Reviewed, UniProtKB/Swiss-Prot O13283 (XYL1_CANTR)

Names and origin

Protein names

Recommended name:
    NAD(P)H-dependent D-xylose reductase I,II
      Short name=XR
    EC=1.1.1.-

Gene names

Name:

xyrA

Organism

Candida tropicalis (Yeast)

Taxonomic identifier

5482 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces D-xylose into xylitol. Has a preference for NADPH, but can also utilize NADH as cosubstrate By similarity.
Pathway	Carbohydrate metabolism; D-xylose degradation.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Xylose metabolism
Ligand	NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	D-xylose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	NAD(P)H-dependent D-xylose reductase I,II		PRO_0000124662
Regions
	Nucleotide binding	171 – 172	2	NAD or NADP By similarity
	Nucleotide binding	220 – 229	10	NAD or NADP By similarity
	Nucleotide binding	276 – 286	11	NAD or NADP By similarity
Sites
	Active site	54	1	Proton donor By similarity
	Binding site	116	1	Substrate By similarity
	Site	83	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	O13283-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 3002FFBDA21FA3E0	FASTA	324	36,575
	10 20 30 40 50 60 MSTTPTIPTI KLNSGYEMPL VGFGCWKVTN ATAADQIYNA IKTGYRLFDG AEDYGNEKEV 70 80 90 100 110 120 GEGINRAIKE GLVKREELFI TSKLWNNFHD PKNVETALNK TLSDLNLDYV DLFLIHFPIA 130 140 150 160 170 180 FKFVPIEEKY PPGFYCGDGD NFHYEDVPLL DTWKALEKLV EAGKIKSIGI SNFTGALIYD 190 200 210 220 230 240 LIRGATIKPA VLQIEHHPYL QQPKLIEYVQ KAGIAITGYS SFGPQSFLEL ESKRALNTPT 250 260 270 280 290 300 LFEHETIKLI ADKHGKSPAQ VLLRWATQRN IAVIPKSNNP ERLAQNLSVV DFDLTKDDLD 310 320 NIAKLDIGLR FNDPWDWDNI PIFV

References

[1]

"Cloning and sequencing of two D-xylose reductase genes (xyrA and xyrB) from Candida tropicalis." Yokoyama S., Kinoshita Y., Suzuki T., Kawai K., Horitsu H., Takamizawa K. J. Ferment. Bioeng. 80:603-605(1995) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96745 / CBS 4913 / IFO 0618 / NRRL Y-5716.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB002105 Genomic DNA. Translation: BAA19476.1.
3D structure databases
HSSP	HSSP built from PDB template 1K8C based on UniProtKB O74237.
SMR	O13283. Positions 6-324.
ModBase	Search...
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYL1_CANTR

Accession

Primary (citable) accession number: O13283

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)