Reviewed,
UniProtKB/Swiss-Prot O12990 (JAK1_DANRE)
Last modified
October 13, 2009.
Version 83.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Tyrosine-protein kinase Jak1 EC=2.7.10.2 Alternative name(s): Janus kinase 1 Short name=Jak-1 | ||
| Gene names |
| ||
| Organism | Danio rerio (Zebrafish) (Brachydanio rerio) | ||
| Taxonomic identifier | 7955 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio |
Protein attributes
| Sequence length | 1153 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures. Ref.1 |
| Catalytic activity | ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. |
| Subcellular location | Endomembrane system; Peripheral membrane protein By similarity. Note: Wholly intracellular, possibly membrane associated By similarity. |
| Developmental stage | Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult. Ref.1 |
| Domain | Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1. |
| Sequence similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Contains 1 SH2 domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane |
| Domain | Repeat SH2 domain |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Developmental protein Kinase Transferase Tyrosine-protein kinase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylationInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoskeleton Inferred from electronic annotation. Source: InterPro extrinsic to membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Janus kinase activityInferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activityInferred from electronic annotation. Source: EC protein bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1153 | 1153 | Tyrosine-protein kinase Jak1 | PRO_0000088110 | |||||
Regions | |||||||||
| Domain | 32 – 416 | 385 | FERM | ||||||
| Domain | 435 – 540 | 106 | SH2; atypical | ||||||
| Domain | 580 – 846 | 267 | Protein kinase 1 | ||||||
| Domain | 872 – 1150 | 279 | Protein kinase 2 | ||||||
| Nucleotide binding | 878 – 886 | 9 | ATP By similarity | ||||||
| Compositional bias | 336 – 356 | 21 | Lys-rich (basic) | ||||||
Sites | |||||||||
| Active site | 1000 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 905 | 1 | ATP | ||||||
Amino acid modifications | |||||||||
| Modified residue | 1031 | 1 | Phosphotyrosine; by autocatalysis By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 905 | 1 | K → E: Loss of autophosphorylation and defects in early development. Ref.1 | ||||||
| Sequence conflict | 649 | 1 | T → I in CAA06673. Ref.2 | ||||||
| Sequence conflict | 770 | 1 | T → S in CAA06673. Ref.2 | ||||||
| Sequence conflict | 799 | 1 | S → T in CAA06673. Ref.2 | ||||||
| Sequence conflict | 978 | 1 | H → L in CAA06673. Ref.2 | ||||||
| Sequence conflict | 1111 | 1 | L → I in CAA06673. Ref.2 | ||||||
Sequences
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References
| [1] | "Jak1 kinase is required for cell migrations and anterior specification in zebrafish embryos." Conway G., Margoliath A., Wong-Madden S., Roberts R.J., Gilbert W. Proc. Natl. Acad. Sci. U.S.A. 94:3082-3087(1997) [PubMed: 9096349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-905, FUNCTION. Tissue: Embryo. |
| [2] | "Gene duplication of zebrafish JAK2 homologs is accompanied by divergent embryonic expression patterns: only jak2a is expressed during erythropoiesis." Oates A.C., Brownlie A., Pratt S.J., Irvine D.V., Liao E.C., Paw B.H., Dorian K.J., Johnson S.L., Postlethwait J.H., Zon L.I., Wilks A.F. Blood 94:2622-2636(1999) [PubMed: 10515866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 443-1153. Tissue: Embryo. |
Cross-references
Sequence databases | |
|---|---|
| U82980 mRNA. Translation: AAB54114.1. AJ005689 mRNA. Translation: CAA06673.1. | |
| IPI | IPI00482025. |
| UniGene | Dr.74470 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1FGK based on UniProtKB P11362. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | O12990. |
Genome annotation databases | |
| Ensembl | ENSDART00000038990; ENSDARP00000037399; ENSDARG00000020625; Danio rerio. [Genome view] |
Organism-specific databases | |
| ZFIN | ZDB-GENE-980526-142. jak1. |
Phylogenomic databases | |
| HOGENOM | O12990. |
| HOVERGEN | O12990. |
Gene expression databases | |
| ArrayExpress | O12990. |
| Bgee | O12990. |
Family and domain databases | |
| InterPro | IPR019749. Band_41_domain. IPR019747. FERM_CS. IPR000299. FERM_domain. IPR009127. JAK. IPR009128. JAK1. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR016251. TyrPK_Jak. [Graphical view] |
| Pfam | PF07714. Pkinase_Tyr. 2 hits. [Graphical view] |
| PIRSF | PIRSF000636. TyrPK_Jak. 1 hit. |
| PRINTS | PR01823. JANUSKINASE. PR01824. JANUSKINASE1. PR00109. TYRKINASE. |
| ProDom | PD000001. Prot_kinase. 2 hits. PD000093. SH2. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00295. B41. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 2 hits. [Graphical view] |
| PROSITE | PS00660. FERM_1. False negative. PS00661. FERM_2. False negative. PS50057. FERM_3. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 2 hits. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | JAK1_DANRE | ||||||||
| Accession | Primary (citable) accession number: O12990 Secondary accession number(s): O73880 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Zebrafish annotation project | ||||||||

Clusters with


