Reviewed, UniProtKB/Swiss-Prot O13282 (TAF5_SCHPO)

Names and origin

Protein names

Recommended name:
    Transcription initiation factor TFIID subunit 5
Alternative name(s):
    Transcription initiation factor TFIID 72 kDa subunit
      Short name=TAFII-72

Gene names

Name:	taf5
Synonyms:	taf72
ORF Names:	SPCC5E4.03c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	643 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	TAFs are components of the transcription factor IID (TFIID) complex that are essential for mediating regulation of RNA polymerase transcription. Regulates the genes involved in ubiquitin-dependent proteolysis during the progression of M-phase of mitosis. Ref.3
Subunit structure	Component of the TFIID and SAGA complexes. TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs) whose MWs range from 25-150 kDa By similarity. Interacts with gcn5. Interacts (via C-terminal WD repeat-containing region) with taf6.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the WD repeat TAF5 family. Contains 1 LisH domain. Contains 6 WD repeats.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat WD repeat
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation from RNA polymerase II promoter Inferred by curator. Source: GeneDB_SPombe
Cellular component	SAGA complex Ref.4 Inferred from direct assay. Source: GeneDB_SPombe cytosol Inferred from direct assay. Source: GeneDB_SPombe transcription factor TFIID complex Ref.4 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	protein binding Ref.3 Ref.4 Inferred from physical interaction. Source: IntAct transcription initiation factor activity Inferred by curator. Source: GeneDB_SPombe
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
gcn5	Q9UUK2	1	EBI-1152264,EBI-1152314
taf73	O74319	1	EBI-1152264,EBI-1152221

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 643	643	Transcription initiation factor TFIID subunit 5		PRO_0000051259
Regions
	Domain	8 – 40	33	LisH
	Repeat	322 – 361	40	WD 1
	Repeat	377 – 416	40	WD 2
	Repeat	419 – 458	40	WD 3
	Repeat	461 – 500	40	WD 4
	Repeat	503 – 542	40	WD 5
	Repeat	545 – 584	40	WD 6
Amino acid modifications
	Modified residue	256	1	Phosphoserine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	O13282-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 859434527430F3B1	FASTA	643	72,380
	10 20 30 40 50 60 MSATNGPQPQ DLNRIVLDYL AKKGYSRTEA MLRLEASGSG VSVEEKLKSI EETPDAYTHT 70 80 90 100 110 120 YTILRDWVDS SLELYKAELH RILFPIFVHS YLNLLSQDHY EAAKQFYELF KDDHTDLHDF 130 140 150 160 170 180 DVKNLKSLSL PSHVAEDRTA QQYRQNKYQL HFSRITFDLL LHFLFENVSN GGSIIIKLIN 190 200 210 220 230 240 QHIDIHIVPG RPTVLENAKV INEQEGITGQ SFERGDAQLQ PVKLQQMPMD KEMEKIVEMD 250 260 270 280 290 300 LEEEDMMHQN DPNNQSPKLL KEFRKLHEPN AEDAPSRDYI PLPPHKGVDI LSEVEAVKDW 310 320 330 340 350 360 SKRLHLGPRA SLPSVCMYTF HHTNNNMNCA EFSPDSTMIA CGFQESYIRL WSIKADKKSL 370 380 390 400 410 420 PKSTSVEDSD GSVRLLSHSG PVYGTTFSPD NKYLLSCSED ASARLWSVDT KTALVAYKGH 430 440 450 460 470 480 TGPVWDVAFG PFGHYFATAS HDQTAQLWSC DHIYPLRVFA GHLSDVDCVT FHPNSAYVLT 490 500 510 520 530 540 GSSDKTCRLW DVHRGHSVRV FNGHTQPVTA VAIAPDGHTM ASADSEGLIH LWDIGTGRRI 550 560 570 580 590 600 KTMRGHRGNI YSLSFSREST VLVSGGSDCT VRAWDVFKTN YNNPVSSSLT GSVVTPFSAK 610 620 630 640 TSTFNEVNWS TSPDQMVALY TKQTPIFNVS FTRRNLCLAI SVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular genetic elucidation of the tripartite structure of the Schizosaccharomyces pombe 72 kDa TFIID subunit which contains a WD40 structural motif." Yamamoto T., Poon D., Weil P.A., Horikoshi M. Genes Cells 2:245-254(1997) [PubMed: 9224658] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Two WD repeat-containing TATA-binding protein-associated factors in fission yeast that suppress defects in the anaphase-promoting complex." Mitsuzawa H., Seino H., Yamao F., Ishihama A. J. Biol. Chem. 276:17117-17124(2001) [PubMed: 11279037] [Abstract] Cited for: FUNCTION, INTERACTION WITH GCN5.
[4]	"Identification of histone H4-like TAF in Schizosaccharomyces pombe as a protein that interacts with WD repeat-containing TAF." Mitsuzawa H., Ishihama A. Nucleic Acids Res. 30:1952-1958(2002) [PubMed: 11972332] [Abstract] Cited for: INTERACTION WITH TAF6 AND GCN5.
[5]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-256, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB001372 Genomic DNA. Translation: BAA22162.1. CU329672 Genomic DNA. Translation: CAA21958.1.
PIR	T41454.
RefSeq	NP_587902.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	O13282. 5 interactions.
STRING	O13282.
Genome annotation databases
GeneID	2539353.
GenomeReviews	Gene locus taf5 in contig CU329672_GR.
KEGG	spo:SPCC5E4.03c.
NMPDR	fig|4896.1.peg.240.
Organism-specific databases
GeneDB_Spombe	SPCC5E4.03c.
Phylogenomic databases
HOGENOM	HBG559818.
OMA	SVKQASD.
OrthoDB	EOG9MKPZH.
Gene expression databases
ArrayExpress	O13282.
Family and domain databases
InterPro	IPR020472. G-protein_beta_WD-40_rep_reg. IPR006594. LisH_dimerisation. IPR013720. LisH_dimerisation_sub. IPR007582. TFIID-su_WD40-assoc_reg. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR019782. WD40_repeat_2. IPR019775. WD40_repeat_CS. IPR017986. WD40_repeat_dom. IPR019781. WD40_repeat_sg. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF08513. LisH. 1 hit. PF04494. TFIID_90kDa. 1 hit. PF00400. WD40. 6 hits. [Graphical view]
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00667. LisH. 1 hit. SM00320. WD40. 6 hits. [Graphical view]
PROSITE	PS50896. LISH. 1 hit. PS00678. WD_REPEATS_1. 2 hits. PS50082. WD_REPEATS_2. 6 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TAF5_SCHPO

Accession

Primary (citable) accession number: O13282

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2002
Last sequence update:	January 1, 1998
Last modified:	December 15, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)