O13113 (DAD1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Short name=Oligosaccharyl transferase subunit DAD1
EC=2.4.1.119

Alternative name(s):
Defender against cell death 1
Short name=DAD-1

Gene names

Name:

DAD1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	123 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the DAD/OST2 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 123	123	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1		PRO_0000124015
Regions
	Transmembrane	41 – 61	21	Helical; Potential
	Transmembrane	63 – 83	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	O13113 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: D468651E6A5D01C5	FASTA	123	12,926
	10 20 30 40 50 60 MSGTAGSGVG AAGSVGSVVR RFLAEYGSGT SSRLKVLDAY LLYVMLTGAL QFGYCLGVGT 70 80 90 100 110 120 FPFNSFLSGF ISAVGSFILG VCLRIQINPQ NKGEFQGISP ERAFADFLFA NTILHLVVIN FVG

References

[1]	"A highly conserved apoptotic suppressor gene is located near the chicken T-cell receptor alpha chain constant region." Wang K., Gan L., Kuo C.L., Hood L. Immunogenetics 46:376-382(1997) [PubMed: 9271627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U83627 mRNA. Translation: AAC60276.1.
IPI	IPI00575128.
RefSeq	NP_001007474.1. NM_001007473.1.
UniGene	Gga.4960.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O13113.
Proteomic databases
PRIDE	O13113.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000000262; ENSGALP00000000261; ENSGALG00000000196.
GeneID	395343.
KEGG	gga:395343.
Organism-specific databases
CTD	1603.
Phylogenomic databases
HOGENOM	HBG329225.
HOVERGEN	HBG004452.
InParanoid	O13113.
OrthoDB	EOG4G7C0W.
PhylomeDB	O13113.
Family and domain databases
InterPro	IPR003038. DAD. [Graphical view]
KO	K12668.
PANTHER	PTHR10705. DAD. 1 hit.
Pfam	PF02109. DAD. 1 hit. [Graphical view]
PIRSF	PIRSF005588. DAD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DAD1_CHICK

Accession

Primary (citable) accession number: O13113

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	July 1, 1997
Last modified:	November 16, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program