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O13134 (S4A4_AMBTI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Electrogenic sodium bicarbonate cotransporter 1

Short name=Sodium bicarbonate cotransporter
Alternative name(s):
Na(+)/HCO3(-) cotransporter
Solute carrier family 4 member 4
Gene names
Name:SLC4A4
Synonyms:NBC, NBCE1
OrganismAmbystoma tigrinum (Eastern tiger salamander)
Taxonomic identifier8305 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaCaudataSalamandroideaAmbystomatidaeAmbystoma

Protein attributes

Sequence length1035 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Electrogenic sodium/bicarbonate cotransporter with a Na+:HCO3- stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. Ref.1

Subcellular location

Basolateral cell membrane; Multi-pass membrane protein Ref.2.

Tissue specificity

Expressed in kidney and to a lower extent in bladder, brain, intestine, large intestine and eye. Ref.1

Sequence similarities

Belongs to the anion exchanger (TC 2.A.31) family. [View classification]

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10351035Electrogenic sodium bicarbonate cotransporter 1
PRO_0000079232

Regions

Topological domain1 – 426426Cytoplasmic Potential
Transmembrane427 – 44721Helical; Potential
Topological domain448 – 4547Extracellular Potential
Transmembrane455 – 47521Helical; Potential
Topological domain476 – 51136Cytoplasmic Potential
Transmembrane512 – 53221Helical; Potential
Topological domain533 – 644112Extracellular Potential
Transmembrane645 – 66521Helical; Potential
Topological domain666 – 68116Cytoplasmic Potential
Transmembrane682 – 70221Helical; Potential
Topological domain703 – 73230Extracellular Potential
Transmembrane733 – 75321Helical; Potential
Topological domain754 – 77825Cytoplasmic Potential
Transmembrane779 – 79921Helical; Potential
Topological domain800 – 83738Extracellular Potential
Transmembrane838 – 85821Helical; Potential
Transmembrane859 – 87921Helical; Potential
Topological domain880 – 91940Extracellular Potential
Transmembrane920 – 94021Helical; Potential
Topological domain941 – 103595Cytoplasmic Potential
Compositional bias965 – 98016Lys-rich

Amino acid modifications

Glycosylation5911N-linked (GlcNAc...) Potential
Glycosylation5961N-linked (GlcNAc...) Potential
Glycosylation6091N-linked (GlcNAc...) Potential
Glycosylation6171N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O13134 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 3CF50B31275A81AF

FASTA1,035116,108
        10         20         30         40         50         60 
MSSEKECLEN MLNGYAESGR VLSRTSLVIN QAVNRSIFTS TVSPAAERIR FILGEEDDSP 

        70         80         90        100        110        120 
APPQLFTELD ELLAVDGQEM EWKETARWIK FEEKVEQGGE RWSKPHVATL SLHSLFELRT 

       130        140        150        160        170        180 
CIEKGTILLD LEATSLPQIV EIVINNQIEL GLLKADMKEN VTRTLLRKHR HQTKKSNLRS 

       190        200        210        220        230        240 
LADIGKTVSS ASRLFSTPDN GSPTMTHRNL TSTSLNDVSD KPDKEQLKNK FMKKLPRDAE 

       250        260        270        280        290        300 
ASNVLVGEVD FLESPFIAFV RLQQAVMLGS LTEVPVPTRF LFILLGPKGK AKSYHEIGRS 

       310        320        330        340        350        360 
IATLMSDEVF HDIAYKAKNR EDLIAGIDEF LDEVIVLPLG EWDPTIRIEP PKSLPSSDKR 

       370        380        390        400        410        420 
KNMYSGGDNL QMNGDAPHDD GGGGHGDSEE LQRTGRFCGG LIKDIQRKAP FFASDFYDAL 

       430        440        450        460        470        480 
SIQSLSAILF IYLGTVTNAI TFGGLLGDAT ENMQGVLESF LGTAVSGAVF CLFGGQPLTI 

       490        500        510        520        530        540 
LSSTGPVLVF ERLLFNFSKD NDFDYLEFRL WIGLWSAFQC LILVATDASF LVKYFTRFTE 

       550        560        570        580        590        600 
EGFSSLISFI FIYDAFKKMI KLADYYPINS HFKVDYITQY SCACFPPEPA NSSWFNMTTA 

       610        620        630        640        650        660 
ATTTQFLTNA STDMAYNGTI DWSLLSKKEC LKYGGLLVGS NCKYVPDITL MSFILFLGTY 

       670        680        690        700        710        720 
TCSMALKKFK TSRYFPTTAR KLISDFAIIL SILIFCGLDA LLGVDTPKLI VPSEFKPTSP 

       730        740        750        760        770        780 
NRGWFVPPFG GNPWWVYLAA AIPALLVTIL IFMDQQITGV IVNRKEHKLK KGAGYHLDLF 

       790        800        810        820        830        840 
WVAILMVVCS FMALPWYVAA TVISIAHIDS LKMETETSAP GEQPKFLGVR EQRVTGTVVF 

       850        860        870        880        890        900 
LLTGLSVFMA PILKFIPMPV LYGVFLYMGV ASLNGVQFMD RLKLLLMPPK YQPDFIYLRH 

       910        920        930        940        950        960 
VPLRRVHLFT FLQVVCLAML WILKSTVAAI IFPVMILALV AVRKAMDYFF SQHDLSFLDD 

       970        980        990       1000       1010       1020 
VIPEKDKKKK EDEKKKKKKK GSIDSDVEDS DCPYPEKVPS IKIPMDIMEK EPFLIDSKPS 

      1030 
DRENSPTFLE RHTSC 

« Hide

References

[1]"Expression cloning and characterization of a renal electrogenic Na+/HCO3- cotransporter."
Romero M.F., Hediger M.A., Boulpaep E.L., Boron W.F.
Nature 387:409-413(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Tissue: Kidney.
[2]"Immunoelectron microscopic localization of the electrogenic Na/HCO(3) cotransporter in rat and ambystoma kidney."
Maunsbach A.B., Vorum H., Kwon T.-H., Nielsen S., Simonsen B., Choi I., Schmitt B.M., Boron W.F., Aalkjaer C.
J. Am. Soc. Nephrol. 11:2179-2189(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF001958 mRNA. Translation: AAB61339.1.
PIRT31336.

3D structure databases

ProteinModelPortalO13134.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG004326.

Family and domain databases

Gene3D3.40.1100.10. 1 hit.
InterProIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERPTHR11453. PTHR11453. 1 hit.
PfamPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMSSF55804. SSF55804. 1 hit.
TIGRFAMsTIGR00834. ae. 1 hit.
ProtoNetSearch...

Entry information

Entry nameS4A4_AMBTI
AccessionPrimary (citable) accession number: O13134
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families