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Protein

WD repeat-containing protein srw1

Gene

srw1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in cell differentiation and cell cycling by negatively regulating cig2 and cdc12-associated cdc2. Down-regulates the level of cdc13, particularly in a nitrogen deprived environment. Regulator of cell cycle G1 phase progression. Prevents onset of mitosis during the pre-Start G1 period. Required for degradation of cdc13 mitotic cyclin B during G1 arrest but not during mitotic exit.4 Publications

GO - Molecular functioni

  • anaphase-promoting complex binding Source: InterPro
  • ubiquitin ligase activator activity Source: PomBase

GO - Biological processi

  • mitotic G1 cell cycle arrest in response to nitrogen starvation Source: PomBase
  • positive regulation of anaphase-promoting complex-dependent catabolic process Source: PomBase

Keywordsi

Biological processCell cycle

Enzyme and pathway databases

ReactomeiR-SPO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SPO-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein srw1
Alternative name(s):
Suppressor of rad/wee1
Gene namesi
Name:srw1
Synonyms:ste9
ORF Names:SPAC144.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC144.13c.
PomBaseiSPAC144.13c. srw1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi62S → A: Increased mobility on Western blot. 1 Publication1
Mutagenesisi98T → A: Increased mobility on Western blot. 1 Publication1
Mutagenesisi177T → A: Increased mobility on Western blot. 1 Publication1
Mutagenesisi214S → A: Increased mobility on Western blot. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000512281 – 556WD repeat-containing protein srw1Add BLAST556

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei62Phosphoserine1 Publication1
Modified residuei98Phosphothreonine1 Publication1
Modified residuei177Phosphothreonine1 Publication1
Modified residuei187Phosphoserine1 Publication1
Modified residuei214Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated by cdc2-cdc13-CDK complex. This targets srw1 for proteolysis which in turn promotes cdc13 turnover. Dephosphorylated during G1 arrest.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiO13286.

PTM databases

iPTMnetiO13286.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi279271. 20 interactors.
ELMiO13286.
MINTiMINT-4666474.
STRINGi4896.SPAC144.13c.1.

Structurei

3D structure databases

ProteinModelPortaliO13286.
SMRiO13286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati246 – 285WD 1Add BLAST40
Repeati289 – 328WD 2Add BLAST40
Repeati331 – 368WD 3Add BLAST38
Repeati372 – 411WD 4Add BLAST40
Repeati414 – 456WD 5Add BLAST43
Repeati458 – 499WD 6Add BLAST42
Repeati502 – 541WD 7Add BLAST40

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

HOGENOMiHOG000195514.
InParanoidiO13286.
KOiK03364.
OrthoDBiEOG092C2B79.
PhylomeDBiO13286.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR024977. Apc4_WD40_dom.
IPR033010. Cdc20/Fizzy.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR036322. WD40_repeat_dom_sf.
PANTHERiPTHR19918. PTHR19918. 1 hit.
PfamiView protein in Pfam
PF12894. ANAPC4_WD40. 1 hit.
PF00400. WD40. 3 hits.
SMARTiView protein in SMART
SM00320. WD40. 5 hits.
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiView protein in PROSITE
PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

O13286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDEFDGFTRP TSSNSSANRN SNNSMNRVEN NNSNSDSANT VDSRGDAHTR
60 70 80 90 100
MRQGFEKSFP SSPNKKRPRT NEGDRFIPSR DASTELWTGF TKVEGPLTPV
110 120 130 140 150
KKKQSVADRN FTTLLRSELF GSNDETFNNS PIATPNTTIG VSTPRTDSGI
160 170 180 190 200
DDIELTQRTP PSSSHTSSSI LQNTPVTPSR KIFHYLSPRD RNKSSYGKKA
210 220 230 240 250
QYQDNPNRTI YSLSPVRSIT KDLISASRLE GRELPSIPYR VLDAPGLAGD
260 270 280 290 300
FYLNLLDWGQ CNMLAVALAS RVYLWSGISS EVTVMHNFYP TDTVTSLRWV
310 320 330 340 350
QRGTHLAVGT HNGSVEIWDA ATCKKTRTMS GHTERVGALS WNDHVLSSGG
360 370 380 390 400
RDNHILHRDV RAPEHYFRVL TAHRQEVCGL EWNSNENLLA SGGNDNALMV
410 420 430 440 450
WDKFEEKPLY SFHNHIAAVK AITWSPHQRG ILASGGGTAD RTIKLWNTQR
460 470 480 490 500
GSMLHNIDTG SQVCNLLWSK QTNEFISTHG FMENEVALWN YPSVSRVGTL
510 520 530 540 550
KGHTDRVLYL AMSPNGENIV TGAADETLRF WKLFDSKSKH SASTMSSPFD

PTMKIR
Length:556
Mass (Da):62,060
Last modified:January 1, 1998 - v1
Checksum:i9F9D13EC90F6964E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005589 mRNA. Translation: BAA22152.1.
AB001285 Genomic DNA. Translation: BAF76646.1.
CU329670 Genomic DNA. Translation: CAB59693.1.
PIRiT37680.
RefSeqiNP_594674.1. NM_001020103.2.

Genome annotation databases

EnsemblFungiiSPAC144.13c.1; SPAC144.13c.1:pep; SPAC144.13c.
GeneIDi2542824.
KEGGispo:SPAC144.13c.

Similar proteinsi

Entry informationi

Entry nameiSRW1_SCHPO
AccessioniPrimary (citable) accession number: O13286
Secondary accession number(s): A7VJ97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: January 1, 1998
Last modified: November 22, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families