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O13286

- SRW1_SCHPO

UniProt

O13286 - SRW1_SCHPO

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Protein

WD repeat-containing protein srw1

Gene

srw1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has a role in cell differentiation and cell cycling by negatively regulating cig2 and cdc12-associated cdc2. Down-regulates the level of cdc13, particularly in a nitrogen deprived environment. Regulator of cell cycle G1 phase progression. Prevents onset of mitosis during the pre-Start G1 period. Required for degradation of cdc13 mitotic cyclin B during G1 arrest but not during mitotic exit.4 Publications

GO - Biological processi

  1. activation of mitotic anaphase-promoting complex activity Source: PomBase
  2. cell cycle arrest in response to nitrogen starvation Source: PomBase
  3. cyclin catabolic process Source: PomBase
  4. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: PomBase
  5. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Enzyme and pathway databases

ReactomeiREACT_208775. SCF-beta-TrCP mediated degradation of Emi1.
REACT_246026. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein srw1
Alternative name(s):
Suppressor of rad/wee1
Gene namesi
Name:srw1
Synonyms:ste9
ORF Names:SPAC144.13c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC144.13c.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: PomBase
  2. protein complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi62 – 621S → A: Increased mobility on Western blot. 1 Publication
Mutagenesisi98 – 981T → A: Increased mobility on Western blot. 1 Publication
Mutagenesisi177 – 1771T → A: Increased mobility on Western blot. 1 Publication
Mutagenesisi214 – 2141S → A: Increased mobility on Western blot. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 556556WD repeat-containing protein srw1PRO_0000051228Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621Phosphoserine1 Publication
Modified residuei98 – 981Phosphothreonine1 Publication
Modified residuei177 – 1771Phosphothreonine1 Publication
Modified residuei187 – 1871Phosphoserine1 Publication
Modified residuei214 – 2141Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by cdc2-cdc13-CDK complex. This targets srw1 for proteolysis which in turn promotes cdc13 turnover. Dephosphorylated during G1 arrest.2 Publications

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

BioGridi279271. 18 interactions.
MINTiMINT-4666474.
STRINGi4896.SPAC144.13c-1.

Structurei

3D structure databases

ProteinModelPortaliO13286.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati246 – 28540WD 1Add
BLAST
Repeati289 – 32840WD 2Add
BLAST
Repeati331 – 36838WD 3Add
BLAST
Repeati372 – 41140WD 4Add
BLAST
Repeati414 – 45643WD 5Add
BLAST
Repeati458 – 49942WD 6Add
BLAST
Repeati502 – 54140WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat CDC20/Fizzy family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiCOG2319.
HOGENOMiHOG000195514.
InParanoidiO13286.
KOiK03364.
OMAiNEFISTH.
OrthoDBiEOG7MSMZF.
PhylomeDBiO13286.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13286-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDEFDGFTRP TSSNSSANRN SNNSMNRVEN NNSNSDSANT VDSRGDAHTR
60 70 80 90 100
MRQGFEKSFP SSPNKKRPRT NEGDRFIPSR DASTELWTGF TKVEGPLTPV
110 120 130 140 150
KKKQSVADRN FTTLLRSELF GSNDETFNNS PIATPNTTIG VSTPRTDSGI
160 170 180 190 200
DDIELTQRTP PSSSHTSSSI LQNTPVTPSR KIFHYLSPRD RNKSSYGKKA
210 220 230 240 250
QYQDNPNRTI YSLSPVRSIT KDLISASRLE GRELPSIPYR VLDAPGLAGD
260 270 280 290 300
FYLNLLDWGQ CNMLAVALAS RVYLWSGISS EVTVMHNFYP TDTVTSLRWV
310 320 330 340 350
QRGTHLAVGT HNGSVEIWDA ATCKKTRTMS GHTERVGALS WNDHVLSSGG
360 370 380 390 400
RDNHILHRDV RAPEHYFRVL TAHRQEVCGL EWNSNENLLA SGGNDNALMV
410 420 430 440 450
WDKFEEKPLY SFHNHIAAVK AITWSPHQRG ILASGGGTAD RTIKLWNTQR
460 470 480 490 500
GSMLHNIDTG SQVCNLLWSK QTNEFISTHG FMENEVALWN YPSVSRVGTL
510 520 530 540 550
KGHTDRVLYL AMSPNGENIV TGAADETLRF WKLFDSKSKH SASTMSSPFD

PTMKIR
Length:556
Mass (Da):62,060
Last modified:January 1, 1998 - v1
Checksum:i9F9D13EC90F6964E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005589 mRNA. Translation: BAA22152.1.
AB001285 Genomic DNA. Translation: BAF76646.1.
CU329670 Genomic DNA. Translation: CAB59693.1.
PIRiT37680.
RefSeqiNP_594674.1. NM_001020103.2.

Genome annotation databases

EnsemblFungiiSPAC144.13c.1; SPAC144.13c.1:pep; SPAC144.13c.
GeneIDi2542824.
KEGGispo:SPAC144.13c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005589 mRNA. Translation: BAA22152.1 .
AB001285 Genomic DNA. Translation: BAF76646.1 .
CU329670 Genomic DNA. Translation: CAB59693.1 .
PIRi T37680.
RefSeqi NP_594674.1. NM_001020103.2.

3D structure databases

ProteinModelPortali O13286.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 279271. 18 interactions.
MINTi MINT-4666474.
STRINGi 4896.SPAC144.13c-1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC144.13c.1 ; SPAC144.13c.1:pep ; SPAC144.13c .
GeneIDi 2542824.
KEGGi spo:SPAC144.13c.

Organism-specific databases

PomBasei SPAC144.13c.

Phylogenomic databases

eggNOGi COG2319.
HOGENOMi HOG000195514.
InParanoidi O13286.
KOi K03364.
OMAi NEFISTH.
OrthoDBi EOG7MSMZF.
PhylomeDBi O13286.

Enzyme and pathway databases

Reactomei REACT_208775. SCF-beta-TrCP mediated degradation of Emi1.
REACT_246026. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.

Miscellaneous databases

NextBioi 20803866.
PROi O13286.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
InterProi IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF00400. WD40. 5 hits.
[Graphical view ]
SMARTi SM00320. WD40. 5 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A WD repeat protein controls the cell cycle and differentiation by negatively regulating Cdc2/B-type cyclin complexes."
    Yamaguchi S., Murakami H., Okayama H.
    Mol. Biol. Cell 8:2475-2486(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "Fission yeast ste9, a homolog of hct1/cdh1 and Fizzy-related, is a novel negative regulator of cell cycle progression during G1-phase."
    Kitamura K., Maekawa H., Shimoda C.
    Mol. Biol. Cell 9:1065-1080(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "Apc10 and Ste9/Srw1, two regulators of the APC-cyclosome, as well as the CDK inhibitor Rum1 are required for G1 cell-cycle arrest in fission yeast."
    Kominami K., Seth-Smith H., Toda T.
    EMBO J. 17:5388-5399(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Fission yeast Fizzy-related protein srw1p is a G(1)-specific promoter of mitotic cyclin B degradation."
    Yamaguchi S., Okayama H., Nurse P.
    EMBO J. 19:3968-3977(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT SER-62; THR-98; THR-177 AND SER-214, MUTAGENESIS OF SER-62; THR-98; THR-177 AND SER-214.
  6. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSRW1_SCHPO
AccessioniPrimary (citable) accession number: O13286
Secondary accession number(s): A7VJ97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3