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Protein

Homocysteine synthase

Gene

met17

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of O-acetyl-L-homoserine (OAH) into homocysteine in the methionine biosynthesis pathway (PubMed:11754480, PubMed:6526818). Can also use O-succinyl-L-homoserine and L-homoserine as substrates (PubMed:6526818). Has also cysteine synthase (O-acetylserine sulfhydrylase) activity in vitro, but in S.pombe, it seems only to be involved in the alternative pathway of methionine biosynthesis under cysteine deficiency conditions (PubMed:11754480).1 Publication2 Publications

Catalytic activityi

O-acetyl-L-homoserine + methanethiol = L-methionine + acetate.2 Publications

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=12.5 mM for O-acetyl-L-homoserine1 Publication
  2. KM=11.1 mM for O-succinyl-L-homoserine1 Publication
  3. KM=10.4 mM for L-homoserine1 Publication
  4. KM=0.053 mM for H2S1 Publication
  1. Vmax=15.5 µmol/min/mg enzyme for O-acetyl-L-homoserine1 Publication
  2. Vmax=6.2 µmol/min/mg enzyme for O-succinyl-L-homoserinee1 Publication
  3. Vmax=2.5 µmol/min/mg enzyme for L-homoserine1 Publication

pH dependencei

Optimum pH is 8.1 Publication

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in the subpathway that synthesizes L-homocysteine from O-acetyl-L-homoserine.1 Publication This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • cysteine synthase activity Source: PomBase
  • O-acetylhomoserine aminocarboxypropyltransferase activity Source: PomBase
  • pyridoxal phosphate binding Source: GO_Central

GO - Biological processi

  • cysteine biosynthetic process from serine Source: PomBase
  • cysteine biosynthetic process via cystathionine Source: GO_Central
  • L-homocysteine biosynthetic process Source: PomBase
  • transsulfuration Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1593-MONOMER.
RETL1328306-WGS:GSTH-4550-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Homocysteine synthase1 Publication (EC:2.5.1.492 Publications)
Alternative name(s):
O-acetylhomoserine sulfhydrylase2 Publications
Short name:
OAH SHL1 Publication
Short name:
OAH sulfhydrylase1 Publication
Gene namesi
Name:met17By similarity
Synonyms:cys21 Publication
ORF Names:SPBC428.11Imported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC428.11.
PomBaseiSPBC428.11. met17.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi411 – 4111G → D in cys2-1: Impairs homocysteine synthase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Homocysteine synthasePRO_0000114778Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei210 – 2101N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

MaxQBiO13326.

Expressioni

Inductioni

Repressed by cysteine.1 Publication

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi277366. 6 interactions.
MINTiMINT-4666564.

Structurei

3D structure databases

ProteinModelPortaliO13326.
SMRiO13326. Positions 7-426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

HOGENOMiHOG000246417.
InParanoidiO13326.
KOiK17069.
OMAiSHPYHET.
OrthoDBiEOG092C2OYU.
PhylomeDBiO13326.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR006235. OAc-hSer/O-AcSer_sulfhydrylase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 2 hits.
PTHR11808:SF12. PTHR11808:SF12. 2 hits.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01326. OAH_OAS_sulfhy. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVESEHFET LQLHAGQEPD AATSSRAVPI YATTSYVFRD CDHGGRLFGL
60 70 80 90 100
QEPGYIYSRM MNPTADVFEK RIAALEHGAA AIATSSGTSA LFMALTTLAK
110 120 130 140 150
AGDNIVSTSY LYGGTYNLFK VTLPRLGITT KFVNGDDPND LAAQIDENTK
160 170 180 190 200
AVYVESIGNP MYNVPDFERI AEVAHAAGVP LMVDNTFGGG GYLVRPIDHG
210 220 230 240 250
ADIVTHSATK WIGGHGTTIG GVIVDSGKFD WKKNSKRFPE FNEPHPGYHG
260 270 280 290 300
MVFTETFGNL AYAFACRTQT LRDVGGNANP FGVFLLLQGL ETLSLRMERH
310 320 330 340 350
VQNAFALAKY LEKHPKVNWV SYPGLESHVS HKLAKKYLKN GYGAVLSFGA
360 370 380 390 400
KGGPDQSRKV VNALKLASQL ANVGDAKTLV IAPAYTTHLQ LTDEEQISAG
410 420
VTKDLIRVAV GIEHIDDIIA DFAQALEVA
Length:429
Mass (Da):46,409
Last modified:January 1, 1998 - v1
Checksum:i18C717E55087B223
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012876 mRNA. Translation: AAB66879.1.
CU329671 Genomic DNA. Translation: CAA22286.1.
PIRiT40463.
RefSeqiNP_595189.1. NM_001021096.2.

Genome annotation databases

EnsemblFungiiSPBC428.11.1; SPBC428.11.1:pep; SPBC428.11.
GeneIDi2540849.
KEGGispo:SPBC428.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012876 mRNA. Translation: AAB66879.1.
CU329671 Genomic DNA. Translation: CAA22286.1.
PIRiT40463.
RefSeqiNP_595189.1. NM_001021096.2.

3D structure databases

ProteinModelPortaliO13326.
SMRiO13326. Positions 7-426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277366. 6 interactions.
MINTiMINT-4666564.

Proteomic databases

MaxQBiO13326.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC428.11.1; SPBC428.11.1:pep; SPBC428.11.
GeneIDi2540849.
KEGGispo:SPBC428.11.

Organism-specific databases

EuPathDBiFungiDB:SPBC428.11.
PomBaseiSPBC428.11. met17.

Phylogenomic databases

HOGENOMiHOG000246417.
InParanoidiO13326.
KOiK17069.
OMAiSHPYHET.
OrthoDBiEOG092C2OYU.
PhylomeDBiO13326.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1593-MONOMER.
RETL1328306-WGS:GSTH-4550-MONOMER.

Miscellaneous databases

PROiO13326.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR006235. OAc-hSer/O-AcSer_sulfhydrylase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 2 hits.
PTHR11808:SF12. PTHR11808:SF12. 2 hits.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01326. OAH_OAS_sulfhy. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSD_SCHPO
AccessioniPrimary (citable) accession number: O13326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: January 1, 1998
Last modified: September 7, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.