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O12990 (JAK1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein kinase JAK1

EC=2.7.10.2
Alternative name(s):
Janus kinase 1
Short name=JAK-1
Gene names
Name:jak1
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length1153 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures. Ref.1

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subcellular location

Endomembrane system; Peripheral membrane protein By similarity. Note: Wholly intracellular, possibly membrane associated By similarity.

Developmental stage

Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult. Ref.1

Domain

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

Contains 1 FERM domain.

Contains 2 protein kinase domains.

Contains 1 SH2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11531153Tyrosine-protein kinase JAK1
PRO_0000088110

Regions

Domain32 – 416385FERM
Domain435 – 540106SH2; atypical
Domain580 – 846267Protein kinase 1
Domain872 – 1150279Protein kinase 2
Nucleotide binding878 – 8869ATP By similarity
Compositional bias336 – 35621Lys-rich (basic)

Sites

Active site10001Proton acceptor By similarity
Binding site9051ATP

Amino acid modifications

Modified residue10311Phosphotyrosine; by autocatalysis By similarity
Modified residue10321Phosphotyrosine; by autocatalysis By similarity

Experimental info

Mutagenesis9051K → E: Loss of autophosphorylation and defects in early development. Ref.1
Sequence conflict6491T → I in CAA06673. Ref.2
Sequence conflict7701T → S in CAA06673. Ref.2
Sequence conflict7991S → T in CAA06673. Ref.2
Sequence conflict9781H → L in CAA06673. Ref.2
Sequence conflict11111L → I in CAA06673. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O12990 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 736D5263D03E7450

FASTA1,153132,481
        10         20         30         40         50         60 
MPELAVMDLG RQLCVKMKKQ RKAEMTIPTA MKGLEIHFYL ADTHQLEFFK ACYTAEDLCV 

        70         80         90        100        110        120 
EAAKRCRISP LCHNLFALYE ESQDLWYAPN HVFKVTDETS IKLHYRMRFY FTNWHGTSEI 

       130        140        150        160        170        180 
ESPVWRHTLS KQKSVLNSQK TTEGTPLLDA ASLDYLFAQG QYDFLRGLSP VRPTQTDEEH 

       190        200        210        220        230        240 
HEIENECLGM AVLAITHHAK SNNLPLSGAG AETSYKRFIP DSLNRTIKQR NFLTRIRISN 

       250        260        270        280        290        300 
VFKNFLNEFN SKTIQDSNIG LYDLKVKYLS TLETLTQGVG REIFKPKNLK VTGESEGSPA 

       310        320        330        340        350        360 
QMLPLGDNGM GYEVQVYGTT GISWRRKPAP NQLILKDKPK SKKIKGDKQW NDKKKDSGWT 

       370        380        390        400        410        420 
LFSDFHEITH IVIKDCCVTI YRQDNKTMEL DLFYRDAALS FAALVDGYFR LTVDAHHYLC 

       430        440        450        460        470        480 
TDVAPSSVVQ NLENGCHGPI CTEYAIHKLR QEGNEEGTYV LRWSCTEYNF IIMTVVCIEL 

       490        500        510        520        530        540 
DLCESRPVPQ YKNFQIETSP QGYRLYGTDT FRPTLKELLE HLQGQLLRTD NLRFQLRRCC 

       550        560        570        580        590        600 
PPQPREISNL LVMTTDREPV PQKKTQVSQL SFDRILKEEI VQGEHLGRGT RTNIYAGILK 

       610        620        630        640        650        660 
PKSDDEDDLG GYSQEVKVVL KVLGSGHRDI SLAFFETASM MRQISHKHTA LLYGVCVRHQ 

       670        680        690        700        710        720 
ENIMVEEFVQ YGPLDLFMRR QTTPLSTAWK FQVAKQLASA LSYLEDKKMV HGYVCSKNIL 

       730        740        750        760        770        780 
VARDGLDGEG GPFIKLSDPG IPITVLSREE CVDRIPWIAP ECVKDTANLT IAADKWSFGT 

       790        800        810        820        830        840 
TLWEICYNGE IPLKDKKLSE KERFYAAQCQ LATPDCDELA KLMTHCMTYD PRQRLFFRAI 

       850        860        870        880        890        900 
VRDIVMVEKQ NPSIQPVPML EVDPTVFEKR FLKKIRDLGE GHFGKVELCR YDPRGDRTGE 

       910        920        930        940        950        960 
LVAVKSLKPE NREEQSNNLW REIHILRELY HENIVKYKGI CNEEGGRSIK LIMEFLPAGS 

       970        980        990       1000       1010       1020 
LKEYLPRNKA HINLKTLHNY SVQICQGMDY LGSRNYIHRD LAARNVLVEN EGTVKIGDFG 

      1030       1040       1050       1060       1070       1080 
LTKSIKDNEG YYTVKDDLDS PVFWYAPECL IHCKFYRASD VWSFGVTMYE LLTYCDASCS 

      1090       1100       1110       1120       1130       1140 
PMSVFLKLIG PTHGQMTVTR LVKVLEEGKR LPRPDDCSEQ LYNLMRRCWE ATPEKRIDFK 

      1150 
SLIANFQQML DNL 

« Hide

References

[1]"Jak1 kinase is required for cell migrations and anterior specification in zebrafish embryos."
Conway G., Margoliath A., Wong-Madden S., Roberts R.J., Gilbert W.
Proc. Natl. Acad. Sci. U.S.A. 94:3082-3087(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-905, FUNCTION.
Tissue: Embryo.
[2]"Gene duplication of zebrafish JAK2 homologs is accompanied by divergent embryonic expression patterns: only jak2a is expressed during erythropoiesis."
Oates A.C., Brownlie A., Pratt S.J., Irvine D.V., Liao E.C., Paw B.H., Dorian K.J., Johnson S.L., Postlethwait J.H., Zon L.I., Wilks A.F.
Blood 94:2622-2636(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 443-1153.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U82980 mRNA. Translation: AAB54114.1.
AJ005689 mRNA. Translation: CAA06673.1.
UniGeneDr.74470.

3D structure databases

ProteinModelPortalO12990.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

ZFINZDB-GENE-980526-142. jak1.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000049158.
HOVERGENHBG006195.
InParanoidO12990.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020776. Tyr_kinase_non-rcpt_Jak1.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 2 hits.
[Graphical view]
PIRSFPIRSF000636. TyrPK_Jak. 1 hit.
PRINTSPR01823. JANUSKINASE.
PR01824. JANUSKINASE1.
PR00109. TYRKINASE.
SMARTSM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view]
SUPFAMSSF47031. SSF47031. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROO12990.

Entry information

Entry nameJAK1_DANRE
AccessionPrimary (citable) accession number: O12990
Secondary accession number(s): O73880
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families