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Protein

Matrix metalloproteinase-18

Gene

mmp18

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves collagen type I. May play a role in larval tissue degeneration and adult organogenesis during amphibian metamorphosis. May be involved in tail resorption.

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
  • Ca2+By similarity

Enzyme regulationi

Up-regulated in the tail by thyroid hormone.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921Zinc; in inhibited formBy similarity
Metal bindingi218 – 2181Zinc; catalyticPROSITE-ProRule annotation
Active sitei219 – 2191PROSITE-ProRule annotation
Metal bindingi222 – 2221Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi228 – 2281Zinc; catalyticPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Collagen degradation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM10.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-18 (EC:3.4.24.-)
Short name:
MMP-18
Alternative name(s):
Collagenase-4
Short name:
xCol4
Gene namesi
Name:mmp18
Synonyms:col4
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence analysisAdd
BLAST
Propeptidei18 – 9982By similarityPRO_0000028824Add
BLAST
Chaini100 – 467368Matrix metalloproteinase-18PRO_0000028825Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi280 ↔ 467Sequence analysis

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PRIDEiO13065.

Expressioni

Tissue specificityi

Expressed only transiently in whole animal, at time when tadpole feeding begins.

Developmental stagei

Expressed at high level as the tadpole tail resorbs and during hindlimb morphogenesis and intestinal remodeling.

Structurei

3D structure databases

ProteinModelPortaliO13065.
SMRiO13065. Positions 109-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati277 – 32650Hemopexin 1Add
BLAST
Repeati327 – 37347Hemopexin 2Add
BLAST
Repeati375 – 42349Hemopexin 3Add
BLAST
Repeati424 – 46744Hemopexin 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi90 – 978Cysteine switchBy similarity

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOVERGENiHBG052484.

Family and domain databases

Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF00045. Hemopexin. 3 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O13065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSLLLKLLL CVAITAAFPA DKQDEPPATK EEMAENYLKR FYSLGTDGGP
60 70 80 90 100
VGRKKHIQPF TEKLEQMQKF FGLKVTGTLD PKTVEVMEKP RCGVYDVGQY
110 120 130 140 150
STVAKSSAWQ KKDLTYRILN FTPDLPQADV ETAIQRAFKV WSDVTPLTFT
160 170 180 190 200
RIYNEVSDIE ISFTAGDHKD NSPFDGSGGI LAHAFQPGNG IGGDAHFDED
210 220 230 240 250
ETWTKTSEIY NLFLVAAHEF GHSLGLSHST DQGALMYPTY SNTDPKTFQL
260 270 280 290 300
PQDDINAIQY LYGKSSNPVQ PTGPSTPSRC DPNVVFNAVT TMRGELIFFV
310 320 330 340 350
KRFLWRKHPQ ASEAELMFVQ AFWPSLPTNI DAAYENPITE QILVFKGSKY
360 370 380 390 400
TALDGFDVVQ GYPRNIYSLG FPKTVKRIDA AVHIEQLGKT YFFAAKKYWS
410 420 430 440 450
YDEDKKQMDK GFPKQISNDF PGIPDKIDAA FYYRGRLYFF IGRSQFEYNI
460
NSKRIVQVLR SNSWLGC
Length:467
Mass (Da):52,813
Last modified:July 1, 1997 - v1
Checksum:i4623F6CEF3454051
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76275 Genomic DNA. Translation: AAB53148.1.
UniGeneiXl.81375.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76275 Genomic DNA. Translation: AAB53148.1.
UniGeneiXl.81375.

3D structure databases

ProteinModelPortaliO13065.
SMRiO13065. Positions 109-271.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM10.018.

Proteomic databases

PRIDEiO13065.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG052484.

Family and domain databases

Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF00045. Hemopexin. 3 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification and characterization of a novel collagenase in Xenopus laevis: possible roles during frog development."
    Stolow M.A., Bauzon D.D., Li J., Sedgwick T., Liang V.C.-T., Sang Q.A., Shi Y.-B.
    Mol. Biol. Cell 7:1471-1483(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Intestine.

Entry informationi

Entry nameiMMP18_XENLA
AccessioniPrimary (citable) accession number: O13065
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 1, 1997
Last modified: April 13, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.