Reviewed, UniProtKB/Swiss-Prot O13148 (EPA4A_DANRE)

Names and origin

Protein names

Recommended name:
    Ephrin type-A receptor 4a
    EC=2.7.10.1
Alternative name(s):
    Tyrosine-protein kinase receptor ZEK2
    EPH-like kinase 2

Gene names

Name:	epha4a
Synonyms:	ek2, zek2

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	292 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for members of the ephrin-B family. May play a role in early pattern formation within the developing nervous system.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Widely expressed in the developing nervous system.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW transmembrane receptor protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›292	›292	Ephrin type-A receptor 4a		PRO_0000160272
Regions
	Domain	‹1 – 265	›265	Protein kinase
	Nucleotide binding	1 – 9	9	ATP By similarity
Sites
	Active site	120	1	Proton acceptor By similarity
	Binding site	27	1	ATP By similarity
Amino acid modifications
	Modified residue	153	1	Phosphotyrosine; by autocatalysis Potential
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	292	1

Sequences

	Sequence		Length	Mass (Da)
	O13148-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 923879877EF13879	FASTA	292	33,045
	10 20 30 40 50 60 IGIGEFGEVC SGRLKMPGKR EICVAIKTLK AGYTDKQRRD FLSEASIMGQ FDHPNIIRLE 70 80 90 100 110 120 GVVTKCKPVM IITEYMENGS LDAFLRKNDG RFTVIQLVGI LRGIASGMKY LSDMSYVHRD 130 140 150 160 170 180 LAARNILVNS NLVCKVSDFG MSRVLEEDPD AAYTTREITG TYQSQGGKIP IRWTAPEAIT 190 200 210 220 230 240 YRKFTSASDV WSYGIVMWEV MSYGERPYWD MSNQDVIKAI EEGYRLPPPM DCPVSLHQLM 250 260 270 280 290 LDCWQKERAE RPKFSQIVNM LDKLIRNPNS LKRTGGEIAR PNTTLLEPSS PE

References

[1]	"Novel Eph-family receptor tyrosine kinase is widely expressed in the developing zebrafish nervous system." Bovenkamp D.E., Greer P. Dev. Dyn. 209:166-181(1997) [PubMed: 9186052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U89380 mRNA. Translation: AAC60222.1.
IPI	IPI00632580.
UniGene	Dr.113940 Dr.81906
3D structure databases
HSSP	HSSP built from PDB template 1JPA based on UniProtKB P54763.
SMR	O13148. Positions 1-271.
ModBase	Search...
Organism-specific databases
ZFIN	ZDB-GENE-001207-7. epha4a.
Phylogenomic databases
HOGENOM	O13148.
HOVERGEN	O13148.
Enzyme and pathway databases
BRENDA	2.7.10.1. 96826.
Gene expression databases
ArrayExpress	O13148.
Bgee	O13148.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EPA4A_DANRE

Accession

Primary (citable) accession number: O13148

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project