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O13154

- PACN2_CHICK

UniProt

O13154 - PACN2_CHICK

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Protein
Protein kinase C and casein kinase substrate in neurons protein 2
Gene
PACSIN2
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane and thereby may play a role in caveola-mediated endocytosis By similarity.

GO - Molecular functioni

  1. cytoskeletal protein binding Source: AgBase
  2. phosphatidic acid binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. actin cytoskeleton organization Source: InterPro
  2. caveola assembly Source: InterPro
  3. endocytosis Source: UniProtKB-KW
  4. membrane tubulation Source: InterPro
  5. negative regulation of endocytosis Source: AgBase
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase substrate in neurons protein 2
Alternative name(s):
Focal adhesion protein of 52 kDa
Short name:
FAP52
Gene namesi
Name:PACSIN2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Cell junctionfocal adhesion. Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Early endosome By similarity. Recycling endosome membrane By similarity. Cell projectionruffle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell projection By similarity. Membranecaveola By similarity
Note: Detected at the neck of flask-shaped caveolae By similarity.1 Publication

GO - Cellular componenti

  1. caveola Source: UniProtKB-SubCell
  2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  3. cytoskeleton Source: UniProtKB-SubCell
  4. cytosol Source: AgBase
  5. early endosome Source: UniProtKB-SubCell
  6. focal adhesion Source: UniProtKB-SubCell
  7. recycling endosome membrane Source: UniProtKB-SubCell
  8. ruffle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448Protein kinase C and casein kinase substrate in neurons protein 2
PRO_0000161798Add
BLAST

Post-translational modificationi

Phosphorylated on serine residues.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO13154.
PRIDEiO13154.

Expressioni

Tissue specificityi

Detected in intestine, cardiac muscle, lung and brain (at protein level). Expressed in all tissues tested, including, gizzard, liver, cardiac muscle, skeletal muscle and skin.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000022861.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni21 – 244
Helixi25 – 7248
Helixi77 – 10630
Helixi108 – 11912
Beta strandi126 – 1283
Helixi129 – 17446
Turni175 – 1784
Helixi184 – 25572
Turni257 – 2593
Helixi261 – 27515
Helixi279 – 29012

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BNEX-ray2.57A/B1-448[»]
ProteinModelPortaliO13154.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 7565FCH
Add
BLAST
Domaini388 – 44861SH3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 308308F-BAR domain By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili25 – 274250 By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi367 – 3693NPF1
Motifi379 – 3813NPF2

Domaini

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain By similarity.

Sequence similaritiesi

Belongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain.

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG283356.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
PhylomeDBiO13154.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR028521. PACSIN2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10959:SF2. PTHR10959:SF2. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O13154-1 [UniParc]FASTAAdd to Basket

« Hide

MSGSYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCND LMNCIHERAR    50
IEKVYAQQLT EWAKRWKQLV EKGPQYGTVE RAWCAFMSEA EKVSELHLEV 100
KGSLMNEDFE KIKNWQKEAF HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK 150
EVEAAKKAYH AACKEEKLAI SRETNSKADP ALNPEQLKKL QDKVERSKQD 200
VLKTKAKYEK SLKELDNATP QYMENMEQVF EQCQQFEEKR LRFFREVLLE 250
VQKHLDLSNV ASYKNIYREL EQNIKTADAV EDLRWFRANQ GPGMSMNWPQ 300
FEDDEWSADL NRTLSRREKK KASDGVTLTG INQTGDQVSQ PNKHSSVSSY 350
EKNQSYPTDW SDEESNNPFS STDAKGDTNP FDEDTSPVME VRVRALYDYE 400
GQEQDELSFK AGDELTKMEN EDEQGWCKGR LDNGQVGLYP ANYVEPIQ 448
Length:448
Mass (Da):51,971
Last modified:July 1, 1997 - v1
Checksum:i344218153F8698EF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50798 mRNA. Translation: CAA90678.1.
RefSeqiNP_001152983.1. NM_001159511.1.
NP_990420.1. NM_205089.1.
UniGeneiGga.3831.

Genome annotation databases

GeneIDi395975.
KEGGigga:395975.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z50798 mRNA. Translation: CAA90678.1 .
RefSeqi NP_001152983.1. NM_001159511.1.
NP_990420.1. NM_205089.1.
UniGenei Gga.3831.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4BNE X-ray 2.57 A/B 1-448 [» ]
ProteinModelPortali O13154.
ModBasei Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000022861.

Proteomic databases

PaxDbi O13154.
PRIDEi O13154.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 395975.
KEGGi gga:395975.

Organism-specific databases

CTDi 11252.

Phylogenomic databases

eggNOGi NOG283356.
HOGENOMi HOG000007245.
HOVERGENi HBG053486.
PhylomeDBi O13154.

Miscellaneous databases

NextBioi 20816040.
PROi O13154.

Family and domain databases

InterProi IPR001060. FCH_dom.
IPR028521. PACSIN2.
IPR001452. SH3_domain.
[Graphical view ]
PANTHERi PTHR10959:SF2. PTHR10959:SF2. 1 hit.
Pfami PF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
SMARTi SM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "FAP52, a novel, SH3 domain-containing focal adhesion protein."
    Merilaeinen J., Lehto V.-P., Wasenius V.-M.
    J. Biol. Chem. 272:23278-23284(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION, TISSUE SPECIFICITY.
    Tissue: Brain.

Entry informationi

Entry nameiPACN2_CHICK
AccessioniPrimary (citable) accession number: O13154
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: July 1, 1997
Last modified: September 3, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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