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O13294

- IDH2_CANTR

UniProt

O13294 - IDH2_CANTR

Protein

Isocitrate dehydrogenase [NADP] peroxisomal

Gene

IDP2

Organism
Candida tropicalis (Yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    May play a role in N-alkane metabolism, glutamate synthesis, and/or NADPH generation in the peroxisomes.

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei80 – 801SubstrateBy similarity
    Binding sitei85 – 851NADPBy similarity
    Binding sitei112 – 1121SubstrateBy similarity
    Binding sitei135 – 1351SubstrateBy similarity
    Sitei142 – 1421Critical for catalysisBy similarity
    Sitei214 – 2141Critical for catalysisBy similarity
    Metal bindingi254 – 2541Magnesium or manganeseBy similarity
    Binding sitei262 – 2621NADPBy similarity
    Metal bindingi277 – 2771Magnesium or manganeseBy similarity
    Binding sitei330 – 3301NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi78 – 803NADPBy similarity
    Nucleotide bindingi312 – 3176NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-KW
    2. isocitrate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP] peroxisomal (EC:1.1.1.42)
    Short name:
    IDH
    Alternative name(s):
    CtIDP2
    Oxalosuccinate decarboxylase
    PS-NADP-IDH
    Gene namesi
    Name:IDP2
    OrganismiCandida tropicalis (Yeast)
    Taxonomic identifieri5482 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

    Subcellular locationi

    GO - Cellular componenti

    1. peroxisome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 411410Isocitrate dehydrogenase [NADP] peroxisomalPRO_0000083583Add
    BLAST

    Proteomic databases

    PRIDEiO13294.

    Expressioni

    Inductioni

    By N-alkanes.

    Structurei

    3D structure databases

    ProteinModelPortaliO13294.
    SMRiO13294. Positions 6-409.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni97 – 1037Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O13294-1 [UniParc]FASTAAdd to Basket

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    MGEIQKITVK NPIVEMDGDE MTRIIWQFIK DKLILPYLNV DLKYYDLGIE    50
    YRDKTDDKVT TDAAEAILQY GVGVKCATIT PDEARVKEFN LKKMWLSPNG 100
    TLRNVIGGTV FREPIVIDNI PRIVPSWEKP IIIGRHAFGD QYKATDVVIP 150
    AAGDLKLVFK PKDGGEVQEF PVYQFDGPGV ALSMYNTDAS ITDFAESSFQ 200
    LAIERKLNLF SSTKNTILKK YDGKFKDIFE GLYASKYKTK MDELGIWYEH 250
    RLIDDMVAQM LKSKGGYIIA MKNYDGDVQS DIVAQGFGSL GLMTSVLVTP 300
    DGKAFESEAA HGTVTRHYRQ HQQGKETSTN SIASIYAWTR GLIQRGKLDD 350
    TPEVVKFAEE LEKAVIETVS KDNIMTKDLA LTQGKTDRSS YVTTEEFIDG 400
    VANRLNKNLG Y 411
    Length:411
    Mass (Da):46,171
    Last modified:January 23, 2007 - v3
    Checksum:i3E47A093C45F5915
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007994 Genomic DNA. Translation: BAA22846.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007994 Genomic DNA. Translation: BAA22846.1 .

    3D structure databases

    ProteinModelPortali O13294.
    SMRi O13294. Positions 6-409.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O13294.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene analysis of an NADP-linked isocitrate dehydrogenase localized in peroxisomes of the n-alkane-assimilating yeast Candida tropicalis."
      Kawachi H., Shimizu K., Atomi H., Sanuki S., Ueda M., Tanaka A.
      Eur. J. Biochem. 250:205-211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16.
      Strain: ATCC 20336 / pK233 / NCYC 997.

    Entry informationi

    Entry nameiIDH2_CANTR
    AccessioniPrimary (citable) accession number: O13294
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 17, 2003
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 72 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3