ID   ADH2_PICST              Reviewed;         348 AA.
AC   O13309; A3LQC2; O00090;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   16-JUN-2009, entry version 54.
DE   RecName: Full=Alcohol dehydrogenase 2;
DE            EC=1.1.1.1;
DE   AltName: Full=Alcohol dehydrogenase II;
DE   AltName: Full=ADH 1;
GN   Name=ADH2; Synonyms=ADH1; ORFNames=PICST_27980;
OS   Pichia stipitis (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Pichia.
OX   NCBI_TaxID=4924;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545;
RX   MEDLINE=98207839; PubMed=9546172;
RA   Cho J.Y., Jeffries T.W.;
RT   "Pichia stipitis genes for alcohol dehydrogenase with fermentative and
RT   respiratory functions.";
RL   Appl. Environ. Microbiol. 64:1350-1358(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 62970 / CBS 5774 / NRRL Y-11542;
RX   MEDLINE=99019018; PubMed=9802210;
RX   DOI=10.1002/(SICI)1097-0061(1998100)14:14<1311::AID-YEA315>3.3.CO;2-K;
RA   Passoth V., Schaefer B., Liebel B., Weierstall T., Klinner U.;
RT   "Molecular cloning of alcohol dehydrogenase genes of the yeast Pichia
RT   stipitis and identification of the fermentative ADH.";
RL   Yeast 14:1311-1325(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-
RT   fermenting yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Converts ethanol to acetaldehyde.
CC   -!- CATALYTIC ACTIVITY: An alcohol + NAD(+) = an aldehyde or ketone +
CC       NADH.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family.
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DR   EMBL; AF008244; AAC49990.1; -; Genomic_DNA.
DR   EMBL; Y13238; CAA73690.1; -; Genomic_DNA.
DR   EMBL; CP000496; ABN65181.1; -; Genomic_DNA.
DR   RefSeq; XP_001383210.1; -.
DR   HSSP; P39462; 1JVB.
DR   SMR; O13309; 2-348.
DR   GeneID; 4837414; -.
DR   KEGG; pic:PICST_27980; -.
DR   OMA; O13309; TEGGPHG.
DR   BRENDA; 1.1.1.1; 81766.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004022; F:alcohol dehydrogenase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013154; ADH_GroES-like.
DR   InterPro; IPR002085; ADH_SF_Zn.
DR   InterPro; IPR013149; ADH_Zn-bd.
DR   InterPro; IPR002328; ADH_Zn_CS.
DR   PANTHER; PTHR11695; ADH_Sf_Zn; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Metal-binding; NAD; Oxidoreductase;
KW   Zinc.
FT   CHAIN         1    348       Alcohol dehydrogenase 2.
FT                                /FTId=PRO_0000160728.
FT   NP_BIND     178    184       NAD (By similarity).
FT   NP_BIND     269    271       NAD (By similarity).
FT   METAL        44     44       Zinc 1; catalytic (By similarity).
FT   METAL        67     67       Zinc 1; catalytic (By similarity).
FT   METAL        98     98       Zinc 2 (By similarity).
FT   METAL       101    101       Zinc 2 (By similarity).
FT   METAL       104    104       Zinc 2 (By similarity).
FT   METAL       112    112       Zinc 2 (By similarity).
FT   METAL       154    154       Zinc 1; catalytic (By similarity).
FT   BINDING     202    202       NAD (By similarity).
FT   BINDING     207    207       NAD (By similarity).
FT   BINDING     341    341       NAD (By similarity).
FT   CONFLICT     41     41       Missing (in Ref. 2; CAA73690).
SQ   SEQUENCE   348 AA;  36565 MW;  F6C813C98F56A148 CRC64;
     MSIPTTQKAV IFETNGGPLL YKDIPVPKPK PNELLINVKY SGVCHTDLHA WKGDWPLDTK
     LPLVGGHEGA GVVVALGENV TGWEIGDYAG IKWINGSCLQ CEYCVTAHES NCPDADLSGY
     THDGSFQQYA TADAIQAARI PKGTDLALIA PILCAGITVY KALKTAQLQA GQWVAVSGAA
     GGLGSLAIQY AKAMGYRVVG IDGGADKGEF AKSLGAEVFV DFLSSKDVVA DVLKATNGGA
     HGVINVSVSE RAMQQSVDYV RPTGTVVLVG LPAGAKVSAS VFSSVVRTIQ IKGSYVGNRA
     DSAEAIDFFT RGLIKCPIKI VGLSELASVY ELMEQGKILG RYVVDTSK
//