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Protein

Homocysteine synthase

Gene

met17

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of O-acetyl-L-homoserine (OAH) into homocysteine in the methionine biosynthesis pathway (PubMed:11754480, PubMed:6526818). Can also use O-succinyl-L-homoserine and L-homoserine as substrates (PubMed:6526818). Has also cysteine synthase (O-acetylserine sulfhydrylase) activity in vitro, but in S.pombe, it seems only to be involved in the alternative pathway of methionine biosynthesis under cysteine deficiency conditions (PubMed:11754480).1 Publication2 Publications

Catalytic activityi

O-acetyl-L-homoserine + methanethiol = L-methionine + acetate.2 Publications

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=12.5 mM for O-acetyl-L-homoserine1 Publication
  2. KM=11.1 mM for O-succinyl-L-homoserine1 Publication
  3. KM=10.4 mM for L-homoserine1 Publication
  4. KM=0.053 mM for H2S1 Publication
  1. Vmax=15.5 µmol/min/mg enzyme for O-acetyl-L-homoserine1 Publication
  2. Vmax=6.2 µmol/min/mg enzyme for O-succinyl-L-homoserinee1 Publication
  3. Vmax=2.5 µmol/min/mg enzyme for L-homoserine1 Publication

pH dependencei

Optimum pH is 8.1 Publication

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in the subpathway that synthesizes L-homocysteine from O-acetyl-L-homoserine.1 Publication This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • cysteine synthase activity Source: PomBase
  • O-acetylhomoserine aminocarboxypropyltransferase activity Source: PomBase
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • cysteine biosynthetic process from serine Source: PomBase
  • L-homocysteine biosynthetic process Source: PomBase
  • methionine metabolic process Source: PomBase

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandPyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Homocysteine synthase1 Publication (EC:2.5.1.492 Publications)
Alternative name(s):
O-acetylhomoserine sulfhydrylase2 Publications
Short name:
OAH SHL1 Publication
Short name:
OAH sulfhydrylase1 Publication
Gene namesi
Name:met17By similarity
Synonyms:cys21 Publication
ORF Names:SPBC428.11Imported
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC428.11
PomBaseiSPBC428.11 met17

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi411G → D in cys2-1: Impairs homocysteine synthase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001147781 – 429Homocysteine synthaseAdd BLAST429

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei210N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

MaxQBiO13326
PaxDbiO13326
PRIDEiO13326

PTM databases

iPTMnetiO13326

Expressioni

Inductioni

Repressed by cysteine.1 Publication

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi277366, 6 interactors
STRINGi4896.SPBC428.11.1

Structurei

3D structure databases

ProteinModelPortaliO13326
SMRiO13326
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

HOGENOMiHOG000246417
InParanoidiO13326
KOiK17069
OMAiNPRFNIP
OrthoDBiEOG092C2OYU
PhylomeDBiO13326

Family and domain databases

CDDicd00614 CGS_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR000277 Cys/Met-Metab_PyrdxlP-dep_enz
IPR006235 OAc-hSer/O-AcSer_sulfhydrylase
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF01053 Cys_Met_Meta_PP, 1 hit
PIRSFiPIRSF001434 CGS, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR01326 OAH_OAS_sulfhy, 1 hit
PROSITEiView protein in PROSITE
PS00868 CYS_MET_METAB_PP, 1 hit

Sequencei

Sequence statusi: Complete.

O13326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVESEHFET LQLHAGQEPD AATSSRAVPI YATTSYVFRD CDHGGRLFGL
60 70 80 90 100
QEPGYIYSRM MNPTADVFEK RIAALEHGAA AIATSSGTSA LFMALTTLAK
110 120 130 140 150
AGDNIVSTSY LYGGTYNLFK VTLPRLGITT KFVNGDDPND LAAQIDENTK
160 170 180 190 200
AVYVESIGNP MYNVPDFERI AEVAHAAGVP LMVDNTFGGG GYLVRPIDHG
210 220 230 240 250
ADIVTHSATK WIGGHGTTIG GVIVDSGKFD WKKNSKRFPE FNEPHPGYHG
260 270 280 290 300
MVFTETFGNL AYAFACRTQT LRDVGGNANP FGVFLLLQGL ETLSLRMERH
310 320 330 340 350
VQNAFALAKY LEKHPKVNWV SYPGLESHVS HKLAKKYLKN GYGAVLSFGA
360 370 380 390 400
KGGPDQSRKV VNALKLASQL ANVGDAKTLV IAPAYTTHLQ LTDEEQISAG
410 420
VTKDLIRVAV GIEHIDDIIA DFAQALEVA
Length:429
Mass (Da):46,409
Last modified:January 1, 1998 - v1
Checksum:i18C717E55087B223
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012876 mRNA Translation: AAB66879.1
CU329671 Genomic DNA Translation: CAA22286.1
PIRiT40463
RefSeqiNP_595189.1, NM_001021096.2

Genome annotation databases

EnsemblFungiiSPBC428.11.1; SPBC428.11.1:pep; SPBC428.11
GeneIDi2540849
KEGGispo:SPBC428.11

Similar proteinsi

Entry informationi

Entry nameiCYSD_SCHPO
AccessioniPrimary (citable) accession number: O13326
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: January 1, 1998
Last modified: June 20, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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