Reviewed, UniProtKB/Swiss-Prot O13146 (EPHA3_DANRE)

Names and origin

Protein names

Recommended name:
    Ephrin type-A receptor 3
    EC=2.7.10.1
Alternative name(s):
    Tyrosine-protein kinase receptor ZEK1
    EPH-like kinase 1

Gene names

Name:	epha3
Synonyms:	ek1, zek1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	981 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Receptor for members of the ephrin-B family. May play a role in early pattern formation within the developing nervous system.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Widely expressed in the developing zebrafish nervous system.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Signal Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Transferase Tyrosine-protein kinase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro transmembrane receptor protein tyrosine kinase signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ephrin receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Chain	21 – 981	961	Ephrin type-A receptor 3		PRO_0000016806
Regions
	Topological domain	21 – 545	525	Extracellular Potential
	Transmembrane	546 – 566	21	Potential
	Topological domain	567 – 981	415	Cytoplasmic Potential
	Domain	328 – 433	106	Fibronectin type-III 1
	Domain	437 – 530	94	Fibronectin type-III 2
	Domain	626 – 887	262	Protein kinase
	Domain	910 – 974	65	SAM
	Nucleotide binding	632 – 640	9	ATP By similarity
	Motif	979 – 981	3	PDZ-binding Potential
	Compositional bias	192 – 325	134	Cys-rich
Sites
	Active site	751	1	Proton acceptor By similarity
	Binding site	658	1	ATP By similarity
Amino acid modifications
	Modified residue	601	1	Phosphotyrosine; by autocatalysis Potential
	Modified residue	607	1	Phosphotyrosine; by autocatalysis Potential
	Modified residue	784	1	Phosphotyrosine; by autocatalysis Potential
	Modified residue	927	1	Phosphotyrosine; by autocatalysis Potential
	Glycosylation	340	1	N-linked (GlcNAc...) Potential
	Glycosylation	410	1	N-linked (GlcNAc...) Potential
	Glycosylation	435	1	N-linked (GlcNAc...) Potential
	Glycosylation	485	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	141	1	S → N

Sequences

	Sequence		Length	Mass (Da)
	O13146-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: F0B3F5218965E2C6	FASTA	981	109,655
	10 20 30 40 50 60 MALFRIYSFL APFHILVLCQ ALRNYPDNEV TLLDSMSAPG DLGWEAYPSE GWEEISVMDE 70 80 90 100 110 120 RNIPMRTYQV CNVMEANQNN WLRTGLIQRE GAQRVYVEIK FTLRDCNSLP GVPGTCKETF 130 140 150 160 170 180 NVYYHESNNA VAAPLRHIRE SQYIKIDTIA ADESFTQTDV GDRVMKLNTE VRDISGLSKR 190 200 210 220 230 240 GLYLAFQDLG ACIALVSVRV FYKRCPLAVL NLARFPDTVT GGDSALVEVR GTCVEDAEEL 250 260 270 280 290 300 EGPRMFCSAD GGWLVPIGRC VCRPGFEEVD GHCQPCRSGF YKASAMDAYC VKCPPHSYSH 310 320 330 340 350 360 QDKASECVCE RGFYRAESDP RSMACTRPPS APGNPISMVN ETAVTLEWSP PRDSGGRGDV 370 380 390 400 410 420 SYSVHCRKCS GETGASERCV PCGSGAHFNP RQFGLTHPRV LVTELQPHTN YTFSVEALNG 430 440 450 460 470 480 VSDLSPSPRQ LVSVNVTTSQ TVSVILKERK GTDSVTLAWQ GPEPVDGTVV EYEVTYYEKN 490 500 510 520 530 540 QQDQNYTVLK TKSNSMTVDG LKPGTTYIFR VRARTDGGYG NYKGEIELET SHEDMLAVGD 550 560 570 580 590 600 PNQQTILAIS VAGGAVLLVL LVACFIVSGR RCGYIKAKQD PEEEKMQFQH GRVKLPETRT 610 620 630 640 650 660 YIHPHTYEDP NQAVRDFAKE IEVSNIRIER VIGAGEFGEV CSGRLRLPSK REIQVAIKSL 670 680 690 700 710 720 KAGYSEHQRR DFLSEASIMG QFDHPNIIRL EGVVTRCKPV MIVTEYMENG SLDTFLKKHD 730 740 750 760 770 780 GQFTVIQLLG MLRGIAAGMQ YLSEMNYVHR DLAARNILVN RNLVCKVSDF GLSRVLEDDP 790 800 810 820 830 840 EAAYTTRGGK IPIRWTAPEA ITYRKFTSAS DVWSYGIVMW EVISYGERPY WEMSNQDVIK 850 860 870 880 890 900 AVDEGYRLPA PMDCPVVLHQ LMLDCWEKNR SDRPKFGQIV NTLDRLIRNP SSLKQLANSA 910 920 930 940 950 960 VWEDPVTPEA AVNTVEDWLD LIKMGQYKEH FSSAGYVTLD SVLYVSSSEL DKMGVELAGH 970 980 QKKILSSIQC LQAHHGTQVQ V

References

[1]	"Novel Eph-family receptor tyrosine kinase is widely expressed in the developing zebrafish nervous system." Bovenkamp D.E., Greer P. Dev. Dyn. 209:166-181(1997) [PubMed: 9186052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U89295 mRNA. Translation: AAC60220.1.
IPI	IPI00497981.
RefSeq	NP_571170.1.
UniGene	Dr.75760
3D structure databases
HSSP	HSSP built from PDB template 1JPA based on UniProtKB P54763.
SMR	O13146. Positions 606-904.
ModBase	Search...
Genome annotation databases
Ensembl	ENSDART00000105774; ENSDARP00000096552; ENSDARG00000020080; Danio rerio. [Genome view]
GeneID	30311.
Organism-specific databases
CTD	30311.
ZFIN	ZDB-GENE-990415-58. ek1.
Phylogenomic databases
HOVERGEN	O13146.
Gene expression databases
ArrayExpress	O13146.
Bgee	O13146.
Family and domain databases
InterPro	IPR013032. EGF-like_reg_CS. IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR011510. SAM_2. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR016257. TyrPK_ephrin_receptor. IPR001426. YKase_receptorV_CS. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 2 hits.
Pfam	PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF07714. Pkinase_Tyr. 1 hit. PF07647. SAM_2. 1 hit. [Graphical view]
PIRSF	PIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTS	PR00014. FNTYPEIII. PR00109. TYRKINASE.
ProDom	PD001495. Ephrin_receptor. 1 hit. PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS01186. EGF_2. 1 hit. Uncertain. PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EPHA3_DANRE

Accession

Primary (citable) accession number: O13146

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 1, 1997
Last modified:	October 13, 2009
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project