 190 200 210 220 230 240 STRNSKIVKE GKRKEKIPEQ LRPPSAWNVY CAVITFWSPD FIMKCCGMPA KAQRRAWREK 250 260 270 280 290 300 IGLISLILII MGVVGFLTFG FNQAVCGGPV LRLHINSVDR SYMIFHGTAY NLDGSHHPVA 310 320 330 340 350 360 EGIPKRLDGT GANVVYDLPE GYGGTDGSFM FQNVNGKCKG LITKAPNSDV PSEGDNLAWY 370 380 390 400 410 420 FPCHARNQDG SSQPNMTYPY YFGYACHTTP LARSTFYTQL DKSADVYFTW ADIRNNSRNL 430 440 450 460 470 480 FVYSGNVLDL DLLFWFNRDQ VNIPRRFEEL RDKNNAANRA IRGRDATRTF MASGDRQIAE 490 500 510 520 530 540 CFEDIIKVGT VDTDTVGCIA AKVVLYVSLA LILSVVGARF TLALIFQWFI SKNYAADKTS 550 560 570 580 590 600 QTSDKRKRNK QIEDWSEDIY RAPPRMPGDV GSSVAGASSS DHTSKRSSFL PTTSRFSTVY 610 620 630 640 650 660 GAERSARNKS MPTTMASQAS GGYMGPSSTA YRETNESRTS FLKSDPYATN TAPIEGPGPS 670 680 690 700 710 720 GFIHDSVVPQ PPSDWMPFGF PLAHTICLVT AYSEGELGLR TTLDSVAMTD YPNSHKVILV 730 740 750 760 770 780 ICDGIIKGKG ESKSTPEYVL DMMKDHTIPV EDVEAFSYVA VASGSKRHNM AKIYAGFYDY 790 800 810 820 830 840 GTQSNIPLDK QQRVPMMVVV KCGTPDEMVK SKPGNRGKRD SQIILMSFLQ KVMFDERMTE 850 860 870 880 890 900 LEYEMFNGLW KVTGISPDFY EIVLMVDADT KVFPDSLTHM ISAMVKDPEI MGLCGETKIA 910 920 930 940 950 960 NKRDSWVSAI QVFEYFISHH LAKSFESVFG GVTCLPGCFC MYRIKAPKGA QNYWVPILAN 970 980 990 1000 1010 1020 PDVVEHYSEN VVDTLHKKNL LLLGEDRYLT TLMLRTFPKR KQVFVPQAVC KTTVPDSFMV 1030 1040 1050 1060 1070 1080 LLSQRRRWIN STIHNLMELV LVRDLCGTFC FSMQFVIFIE LIGTLVLPAA IAFTFYVVII 1090 1100 1110 1120 1130 1140 SIINQPPQII PLVLLGLILG LPAILIIITA HSWSYVLWML IYLLSLPVWN FVLPAYAFWK 1150 1160 1170 1180 1190 1200 FDDFSWGDTR KTAGEKTKKA GIEYEGEFDS SKITMKRWAE FERDRRARQS QYWGSRENVT 1210 1220 GGVRTASGVW ASAPPHHHQQ QYDEYYSDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Specht C.A. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Guyane 11.
[2]	"The genome sequence of the rice blast fungus Magnaporthe grisea." Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K., Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D., Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S., Soanes D.M. , Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M., Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E., Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W. Nature 434:980-986(2005) [PubMed: 15846337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 70-15 / ATCC MYA-4617 / FGSC 8958.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF020528 Genomic DNA. Translation: AAB71411.1. Frameshift. CM001231 Genomic DNA. Translation: EHA57460.1. CM001231 Genomic DNA. Translation: EHA57461.1.
RefSeq	XP_365117.2. XM_365117.2.
UniGene	Mgr.19383.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O13353.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2680870.
KEGG	mgr:MGG_09962.
Phylogenomic databases
OrthoDB	EOG4KD9V4.
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. IPR001199. Cyt_B5. [Graphical view]
KO	K00698.
Pfam	PF03142. Chitin_synth_2. 1 hit. PF00173. Cyt-b5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS4_MAGO7

Accession

Primary (citable) accession number: O13353
Secondary accession number(s): A4R0F5, G4MRN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	June 12, 2007
Last modified:	January 25, 2012
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program