Reviewed, UniProtKB/Swiss-Prot O13302 (IDH1_AJECA)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH

Gene names

Name:

IDH1

Organism

Ajellomyces capsulata (Darling's disease fungus) (Histoplasma capsulatum)

Taxonomic identifier

5037 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Onygenales › Ajellomycetaceae › Ajellomyces

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Performs an essential role in the oxidative function of the citric acid cycle By similarity.
Catalytic activity	Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit.
Subunit structure	Octamer of two non-identical subunits IDH1 and IDH2 By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 35	35	Mitochondrion Potential
	Chain	36 – 388	353	Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial		PRO_0000014428
Sites
	Metal binding	255	1	Magnesium or manganese By similarity
	Binding site	137	1	Substrate By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity
	Site	175	1	Critical for catalysis By similarity
	Site	222	1	Critical for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	O13302-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 7E80888D17CEBEF7	FASTA	388	42,222
	10 20 30 40 50 60 MFSLRTAQPA QSLFRAATNT YSTSLPRSAI AARSFATVQS DIFKPTKYGG KYTVTLIPGD 70 80 90 100 110 120 GIGTEVAESV KTIFKADNVP IEWEQVDVSG LDAGNKHSED LFKESIASLK RNKLGLKGIL 130 140 150 160 170 180 HTPVERSGHQ SFNVALRQEL DIYASIVLIK NIPGYKTRHD NVDLCIIREN TEGEYSGLEH 190 200 210 220 230 240 QSVSGVVESL KIITRAKSER IAKFAFSFAL ANNRKKVTCI HKANIMKLAD GLFRSTFHKV 250 260 270 280 290 300 AESYPTLETN DMIVDNASMQ AVARPQQFDV MVMPNLYGGI LSNVGAALVG GPGIVPGCNM 310 320 330 340 350 360 GRDVAVFEPG CRHVGLDIKG KDQANPTALI LSGSMLLRHL GLDEHANRIS KAVYDVIGEG 370 380 VTRTRDMGGQ ASTHEFTRAV LDKMESAL

References

[1]

"Isolation of a Histoplasma capsulatum cDNA that complements a mitochondrial NAD(+)-isocitrate dehydrogenase subunit I-deficient mutant of Saccharomyces cerevisiae." Johnson C.H., McEwen J.E. Yeast 15:799-804(1999) [PubMed: 10398348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC 26032 / G217B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF009036 mRNA. Translation: AAB63461.1.
3D structure databases
HSSP	HSSP built from PDB template 1ISO based on UniProtKB P08200.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.41. 266116.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004434. Isocitrate_DH_NAD_mit. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00175. mito_nad_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH1_AJECA

Accession

Primary (citable) accession number: O13302

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)