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Protein

Thrombin-like enzyme KN-BJ 2

Gene
N/A
Organism
Bothrops jararaca (Jararaca) (Bothrops jajaraca)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Thrombin-like snake venom serine protease that clots fibrinogen (FGA) by releasing only fibrinopeptide A. Also releases bradykinin from bovine low-molecular-mass kininogen (KNG).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei64Charge relay systemBy similarity1
Active sitei109Charge relay systemBy similarity1
Active sitei203Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Blood coagulation cascade activating toxin, Hemostasis impairing toxin, Hydrolase, Hypotensive agent, Protease, Serine protease, Toxin

Protein family/group databases

MEROPSiS01.354.

Names & Taxonomyi

Protein namesi
Recommended name:
Thrombin-like enzyme KN-BJ 2 (EC:3.4.21.-)
Short name:
SVTLE
Alternative name(s):
Kinin-releasing and fibrinogen-clotting serine protease 2
OrganismiBothrops jararaca (Jararaca) (Bothrops jajaraca)
Taxonomic identifieri8724 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeBothrops

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
PropeptideiPRO_000002838119 – 246
ChainiPRO_000002838225 – 257Thrombin-like enzyme KN-BJ 2Add BLAST233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 162PROSITE-ProRule annotation
Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
Disulfide bondi97 ↔ 255PROSITE-ProRule annotation
Glycosylationi102N-linked (GlcNAc...)1 Publication1
Disulfide bondi141 ↔ 209PROSITE-ProRule annotation
Glycosylationi169N-linked (GlcNAc...)1 Publication1
Disulfide bondi173 ↔ 188PROSITE-ProRule annotation
Disulfide bondi199 ↔ 224PROSITE-ProRule annotation
Glycosylationi250N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Expressioni

Tissue specificityi

Expressed by the venom gland.

Developmental stagei

This protein seems to be only found in adult B.jararaca venom (and not in newborn snakes).1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO13069.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 248Peptidase S1PROSITE-ProRule annotationAdd BLAST224

Sequence similaritiesi

Belongs to the peptidase S1 family. Snake venom subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O13069-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLIRVLANL LILQLSYAQK ASELIIGGRP CDINEHRSLA LVKYGNFQCS
60 70 80 90 100
GTLINQEWVL SAAHCDGEKM KIHLGVHSKK VPNKDKQTRV AKEKFFCLSS
110 120 130 140 150
KNYTKWDKDI MLIRLDSPVK NSAHIAPISL PSSPPIVGSV CRIMGWGTIS
160 170 180 190 200
TSKVILSDVP HCANINLLNY TVCRAAYPEL PATSRTLCAG ILQGGKDTCV
210 220 230 240 250
GDSGGPLICN GQFQGIVSWG SDVCGYVLEP ALYTKVSDYT EWINSIIAGN

TTATCPP
Length:257
Mass (Da):27,894
Last modified:July 1, 1997 - v1
Checksum:iA9F96BFA6BDDDDE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004067 mRNA. Translation: BAA20283.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004067 mRNA. Translation: BAA20283.1.

3D structure databases

ProteinModelPortaliO13069.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.354.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG013304.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVSP2_BOTJA
AccessioniPrimary (citable) accession number: O13069
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: July 1, 1997
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.