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O12990

- JAK1_DANRE

UniProt

O12990 - JAK1_DANRE

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Protein
Tyrosine-protein kinase JAK1
Gene
jak1
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei905 – 9051ATP
Active sitei1000 – 10001Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi878 – 8869ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase JAK1 (EC:2.7.10.2)
Alternative name(s):
Janus kinase 1
Short name:
JAK-1
Gene namesi
Name:jak1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-980526-142. jak1.

Subcellular locationi

Endomembrane system; Peripheral membrane protein By similarity
Note: Wholly intracellular, possibly membrane associated By similarity.

GO - Cellular componenti

  1. cytoskeleton Source: InterPro
  2. endomembrane system Source: UniProtKB-SubCell
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi905 – 9051K → E: Loss of autophosphorylation and defects in early development. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11531153Tyrosine-protein kinase JAK1
PRO_0000088110Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1031 – 10311Phosphotyrosine; by autocatalysis By similarity
Modified residuei1032 – 10321Phosphotyrosine; by autocatalysis By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO12990.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 416385FERM
Add
BLAST
Domaini435 – 540106SH2; atypical
Add
BLAST
Domaini580 – 846267Protein kinase 1
Add
BLAST
Domaini872 – 1150279Protein kinase 2
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi336 – 35621Lys-rich (basic)
Add
BLAST

Domaini

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

Sequence similaritiesi

Contains 1 FERM domain.
Contains 1 SH2 domain.

Keywords - Domaini

Repeat, SH2 domain

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000049158.
HOVERGENiHBG006195.
InParanoidiO12990.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020776. Tyr_kinase_non-rcpt_Jak1.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 2 hits.
[Graphical view]
PIRSFiPIRSF000636. TyrPK_Jak. 1 hit.
PRINTSiPR01823. JANUSKINASE.
PR01824. JANUSKINASE1.
PR00109. TYRKINASE.
SMARTiSM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O12990-1 [UniParc]FASTAAdd to Basket

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MPELAVMDLG RQLCVKMKKQ RKAEMTIPTA MKGLEIHFYL ADTHQLEFFK     50
ACYTAEDLCV EAAKRCRISP LCHNLFALYE ESQDLWYAPN HVFKVTDETS 100
IKLHYRMRFY FTNWHGTSEI ESPVWRHTLS KQKSVLNSQK TTEGTPLLDA 150
ASLDYLFAQG QYDFLRGLSP VRPTQTDEEH HEIENECLGM AVLAITHHAK 200
SNNLPLSGAG AETSYKRFIP DSLNRTIKQR NFLTRIRISN VFKNFLNEFN 250
SKTIQDSNIG LYDLKVKYLS TLETLTQGVG REIFKPKNLK VTGESEGSPA 300
QMLPLGDNGM GYEVQVYGTT GISWRRKPAP NQLILKDKPK SKKIKGDKQW 350
NDKKKDSGWT LFSDFHEITH IVIKDCCVTI YRQDNKTMEL DLFYRDAALS 400
FAALVDGYFR LTVDAHHYLC TDVAPSSVVQ NLENGCHGPI CTEYAIHKLR 450
QEGNEEGTYV LRWSCTEYNF IIMTVVCIEL DLCESRPVPQ YKNFQIETSP 500
QGYRLYGTDT FRPTLKELLE HLQGQLLRTD NLRFQLRRCC PPQPREISNL 550
LVMTTDREPV PQKKTQVSQL SFDRILKEEI VQGEHLGRGT RTNIYAGILK 600
PKSDDEDDLG GYSQEVKVVL KVLGSGHRDI SLAFFETASM MRQISHKHTA 650
LLYGVCVRHQ ENIMVEEFVQ YGPLDLFMRR QTTPLSTAWK FQVAKQLASA 700
LSYLEDKKMV HGYVCSKNIL VARDGLDGEG GPFIKLSDPG IPITVLSREE 750
CVDRIPWIAP ECVKDTANLT IAADKWSFGT TLWEICYNGE IPLKDKKLSE 800
KERFYAAQCQ LATPDCDELA KLMTHCMTYD PRQRLFFRAI VRDIVMVEKQ 850
NPSIQPVPML EVDPTVFEKR FLKKIRDLGE GHFGKVELCR YDPRGDRTGE 900
LVAVKSLKPE NREEQSNNLW REIHILRELY HENIVKYKGI CNEEGGRSIK 950
LIMEFLPAGS LKEYLPRNKA HINLKTLHNY SVQICQGMDY LGSRNYIHRD 1000
LAARNVLVEN EGTVKIGDFG LTKSIKDNEG YYTVKDDLDS PVFWYAPECL 1050
IHCKFYRASD VWSFGVTMYE LLTYCDASCS PMSVFLKLIG PTHGQMTVTR 1100
LVKVLEEGKR LPRPDDCSEQ LYNLMRRCWE ATPEKRIDFK SLIANFQQML 1150
DNL 1153
Length:1,153
Mass (Da):132,481
Last modified:July 1, 1997 - v1
Checksum:i736D5263D03E7450
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti649 – 6491T → I in CAA06673. 1 Publication
Sequence conflicti770 – 7701T → S in CAA06673. 1 Publication
Sequence conflicti799 – 7991S → T in CAA06673. 1 Publication
Sequence conflicti978 – 9781H → L in CAA06673. 1 Publication
Sequence conflicti1111 – 11111L → I in CAA06673. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U82980 mRNA. Translation: AAB54114.1.
AJ005689 mRNA. Translation: CAA06673.1.
UniGeneiDr.74470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U82980 mRNA. Translation: AAB54114.1 .
AJ005689 mRNA. Translation: CAA06673.1 .
UniGenei Dr.74470.

3D structure databases

ProteinModelPortali O12990.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ZFINi ZDB-GENE-980526-142. jak1.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000049158.
HOVERGENi HBG006195.
InParanoidi O12990.

Miscellaneous databases

PROi O12990.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020776. Tyr_kinase_non-rcpt_Jak1.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 2 hits.
[Graphical view ]
PIRSFi PIRSF000636. TyrPK_Jak. 1 hit.
PRINTSi PR01823. JANUSKINASE.
PR01824. JANUSKINASE1.
PR00109. TYRKINASE.
SMARTi SM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view ]
SUPFAMi SSF47031. SSF47031. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEi PS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Jak1 kinase is required for cell migrations and anterior specification in zebrafish embryos."
    Conway G., Margoliath A., Wong-Madden S., Roberts R.J., Gilbert W.
    Proc. Natl. Acad. Sci. U.S.A. 94:3082-3087(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-905, FUNCTION.
    Tissue: Embryo.
  2. "Gene duplication of zebrafish JAK2 homologs is accompanied by divergent embryonic expression patterns: only jak2a is expressed during erythropoiesis."
    Oates A.C., Brownlie A., Pratt S.J., Irvine D.V., Liao E.C., Paw B.H., Dorian K.J., Johnson S.L., Postlethwait J.H., Zon L.I., Wilks A.F.
    Blood 94:2622-2636(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 443-1153.
    Tissue: Embryo.

Entry informationi

Entry nameiJAK1_DANRE
AccessioniPrimary (citable) accession number: O12990
Secondary accession number(s): O73880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi