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Protein

Ephrin-B1

Gene

efnb1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling (By similarity). May have a role in the developing mesenchymal and nervous tissue.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin-B1
Alternative name(s):
ELK ligand
Short name:
ELK-L
EPH-related receptor tyrosine kinase ligand 2
Short name:
LERK-2
XlERK
Gene namesi
Name:efnb1
Synonyms:eplg2, lerk2
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-1011784. efnb1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 226206ExtracellularSequence analysisAdd
BLAST
Transmembranei227 – 24721HelicalSequence analysisAdd
BLAST
Topological domaini248 – 32982CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 329309Ephrin-B1PRO_0000008391Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi57 ↔ 94PROSITE-ProRule annotation
Disulfide bondi82 ↔ 146PROSITE-ProRule annotation
Glycosylationi132 – 1321N-linked (GlcNAc...)Sequence analysis
Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. Tyrosine phosphorylation inhibits TLE4-binding.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Expressioni

Tissue specificityi

Expressed at low levels in most tissues with highest levels in the kidney, oocytes, ovary and testis.

Developmental stagei

At stages 16 and 32, expressed in the neural groove, rhombomeres, forebrain and branchial arches.1 Publication

Interactioni

Subunit structurei

Interacts with TLE4 through the PDZ-binding motif.1 Publication

Protein-protein interaction databases

DIPiDIP-60983N.

Structurei

3D structure databases

ProteinModelPortaliO13097.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 157135Ephrin RBDPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi327 – 3293PDZ-bindingSequence analysis

Domaini

The PDZ-binding motif is required for TLE4-binding.1 Publication

Sequence similaritiesi

Belongs to the ephrin family.PROSITE-ProRule annotation
Contains 1 ephrin RBD (ephrin receptor-binding) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG051448.
KOiK05463.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR008972. Cupredoxin.
IPR031328. Ephrin.
IPR019765. Ephrin_CS.
IPR001799. Ephrin_RBD.
[Graphical view]
PANTHERiPTHR11304. PTHR11304. 1 hit.
PfamiPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSiPR01347. EPHRIN.
ProDomiPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O13097-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGLRRLLGL LLVLYRLCSA LGKNLEPVTW NSQNPRFISG KGLVLYPEIG
60 70 80 90 100
DRLDIICPKG DSSQPYEYYK LYMVRRDQLE ACSTVIDPNV LVTCNQPGKE
110 120 130 140 150
YRFTIKFQEF SPNYMGLEFR RNQDYYITST SNSTLQGLEN REGGVCQTRS
160 170 180 190 200
MKIIMKVGQD PNAVPPEQLT TTRPSKEADN TGKIATFGPW NGPVENPGKS
210 220 230 240 250
DTNLSDKPTA GGGVDGFFNS KIAVFAAIGA GCVIFILIII FLVVLLIKIR
260 270 280 290 300
KRHRKHTQQR AAALSLSTLA SPKCSGNAGS EPSDIIIPLR TTENNYCPHY
310 320
EKVSGDYGHP VYIVQEMPPQ SPANIYYKV
Length:329
Mass (Da):36,593
Last modified:December 16, 2008 - v2
Checksum:iD1D6721FF0A2AF1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31427 mRNA. Translation: AAC35995.2.
RefSeqiNP_001080948.2. NM_001087479.2.
UniGeneiXl.302.

Genome annotation databases

GeneIDi394292.
KEGGixla:394292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31427 mRNA. Translation: AAC35995.2.
RefSeqiNP_001080948.2. NM_001087479.2.
UniGeneiXl.302.

3D structure databases

ProteinModelPortaliO13097.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60983N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi394292.
KEGGixla:394292.

Organism-specific databases

CTDi1947.
XenbaseiXB-GENE-1011784. efnb1.

Phylogenomic databases

HOVERGENiHBG051448.
KOiK05463.

Family and domain databases

Gene3Di2.60.40.420. 1 hit.
InterProiIPR008972. Cupredoxin.
IPR031328. Ephrin.
IPR019765. Ephrin_CS.
IPR001799. Ephrin_RBD.
[Graphical view]
PANTHERiPTHR11304. PTHR11304. 1 hit.
PfamiPF00812. Ephrin. 1 hit.
[Graphical view]
PRINTSiPR01347. EPHRIN.
ProDomiPD002533. Ephrin. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF49503. SSF49503. 1 hit.
PROSITEiPS01299. EPHRIN_RBD_1. 1 hit.
PS51551. EPHRIN_RBD_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification of XLerk, an Eph family ligand regulated during mesoderm induction and neurogenesis in Xenopus laevis."
    Jones T.L., Karavanova I., Chong L., Zhou R.P., Daar I.O.
    Oncogene 14:2159-2166(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Daar I.O.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 61-63; 195; 210-212 AND 260.
  3. "EphrinB1 interacts with the transcriptional co-repressor Groucho/xTLE4."
    Kamata T., Bong Y.S., Mood K., Park M.J., Nishanian T.G., Lee H.S.
    BMB Rep. 44:199-204(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TLE4, DEVELOPMENTAL STAGE, PHOSPHORYLATION.

Entry informationi

Entry nameiEFNB1_XENLA
AccessioniPrimary (citable) accession number: O13097
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 16, 2008
Last modified: November 11, 2015
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.