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Reviewed, UniProtKB/Swiss-Prot O13309 (ADH2_PICST)

Last modified June 16, 2009. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alcohol dehydrogenase 2
    EC=1.1.1.1
Alternative name(s):
    Alcohol dehydrogenase II
    ADH 1
Gene names
Name: ADH2
Synonyms: ADH1
ORF Names: PICST_27980
OrganismPichia stipitis (Yeast) [Complete proteome]
Taxonomic identifier4924 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

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Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Converts ethanol to acetaldehyde.

Catalytic activity

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family.

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMetal-binding
NAD
Zinc
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionalcohol dehydrogenase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 348348Alcohol dehydrogenase 2
PRO_0000160728

Regions

Nucleotide binding178 – 1847NAD By similarity
Nucleotide binding269 – 2713NAD By similarity

Sites

Metal binding441Zinc 1; catalytic By similarity
Metal binding671Zinc 1; catalytic By similarity
Metal binding981Zinc 2 By similarity
Metal binding1011Zinc 2 By similarity
Metal binding1041Zinc 2 By similarity
Metal binding1121Zinc 2 By similarity
Metal binding1541Zinc 1; catalytic By similarity
Binding site2021NAD By similarity
Binding site2071NAD By similarity
Binding site3411NAD By similarity

Experimental info

Sequence conflict411Missing in CAA73690. Ref.2

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Sequences

Sequence LengthMass (Da)Tools
O13309-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: F6C813C98F56A148

FASTA34836,565
        10         20         30         40         50         60 
MSIPTTQKAV IFETNGGPLL YKDIPVPKPK PNELLINVKY SGVCHTDLHA WKGDWPLDTK 

        70         80         90        100        110        120 
LPLVGGHEGA GVVVALGENV TGWEIGDYAG IKWINGSCLQ CEYCVTAHES NCPDADLSGY 

       130        140        150        160        170        180 
THDGSFQQYA TADAIQAARI PKGTDLALIA PILCAGITVY KALKTAQLQA GQWVAVSGAA 

       190        200        210        220        230        240 
GGLGSLAIQY AKAMGYRVVG IDGGADKGEF AKSLGAEVFV DFLSSKDVVA DVLKATNGGA 

       250        260        270        280        290        300 
HGVINVSVSE RAMQQSVDYV RPTGTVVLVG LPAGAKVSAS VFSSVVRTIQ IKGSYVGNRA 

       310        320        330        340 
DSAEAIDFFT RGLIKCPIKI VGLSELASVY ELMEQGKILG RYVVDTSK

« Hide

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References

« Hide 'large scale' references
[1]"Pichia stipitis genes for alcohol dehydrogenase with fermentative and respiratory functions."
Cho J.Y., Jeffries T.W.
Appl. Environ. Microbiol. 64:1350-1358(1998) [PubMed: 9546172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.
[2]"Molecular cloning of alcohol dehydrogenase genes of the yeast Pichia stipitis and identification of the fermentative ADH."
Passoth V., Schaefer B., Liebel B., Weierstall T., Klinner U.
Yeast 14:1311-1325(1998) [PubMed: 9802210] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 62970 / CBS 5774 / NRRL Y-11542.
[3]"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis."
Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M.
Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

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Cross-references

Sequence databases

AF008244 Genomic DNA. Translation: AAC49990.1.
Y13238 Genomic DNA. Translation: CAA73690.1.
CP000496 Genomic DNA. Translation: ABN65181.1.
RefSeqXP_001383210.1.

3D structure databases

HSSPHSSP built from PDB template 1JVB based on UniProtKB P39462.
SMRO13309. Positions 2-348.
ModBaseSearch...

Genome annotation databases

GeneID4837414.
KEGGpic:PICST_27980.

Phylogenomic databases

OMAO13309. TEGGPHG.

Enzyme and pathway databases

BRENDA1.1.1.1. 81766.

Family and domain databases

InterProIPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn.
IPR013149. ADH_Zn-bd.
IPR002328. ADH_Zn_CS.
[Graphical view]
PANTHERPTHR11695. ADH_Sf_Zn. 1 hit.
PfamPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
PROSITEPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameADH2_PICST
AccessionPrimary (citable) accession number: O13309
Secondary accession number(s): A3LQC2, O00090
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents