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O13286

- SRW1_SCHPO

UniProt

O13286 - SRW1_SCHPO

Protein

WD repeat-containing protein srw1

Gene

srw1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Has a role in cell differentiation and cell cycling by negatively regulating cig2 and cdc12-associated cdc2. Down-regulates the level of cdc13, particularly in a nitrogen deprived environment. Regulator of cell cycle G1 phase progression. Prevents onset of mitosis during the pre-Start G1 period. Required for degradation of cdc13 mitotic cyclin B during G1 arrest but not during mitotic exit.4 Publications

    GO - Biological processi

    1. activation of mitotic anaphase-promoting complex activity Source: PomBase
    2. cell cycle arrest in response to nitrogen starvation Source: PomBase
    3. cyclin catabolic process Source: PomBase
    4. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: PomBase
    5. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: PomBase

    Keywords - Biological processi

    Cell cycle

    Enzyme and pathway databases

    ReactomeiREACT_208775. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_222028. Regulation of APC/C activators between G1/S and early anaphase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    WD repeat-containing protein srw1
    Alternative name(s):
    Suppressor of rad/wee1
    Gene namesi
    Name:srw1
    Synonyms:ste9
    ORF Names:SPAC144.13c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC144.13c.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: PomBase
    2. protein complex Source: PomBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi62 – 621S → A: Increased mobility on Western blot. 1 Publication
    Mutagenesisi98 – 981T → A: Increased mobility on Western blot. 1 Publication
    Mutagenesisi177 – 1771T → A: Increased mobility on Western blot. 1 Publication
    Mutagenesisi214 – 2141S → A: Increased mobility on Western blot. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 556556WD repeat-containing protein srw1PRO_0000051228Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei62 – 621Phosphoserine1 Publication
    Modified residuei98 – 981Phosphothreonine1 Publication
    Modified residuei177 – 1771Phosphothreonine1 Publication
    Modified residuei187 – 1871Phosphoserine1 Publication
    Modified residuei214 – 2141Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated by cdc2-cdc13-CDK complex. This targets srw1 for proteolysis which in turn promotes cdc13 turnover. Dephosphorylated during G1 arrest.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    BioGridi279271. 18 interactions.
    MINTiMINT-4666474.
    STRINGi4896.SPAC144.13c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliO13286.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati246 – 28540WD 1Add
    BLAST
    Repeati289 – 32840WD 2Add
    BLAST
    Repeati331 – 36838WD 3Add
    BLAST
    Repeati372 – 41140WD 4Add
    BLAST
    Repeati414 – 45643WD 5Add
    BLAST
    Repeati458 – 49942WD 6Add
    BLAST
    Repeati502 – 54140WD 7Add
    BLAST

    Sequence similaritiesi

    Belongs to the WD repeat CDC20/Fizzy family.Curated
    Contains 7 WD repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, WD repeat

    Phylogenomic databases

    eggNOGiCOG2319.
    HOGENOMiHOG000195514.
    KOiK03364.
    OMAiNEFISTH.
    OrthoDBiEOG7MSMZF.
    PhylomeDBiO13286.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    InterProiIPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF00400. WD40. 5 hits.
    [Graphical view]
    SMARTiSM00320. WD40. 5 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 4 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O13286-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDEFDGFTRP TSSNSSANRN SNNSMNRVEN NNSNSDSANT VDSRGDAHTR    50
    MRQGFEKSFP SSPNKKRPRT NEGDRFIPSR DASTELWTGF TKVEGPLTPV 100
    KKKQSVADRN FTTLLRSELF GSNDETFNNS PIATPNTTIG VSTPRTDSGI 150
    DDIELTQRTP PSSSHTSSSI LQNTPVTPSR KIFHYLSPRD RNKSSYGKKA 200
    QYQDNPNRTI YSLSPVRSIT KDLISASRLE GRELPSIPYR VLDAPGLAGD 250
    FYLNLLDWGQ CNMLAVALAS RVYLWSGISS EVTVMHNFYP TDTVTSLRWV 300
    QRGTHLAVGT HNGSVEIWDA ATCKKTRTMS GHTERVGALS WNDHVLSSGG 350
    RDNHILHRDV RAPEHYFRVL TAHRQEVCGL EWNSNENLLA SGGNDNALMV 400
    WDKFEEKPLY SFHNHIAAVK AITWSPHQRG ILASGGGTAD RTIKLWNTQR 450
    GSMLHNIDTG SQVCNLLWSK QTNEFISTHG FMENEVALWN YPSVSRVGTL 500
    KGHTDRVLYL AMSPNGENIV TGAADETLRF WKLFDSKSKH SASTMSSPFD 550
    PTMKIR 556
    Length:556
    Mass (Da):62,060
    Last modified:January 1, 1998 - v1
    Checksum:i9F9D13EC90F6964E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB005589 mRNA. Translation: BAA22152.1.
    AB001285 Genomic DNA. Translation: BAF76646.1.
    CU329670 Genomic DNA. Translation: CAB59693.1.
    PIRiT37680.
    RefSeqiNP_594674.1. NM_001020103.2.

