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O13154

- PACN2_CHICK

UniProt

O13154 - PACN2_CHICK

Protein

Protein kinase C and casein kinase substrate in neurons protein 2

Gene

PACSIN2

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
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    Functioni

    Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane and thereby may play a role in caveola-mediated endocytosis By similarity.By similarity

    GO - Molecular functioni

    1. cytoskeletal protein binding Source: AgBase
    2. phosphatidic acid binding Source: UniProtKB

    GO - Biological processi

    1. actin cytoskeleton organization Source: InterPro
    2. caveola assembly Source: InterPro
    3. endocytosis Source: UniProtKB-KW
    4. membrane tubulation Source: InterPro
    5. negative regulation of endocytosis Source: AgBase

    Keywords - Biological processi

    Endocytosis

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein kinase C and casein kinase substrate in neurons protein 2
    Alternative name(s):
    Focal adhesion protein of 52 kDa
    Short name:
    FAP52
    Gene namesi
    Name:PACSIN2
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Cell junctionfocal adhesion 1 Publication. Cytoplasm By similarity. Cytoplasmcytoskeleton By similarity. Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Early endosome By similarity. Recycling endosome membrane By similarity. Cell projectionruffle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cell projection By similarity. Membranecaveola By similarity
    Note: Detected at the neck of flask-shaped caveolae.By similarity

    GO - Cellular componenti

    1. caveola Source: UniProtKB-SubCell
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. cytoskeleton Source: UniProtKB-SubCell
    4. cytosol Source: AgBase
    5. early endosome Source: UniProtKB-SubCell
    6. focal adhesion Source: UniProtKB-SubCell
    7. recycling endosome membrane Source: UniProtKB-SubCell
    8. ruffle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 448448Protein kinase C and casein kinase substrate in neurons protein 2PRO_0000161798Add
    BLAST

    Post-translational modificationi

    Phosphorylated on serine residues.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO13154.
    PRIDEiO13154.

    Expressioni

    Tissue specificityi

    Detected in intestine, cardiac muscle, lung and brain (at protein level). Expressed in all tissues tested, including, gizzard, liver, cardiac muscle, skeletal muscle and skin.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000022861.

    Structurei

    Secondary structure

    1
    448
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni21 – 244
    Helixi25 – 7248
    Helixi77 – 10630
    Helixi108 – 11912
    Beta strandi126 – 1283
    Helixi129 – 17446
    Turni175 – 1784
    Helixi184 – 25572
    Turni257 – 2593
    Helixi261 – 27515
    Helixi279 – 29012

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4BNEX-ray2.57A/B1-448[»]
    ProteinModelPortaliO13154.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 7565FCHPROSITE-ProRule annotationAdd
    BLAST
    Domaini388 – 44861SH3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 308308F-BAR domainBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili25 – 274250By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi367 – 3693NPF1
    Motifi379 – 3813NPF2

    Domaini

    The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain By similarity.By similarity

    Sequence similaritiesi

    Belongs to the PACSIN family.Curated
    Contains 1 FCH domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    eggNOGiNOG283356.
    HOGENOMiHOG000007245.
    HOVERGENiHBG053486.
    PhylomeDBiO13154.

    Family and domain databases

    InterProiIPR001060. FCH_dom.
    IPR028521. PACSIN2.
    IPR001452. SH3_domain.
    [Graphical view]
    PANTHERiPTHR10959:SF2. PTHR10959:SF2. 1 hit.
    PfamiPF00611. FCH. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O13154-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGSYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCND LMNCIHERAR    50
    IEKVYAQQLT EWAKRWKQLV EKGPQYGTVE RAWCAFMSEA EKVSELHLEV 100
    KGSLMNEDFE KIKNWQKEAF HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK 150
    EVEAAKKAYH AACKEEKLAI SRETNSKADP ALNPEQLKKL QDKVERSKQD 200
    VLKTKAKYEK SLKELDNATP QYMENMEQVF EQCQQFEEKR LRFFREVLLE 250
    VQKHLDLSNV ASYKNIYREL EQNIKTADAV EDLRWFRANQ GPGMSMNWPQ 300
    FEDDEWSADL NRTLSRREKK KASDGVTLTG INQTGDQVSQ PNKHSSVSSY 350
    EKNQSYPTDW SDEESNNPFS STDAKGDTNP FDEDTSPVME VRVRALYDYE 400
    GQEQDELSFK AGDELTKMEN EDEQGWCKGR LDNGQVGLYP ANYVEPIQ 448
    Length:448
    Mass (Da):51,971
    Last modified:July 1, 1997 - v1
    Checksum:i344218153F8698EF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50798 mRNA. Translation: CAA90678.1.
    RefSeqiNP_001152983.1. NM_001159511.1.
    NP_990420.1. NM_205089.1.
    UniGeneiGga.3831.

    Genome annotation databases

    GeneIDi395975.
    KEGGigga:395975.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z50798 mRNA. Translation: CAA90678.1 .
    RefSeqi NP_001152983.1. NM_001159511.1.
    NP_990420.1. NM_205089.1.
    UniGenei Gga.3831.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4BNE X-ray 2.57 A/B 1-448 [» ]
    ProteinModelPortali O13154.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000022861.

    Proteomic databases

    PaxDbi O13154.
    PRIDEi O13154.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 395975.
    KEGGi gga:395975.

    Organism-specific databases

    CTDi 11252.

    Phylogenomic databases

    eggNOGi NOG283356.
    HOGENOMi HOG000007245.
    HOVERGENi HBG053486.
    PhylomeDBi O13154.

    Miscellaneous databases

    NextBioi 20816040.
    PROi O13154.

    Family and domain databases

    InterProi IPR001060. FCH_dom.
    IPR028521. PACSIN2.
    IPR001452. SH3_domain.
    [Graphical view ]
    PANTHERi PTHR10959:SF2. PTHR10959:SF2. 1 hit.
    Pfami PF00611. FCH. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "FAP52, a novel, SH3 domain-containing focal adhesion protein."
      Merilaeinen J., Lehto V.-P., Wasenius V.-M.
      J. Biol. Chem. 272:23278-23284(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION, TISSUE SPECIFICITY.
      Tissue: Brain.

    Entry informationi

    Entry nameiPACN2_CHICK
    AccessioniPrimary (citable) accession number: O13154
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 2002
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3