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Protein

T-box protein VegT-B

Gene

vegt-b

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor required for both mesoderm and endoderm formation in the embryo; signaling determinants and concentration levels may determine which germ layer is formed. Acts together with beta-catenin to activate genes that are responsible for mesoderm induction including wnt-8, eomes t/bra, siamois, mix1 and sox17. Directly binds to promoter DNA. Patterns the mesoderm along the dorsoventral and posterior axis. Activates siamois gene transcription when alone or in combination with beta-catenin, but inhibits siamois transcription in combination with pou5f1.1/oct-25.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi57 – 230174T-boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
T-box protein VegT-B
Alternative name(s):
Xenopus optomotor blind1 Publication
Short name:
XombiImported
Gene namesi
Name:vegt-b
Synonyms:vegt
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-1033836. vegt.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotationBy similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 455455T-box protein VegT-BPRO_0000390488Add
BLAST

Expressioni

Tissue specificityi

Maternally localized to the vegetal hemisphere of oocytes. Zygotic expression parallels blastopore formation and shifts from dorsal expression in the marginal zone of late blastula and early gastrula stages to a ventral/lateral expression at later stages. During neurula and tailbud stages, expressed in the posterior and anterior ends of the embryo. During tailbud stages, expressed in a subset of interneurons in the neural tube.1 Publication

Developmental stagei

Expressed both maternally and zygotically. Expression declines considerably at the end of gastrulation but continues at a low level until the tadpole stage (stage 38).1 Publication

Inductioni

By mesoderm-inducers including t/bra, and both FGF and TGF-beta family members. FGF signaling is not required for initial expression in the dorsal marginal zone, but is required for its continued expression during mid to late gastrula stages.1 Publication

Interactioni

Subunit structurei

Forms a repression complex on the promoters of the nodal/nr1 and siamois genes with the maternal factors tcf7l1/tcf3 and pouf5.1/oct-25. Interacts (via C-terminus) with tcf7l1/tcf3 (via N-terminus). Also interacts with the other POU-domain transcription factors pou5f1.2/oct-91 and pou5f1.3/oct-60 (By similarity).By similarity

Protein-protein interaction databases

BioGridi99325. 1 interaction.
IntActiO13161. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO13161.
SMRiO13161. Positions 48-230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 T-box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG003537.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNCCRERGF SVGRLEPETS FSCASDVKSS PDMDSVSSQD SLYLPNSIGA
60 70 80 90 100
SLEDQNLWTQ FHQEGTEMII TKSGRRMFPQ CKIRLFGLHP YTKYMLLVDF
110 120 130 140 150
VPLDNFRYKW NKNQWEAAGK AEPHPPCRTY VHPDSPASGA HWMKDPICFQ
160 170 180 190 200
KLKLTNNTLD QQGHIILHSM HRYKPRFHVV QSDDMYNSPW GLVQVFSFPE
210 220 230 240 250
TEFTAVTAYQ NEKITKLKIN HNPFAKGFRE QERSHKRDDV LKTLQQSPSK
260 270 280 290 300
SQKRKKWEDS PEADISDFPK ATRIKEESIM DPAGVYQNWV SDHEANQGLT
310 320 330 340 350
PHSPESEGVN QEQQVPTSSS NFYIKSQYRR SSQHLSSPYD LGEPSSRRLT
360 370 380 390 400
PDVATVPDSD PDSLAVLHVI PTQNSAQERT CSMNFSMETP MKQPLRGAIY
410 420 430 440 450
SPYGTEQWMV PAQGPYQPVS YTAYPTDLSA QGAVAHPHSG MSDWSQYSLF

PYSCW
Length:455
Mass (Da):51,963
Last modified:July 1, 1997 - v1
Checksum:i2B9C8FBCCD78E37D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S83518 mRNA. Translation: AAB50917.1.
BC070708 mRNA. Translation: AAH70708.1.
RefSeqiNP_001081671.1. NM_001088202.1.
UniGeneiXl.3520.

Genome annotation databases

GeneIDi397990.
KEGGixla:397990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S83518 mRNA. Translation: AAB50917.1.
BC070708 mRNA. Translation: AAH70708.1.
RefSeqiNP_001081671.1. NM_001088202.1.
UniGeneiXl.3520.

3D structure databases

ProteinModelPortaliO13161.
SMRiO13161. Positions 48-230.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi99325. 1 interaction.
IntActiO13161. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397990.
KEGGixla:397990.

Organism-specific databases

CTDi397990.
XenbaseiXB-GENE-1033836. vegt.

Phylogenomic databases

HOVERGENiHBG003537.

Family and domain databases

Gene3Di2.60.40.820. 1 hit.
InterProiIPR008967. p53-like_TF_DNA-bd.
IPR001699. TF_T-box.
IPR018186. TF_T-box_CS.
[Graphical view]
PANTHERiPTHR11267. PTHR11267. 1 hit.
PfamiPF00907. T-box. 1 hit.
[Graphical view]
PRINTSiPR00937. TBOX.
SMARTiSM00425. TBOX. 1 hit.
[Graphical view]
SUPFAMiSSF49417. SSF49417. 1 hit.
PROSITEiPS01283. TBOX_1. 1 hit.
PS01264. TBOX_2. 1 hit.
PS50252. TBOX_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning of a Xenopus T-related gene (Xombi) involved in mesodermal patterning and blastopore lip formation."
    Lustig K.D., Kroll K.L., Sun E.E., Kirschner M.W.
    Development 122:4001-4012(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: OocyteImported.
  3. "Evidence for dual mechanisms of mesoderm establishment in Xenopus embryos."
    Kavka A.I., Green J.B.
    Dev. Dyn. 219:77-83(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiVEGTB_XENLA
AccessioniPrimary (citable) accession number: O13161
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: July 1, 1997
Last modified: May 11, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.