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Protein

Inositol phosphorylceramide synthase

Gene

AUR1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the addition of a phosphorylinositol group onto ceramide to form inositol phosphorylceramide, an essential step in sphingolipid biosynthesis.1 Publication

Enzyme regulationi

Inhibited by aureobasidin A (AbA), khafrefungin and rustmicin.1 Publication

Kineticsi

  1. KM=130 µM for phosphatidylinositol1 Publication
  2. KM=3.3 µM for C(6)-NBD-ceramide1 Publication
  1. Vmax=1864 pmol/min/mg enzyme for phosphatidylinositol1 Publication
  2. Vmax=884 pmol/min/mg enzyme for C(6)-NBD-ceramide1 Publication

GO - Molecular functioni

  • inositol phosphoceramide synthase activity Source: CGD

GO - Biological processi

  • cellular response to drug Source: CGD
  • sphingolipid metabolic process Source: CGD

Keywordsi

Molecular functionTransferase
Biological processLipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

SABIO-RKO13332.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol phosphorylceramide synthase (EC:2.-.-.-)
Short name:
IPC synthase
Alternative name(s):
Aureobasidin A resistance protein homolog
Phosphatidylinositol:ceramide phosphoinositol transferase
Gene namesi
Name:AUR1
Ordered Locus Names:CAALFM_C501240WA
ORF Names:CaO19.1945, CaO19.9500
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Chromosome 5

Organism-specific databases

CGDiCAL0000178882. AUR1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 54CytoplasmicBy similarityAdd BLAST54
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Topological domaini76 – 77LumenalBy similarity2
Transmembranei78 – 98HelicalSequence analysisAdd BLAST21
Topological domaini99 – 100CytoplasmicBy similarity2
Transmembranei101 – 121HelicalSequence analysisAdd BLAST21
Topological domaini122 – 168LumenalBy similarityAdd BLAST47
Transmembranei169 – 189HelicalSequence analysisAdd BLAST21
Topological domaini190 – 191CytoplasmicBy similarity2
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Topological domaini213 – 258LumenalBy similarityAdd BLAST46
Transmembranei259 – 279HelicalSequence analysisAdd BLAST21
Topological domaini280 – 281CytoplasmicBy similarity2
Transmembranei282 – 302HelicalSequence analysisAdd BLAST21
Topological domaini303 – 304LumenalBy similarity2
Transmembranei305 – 325HelicalSequence analysisAdd BLAST21
Topological domaini326 – 471CytoplasmicBy similarityAdd BLAST146

GO - Cellular componenti

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1641343.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000647641 – 471Inositol phosphorylceramide synthaseAdd BLAST471

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi145N-linked (GlcNAc...)Sequence analysis1
Glycosylationi228N-linked (GlcNAc...)Sequence analysis1
Glycosylationi256N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO13332.

Interactioni

Chemistry databases

BindingDBiO13332.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi428 – 434Poly-Glu7

Sequence similaritiesi

Belongs to the AUR1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiO13332.
OrthoDBiEOG092C20GL.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiView protein in InterPro
IPR026841. Aur1/Ipt1.
IPR000326. P_Acid_Pase_2/haloperoxidase.
PfamiView protein in Pfam
PF14378. PAP2_3. 1 hit.
SMARTiView protein in SMART
SM00014. acidPPc. 1 hit.

Sequencei

Sequence statusi: Complete.

O13332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSILRSKI IQKPYQLFHY YFLSEKAPGS TVSDLNFDTN IQTSLRKLKH
60 70 80 90 100
HHWTVGEIFH YGFLVSILFF VFVVFPASFF IKLPIILAFA TCFLIPLTSQ
110 120 130 140 150
FFLPALPVFT WLALYFTCAK IPQEWKPAIT VKVLPAMETI LYGDNLSNVL
160 170 180 190 200
ATITTGVLDI LAWLPYGIIH FSFPFVLAAI IFLFGPPTAL RSFGFAFGYM
210 220 230 240 250
NLLGVLIQMA FPAAPPWYKN LHGLEPANYS MHGSPGGLGR IDKLLGVDMY
260 270 280 290 300
TTGFSNSSII FGAFPSLHSG CCIMEVLFLC WLFPRFKFVW VTYASWLWWS
310 320 330 340 350
TMYLTHHYFV DLIGGAMLSL TVFEFTKYKY LPKNKEGLFC RWSYTEIEKI
360 370 380 390 400
DIQEIDPLSY NYIPINSNDN ESRLYTRVYQ ESQVSPPSRA ETPEAFEMSN
410 420 430 440 450
FSRSRQSSKT QVPLSNLTNN DQVPGINEED EEEEGDEISS STPSVFEDEP
460 470
QGSTYAASSA TSVDDLDSKR N
Length:471
Mass (Da):53,458
Last modified:July 28, 2009 - v2
Checksum:i0CFE68ABF7125A7A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti365I → V in AAB67233 (Ref. 1) Curated1
Sequence conflicti388S → Q in AAB67233 (Ref. 1) Curated1
Sequence conflicti424P → S in AAB67233 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013799 Genomic DNA. Translation: AAB67233.1.
CP017627 Genomic DNA. Translation: AOW29559.1.
RefSeqiXP_715708.1. XM_710615.2.

Genome annotation databases

EnsemblFungiiEAK96634; EAK96634; CaO19.9500.
EAK96693; EAK96693; CaO19.1945.
GeneIDi3642617.
KEGGical:CaO19.1945.
cal:CaO19.9500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF013799 Genomic DNA. Translation: AAB67233.1.
CP017627 Genomic DNA. Translation: AOW29559.1.
RefSeqiXP_715708.1. XM_710615.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiO13332.
ChEMBLiCHEMBL1641343.

Proteomic databases

PRIDEiO13332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAK96634; EAK96634; CaO19.9500.
EAK96693; EAK96693; CaO19.1945.
GeneIDi3642617.
KEGGical:CaO19.1945.
cal:CaO19.9500.

Organism-specific databases

CGDiCAL0000178882. AUR1.

Phylogenomic databases

InParanoidiO13332.
OrthoDBiEOG092C20GL.

Enzyme and pathway databases

SABIO-RKO13332.

Miscellaneous databases

PROiO13332.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiView protein in InterPro
IPR026841. Aur1/Ipt1.
IPR000326. P_Acid_Pase_2/haloperoxidase.
PfamiView protein in Pfam
PF14378. PAP2_3. 1 hit.
SMARTiView protein in SMART
SM00014. acidPPc. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAUR1_CANAL
AccessioniPrimary (citable) accession number: O13332
Secondary accession number(s): A0A1D8PN38, Q5A1Q1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 28, 2009
Last modified: March 15, 2017
This is version 75 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.