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O13154 (PACN2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein kinase C and casein kinase substrate in neurons protein 2
Alternative name(s):
Focal adhesion protein of 52 kDa
Short name=FAP52
Gene names
Name:PACSIN2
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane and thereby may play a role in caveola-mediated endocytosis By similarity.

Subcellular location

Cell junctionfocal adhesion. Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Early endosome By similarity. Recycling endosome membrane By similarity. Cell projectionruffle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell projection By similarity. Membranecaveola By similarity. Note: Detected at the neck of flask-shaped caveolae By similarity. Ref.1

Tissue specificity

Detected in intestine, cardiac muscle, lung and brain (at protein level). Expressed in all tissues tested, including, gizzard, liver, cardiac muscle, skeletal muscle and skin. Ref.1

Domain

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain By similarity.

Post-translational modification

Phosphorylated on serine residues. Ref.1

Sequence similarities

Belongs to the PACSIN family.

Contains 1 FCH domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Biological processEndocytosis
   Cellular componentCell junction
Cell membrane
Cell projection
Cytoplasm
Cytoplasmic vesicle
Cytoskeleton
Endosome
Membrane
   DomainCoiled coil
SH3 domain
   LigandLipid-binding
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin cytoskeleton organization

Inferred from electronic annotation. Source: InterPro

caveola assembly

Inferred from electronic annotation. Source: InterPro

endocytosis

Inferred from electronic annotation. Source: UniProtKB-KW

membrane tubulation

Inferred from electronic annotation. Source: InterPro

negative regulation of endocytosis

Inferred from sequence or structural similarity. Source: AgBase

   Cellular_componentcaveola

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytosol

Inferred from sequence or structural similarity. Source: AgBase

early endosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

focal adhesion

Inferred from electronic annotation. Source: UniProtKB-SubCell

recycling endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

ruffle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncytoskeletal protein binding

Inferred from sequence or structural similarity. Source: AgBase

phosphatidic acid binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 448448Protein kinase C and casein kinase substrate in neurons protein 2
PRO_0000161798

Regions

Domain11 – 7565FCH
Domain388 – 44861SH3
Region1 – 308308F-BAR domain By similarity
Coiled coil25 – 274250 By similarity
Motif367 – 3693NPF1
Motif379 – 3813NPF2

Sequences

Sequence LengthMass (Da)Tools
O13154 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 344218153F8698EF

FASTA44851,971
        10         20         30         40         50         60 
MSGSYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCND LMNCIHERAR IEKVYAQQLT 

        70         80         90        100        110        120 
EWAKRWKQLV EKGPQYGTVE RAWCAFMSEA EKVSELHLEV KGSLMNEDFE KIKNWQKEAF 

       130        140        150        160        170        180 
HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK EVEAAKKAYH AACKEEKLAI SRETNSKADP 

       190        200        210        220        230        240 
ALNPEQLKKL QDKVERSKQD VLKTKAKYEK SLKELDNATP QYMENMEQVF EQCQQFEEKR 

       250        260        270        280        290        300 
LRFFREVLLE VQKHLDLSNV ASYKNIYREL EQNIKTADAV EDLRWFRANQ GPGMSMNWPQ 

       310        320        330        340        350        360 
FEDDEWSADL NRTLSRREKK KASDGVTLTG INQTGDQVSQ PNKHSSVSSY EKNQSYPTDW 

       370        380        390        400        410        420 
SDEESNNPFS STDAKGDTNP FDEDTSPVME VRVRALYDYE GQEQDELSFK AGDELTKMEN 

       430        440 
EDEQGWCKGR LDNGQVGLYP ANYVEPIQ 

« Hide

References

[1]"FAP52, a novel, SH3 domain-containing focal adhesion protein."
Merilaeinen J., Lehto V.-P., Wasenius V.-M.
J. Biol. Chem. 272:23278-23284(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION, TISSUE SPECIFICITY.
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z50798 mRNA. Translation: CAA90678.1.
RefSeqNP_001152983.1. NM_001159511.1.
NP_990420.1. NM_205089.1.
UniGeneGga.3831.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4BNEX-ray2.57A/B1-448[»]
ProteinModelPortalO13154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000022861.

Proteomic databases

PaxDbO13154.
PRIDEO13154.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID395975.
KEGGgga:395975.

Organism-specific databases

CTD11252.

Phylogenomic databases

eggNOGNOG283356.
HOGENOMHOG000007245.
HOVERGENHBG053486.
PhylomeDBO13154.

Family and domain databases

InterProIPR001060. FCH_dom.
IPR028521. PACSIN2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR10959:SF2. PTHR10959:SF2. 1 hit.
PfamPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20816040.
PROO13154.

Entry information

Entry namePACN2_CHICK
AccessionPrimary (citable) accession number: O13154
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: July 1, 1997
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references