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Protein

Electrogenic sodium bicarbonate cotransporter 1

Gene

SLC4A4

Organism
Ambystoma tigrinum (Eastern tiger salamander)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Electrogenic sodium/bicarbonate cotransporter with a Na+:HCO3- stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Electrogenic sodium bicarbonate cotransporter 1
Short name:
Sodium bicarbonate cotransporter
Alternative name(s):
Na(+)/HCO3(-) cotransporter
Solute carrier family 4 member 4
Gene namesi
Name:SLC4A4
Synonyms:NBC, NBCE1
OrganismiAmbystoma tigrinum (Eastern tiger salamander)
Taxonomic identifieri8305 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaCaudataSalamandroideaAmbystomatidaeAmbystoma

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 426CytoplasmicSequence analysisAdd BLAST426
Transmembranei427 – 447HelicalSequence analysisAdd BLAST21
Topological domaini448 – 454ExtracellularSequence analysis7
Transmembranei455 – 475HelicalSequence analysisAdd BLAST21
Topological domaini476 – 511CytoplasmicSequence analysisAdd BLAST36
Transmembranei512 – 532HelicalSequence analysisAdd BLAST21
Topological domaini533 – 644ExtracellularSequence analysisAdd BLAST112
Transmembranei645 – 665HelicalSequence analysisAdd BLAST21
Topological domaini666 – 681CytoplasmicSequence analysisAdd BLAST16
Transmembranei682 – 702HelicalSequence analysisAdd BLAST21
Topological domaini703 – 732ExtracellularSequence analysisAdd BLAST30
Transmembranei733 – 753HelicalSequence analysisAdd BLAST21
Topological domaini754 – 778CytoplasmicSequence analysisAdd BLAST25
Transmembranei779 – 799HelicalSequence analysisAdd BLAST21
Topological domaini800 – 837ExtracellularSequence analysisAdd BLAST38
Transmembranei838 – 858HelicalSequence analysisAdd BLAST21
Transmembranei859 – 879HelicalSequence analysisAdd BLAST21
Topological domaini880 – 919ExtracellularSequence analysisAdd BLAST40
Transmembranei920 – 940HelicalSequence analysisAdd BLAST21
Topological domaini941 – 1035CytoplasmicSequence analysisAdd BLAST95

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000792321 – 1035Electrogenic sodium bicarbonate cotransporter 1Add BLAST1035

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi582 ↔ 584By similarity
Glycosylationi591N-linked (GlcNAc...)Sequence analysis1
Glycosylationi596N-linked (GlcNAc...)Sequence analysis1
Glycosylationi609N-linked (GlcNAc...)Sequence analysis1
Glycosylationi617N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi630 ↔ 642By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed in kidney and to a lower extent in bladder, brain, intestine, large intestine and eye.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO13134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi965 – 980Lys-richAdd BLAST16

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG004326.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 2 hits.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.

Sequencei

Sequence statusi: Complete.

O13134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEKECLEN MLNGYAESGR VLSRTSLVIN QAVNRSIFTS TVSPAAERIR
60 70 80 90 100
FILGEEDDSP APPQLFTELD ELLAVDGQEM EWKETARWIK FEEKVEQGGE
110 120 130 140 150
RWSKPHVATL SLHSLFELRT CIEKGTILLD LEATSLPQIV EIVINNQIEL
160 170 180 190 200
GLLKADMKEN VTRTLLRKHR HQTKKSNLRS LADIGKTVSS ASRLFSTPDN
210 220 230 240 250
GSPTMTHRNL TSTSLNDVSD KPDKEQLKNK FMKKLPRDAE ASNVLVGEVD
260 270 280 290 300
FLESPFIAFV RLQQAVMLGS LTEVPVPTRF LFILLGPKGK AKSYHEIGRS
310 320 330 340 350
IATLMSDEVF HDIAYKAKNR EDLIAGIDEF LDEVIVLPLG EWDPTIRIEP
360 370 380 390 400
PKSLPSSDKR KNMYSGGDNL QMNGDAPHDD GGGGHGDSEE LQRTGRFCGG
410 420 430 440 450
LIKDIQRKAP FFASDFYDAL SIQSLSAILF IYLGTVTNAI TFGGLLGDAT
460 470 480 490 500
ENMQGVLESF LGTAVSGAVF CLFGGQPLTI LSSTGPVLVF ERLLFNFSKD
510 520 530 540 550
NDFDYLEFRL WIGLWSAFQC LILVATDASF LVKYFTRFTE EGFSSLISFI
560 570 580 590 600
FIYDAFKKMI KLADYYPINS HFKVDYITQY SCACFPPEPA NSSWFNMTTA
610 620 630 640 650
ATTTQFLTNA STDMAYNGTI DWSLLSKKEC LKYGGLLVGS NCKYVPDITL
660 670 680 690 700
MSFILFLGTY TCSMALKKFK TSRYFPTTAR KLISDFAIIL SILIFCGLDA
710 720 730 740 750
LLGVDTPKLI VPSEFKPTSP NRGWFVPPFG GNPWWVYLAA AIPALLVTIL
760 770 780 790 800
IFMDQQITGV IVNRKEHKLK KGAGYHLDLF WVAILMVVCS FMALPWYVAA
810 820 830 840 850
TVISIAHIDS LKMETETSAP GEQPKFLGVR EQRVTGTVVF LLTGLSVFMA
860 870 880 890 900
PILKFIPMPV LYGVFLYMGV ASLNGVQFMD RLKLLLMPPK YQPDFIYLRH
910 920 930 940 950
VPLRRVHLFT FLQVVCLAML WILKSTVAAI IFPVMILALV AVRKAMDYFF
960 970 980 990 1000
SQHDLSFLDD VIPEKDKKKK EDEKKKKKKK GSIDSDVEDS DCPYPEKVPS
1010 1020 1030
IKIPMDIMEK EPFLIDSKPS DRENSPTFLE RHTSC
Length:1,035
Mass (Da):116,108
Last modified:July 1, 1997 - v1
Checksum:i3CF50B31275A81AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001958 mRNA. Translation: AAB61339.1.
PIRiT31336.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF001958 mRNA. Translation: AAB61339.1.
PIRiT31336.

3D structure databases

ProteinModelPortaliO13134.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG004326.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR003024. Na/HCO3_transpt.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 2 hits.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 1 hit.
[Graphical view]
PRINTSiPR01231. HCO3TRNSPORT.
PR01232. NAHCO3TRSPRT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiS4A4_AMBTI
AccessioniPrimary (citable) accession number: O13134
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 1, 1997
Last modified: November 11, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.