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Protein

Matrix metalloproteinase-18

Gene

mmp18

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves collagen type I. May play a role in larval tissue degeneration and adult organogenesis during amphibian metamorphosis. May be involved in tail resorption.

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
  • Ca2+By similarity

Enzyme regulationi

Up-regulated in the tail by thyroid hormone.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi92Zinc; in inhibited formBy similarity1
Metal bindingi218Zinc; catalyticPROSITE-ProRule annotation1
Active sitei219PROSITE-ProRule annotation1
Metal bindingi222Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi228Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Biological processi

Collagen degradation

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM10.018.

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix metalloproteinase-18 (EC:3.4.24.-)
Short name:
MMP-18
Alternative name(s):
Collagenase-4
Short name:
xCol4
Gene namesi
Name:mmp18
Synonyms:col4
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000002882418 – 99By similarityAdd BLAST82
ChainiPRO_0000028825100 – 467Matrix metalloproteinase-18Add BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi280 ↔ 467Sequence analysis

Keywords - PTMi

Disulfide bond, Zymogen

Expressioni

Tissue specificityi

Expressed only transiently in whole animal, at time when tadpole feeding begins.

Developmental stagei

Expressed at high level as the tadpole tail resorbs and during hindlimb morphogenesis and intestinal remodeling.

Structurei

3D structure databases

ProteinModelPortaliO13065.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati277 – 326Hemopexin 1Add BLAST50
Repeati327 – 373Hemopexin 2Add BLAST47
Repeati375 – 423Hemopexin 3Add BLAST49
Repeati424 – 467Hemopexin 4Add BLAST44

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi90 – 97Cysteine switchBy similarity8

Domaini

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Sequence similaritiesi

Belongs to the peptidase M10A family.Curated
Contains 4 hemopexin repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOVERGENiHBG052484.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF00045. Hemopexin. 3 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O13065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSLLLKLLL CVAITAAFPA DKQDEPPATK EEMAENYLKR FYSLGTDGGP
60 70 80 90 100
VGRKKHIQPF TEKLEQMQKF FGLKVTGTLD PKTVEVMEKP RCGVYDVGQY
110 120 130 140 150
STVAKSSAWQ KKDLTYRILN FTPDLPQADV ETAIQRAFKV WSDVTPLTFT
160 170 180 190 200
RIYNEVSDIE ISFTAGDHKD NSPFDGSGGI LAHAFQPGNG IGGDAHFDED
210 220 230 240 250
ETWTKTSEIY NLFLVAAHEF GHSLGLSHST DQGALMYPTY SNTDPKTFQL
260 270 280 290 300
PQDDINAIQY LYGKSSNPVQ PTGPSTPSRC DPNVVFNAVT TMRGELIFFV
310 320 330 340 350
KRFLWRKHPQ ASEAELMFVQ AFWPSLPTNI DAAYENPITE QILVFKGSKY
360 370 380 390 400
TALDGFDVVQ GYPRNIYSLG FPKTVKRIDA AVHIEQLGKT YFFAAKKYWS
410 420 430 440 450
YDEDKKQMDK GFPKQISNDF PGIPDKIDAA FYYRGRLYFF IGRSQFEYNI
460
NSKRIVQVLR SNSWLGC
Length:467
Mass (Da):52,813
Last modified:July 1, 1997 - v1
Checksum:i4623F6CEF3454051
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76275 Genomic DNA. Translation: AAB53148.1.
UniGeneiXl.81375.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L76275 Genomic DNA. Translation: AAB53148.1.
UniGeneiXl.81375.

3D structure databases

ProteinModelPortaliO13065.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM10.018.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG052484.

Family and domain databases

CDDicd00094. HX. 1 hit.
cd04278. ZnMc_MMP. 1 hit.
Gene3Di2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProiIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR033739. M10A_MMP.
IPR024079. MetalloPept_cat_dom.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR016293. Pept_M10A_stromelysin-type.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PfamiPF00045. Hemopexin. 3 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSiPR00138. MATRIXIN.
SMARTiSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMiSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEiPS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMMP18_XENLA
AccessioniPrimary (citable) accession number: O13065
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.