    Genome annotation databases

    EnsemblFungiiSPAC144.13c.1; SPAC144.13c.1:pep; SPAC144.13c.
    GeneIDi2542824.
    KEGGispo:SPAC144.13c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB005589 mRNA. Translation: BAA22152.1 .
    AB001285 Genomic DNA. Translation: BAF76646.1 .
    CU329670 Genomic DNA. Translation: CAB59693.1 .
    PIRi T37680.
    RefSeqi NP_594674.1. NM_001020103.2.

    3D structure databases

    ProteinModelPortali O13286.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279271. 18 interactions.
    MINTi MINT-4666474.
    STRINGi 4896.SPAC144.13c-1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC144.13c.1 ; SPAC144.13c.1:pep ; SPAC144.13c .
    GeneIDi 2542824.
    KEGGi spo:SPAC144.13c.

    Organism-specific databases

    PomBasei SPAC144.13c.

    Phylogenomic databases

    eggNOGi COG2319.
    HOGENOMi HOG000195514.
    KOi K03364.
    OMAi NEFISTH.
    OrthoDBi EOG7MSMZF.
    PhylomeDBi O13286.

    Enzyme and pathway databases

    Reactomei REACT_208775. SCF-beta-TrCP mediated degradation of Emi1.
    REACT_222028. Regulation of APC/C activators between G1/S and early anaphase.

    Miscellaneous databases

    NextBioi 20803866.
    PROi O13286.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    InterProi IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF00400. WD40. 5 hits.
    [Graphical view ]
    SMARTi SM00320. WD40. 5 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS00678. WD_REPEATS_1. 1 hit.
    PS50082. WD_REPEATS_2. 4 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A WD repeat protein controls the cell cycle and differentiation by negatively regulating Cdc2/B-type cyclin complexes."
      Yamaguchi S., Murakami H., Okayama H.
      Mol. Biol. Cell 8:2475-2486(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "Fission yeast ste9, a homolog of hct1/cdh1 and Fizzy-related, is a novel negative regulator of cell cycle progression during G1-phase."
      Kitamura K., Maekawa H., Shimoda C.
      Mol. Biol. Cell 9:1065-1080(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    3. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    4. "Apc10 and Ste9/Srw1, two regulators of the APC-cyclosome, as well as the CDK inhibitor Rum1 are required for G1 cell-cycle arrest in fission yeast."
      Kominami K., Seth-Smith H., Toda T.
      EMBO J. 17:5388-5399(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Fission yeast Fizzy-related protein srw1p is a G(1)-specific promoter of mitotic cyclin B degradation."
      Yamaguchi S., Okayama H., Nurse P.
      EMBO J. 19:3968-3977(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT SER-62; THR-98; THR-177 AND SER-214, MUTAGENESIS OF SER-62; THR-98; THR-177 AND SER-214.
    6. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiSRW1_SCHPO
    AccessioniPrimary (citable) accession number: O13286
    Secondary accession number(s): A7VJ97
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2002
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3