Reviewed, UniProtKB/Swiss-Prot O12990 (JAK1_DANRE)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase JAK1
    EC=2.7.10.2
Alternative name(s):
    Janus kinase 1
      Short name=JAK-1

Gene names

Name:

jak1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	1153 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures. Ref.1
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Endomembrane system; Peripheral membrane protein By similarity. Note: Wholly intracellular, possibly membrane associated By similarity.
Developmental stage	Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult. Ref.1
Domain	Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat SH2 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-tyrosine phosphorylation Inferred from electronic annotation. Source: InterPro protein kinase cascade Inferred from electronic annotation. Source: InterPro
Cellular component	cytoskeleton Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Janus kinase activity Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1153	1153	Tyrosine-protein kinase JAK1		PRO_0000088110
Regions
	Domain	32 – 416	385	FERM
	Domain	435 – 540	106	SH2; atypical
	Domain	580 – 846	267	Protein kinase 1
	Domain	872 – 1150	279	Protein kinase 2
	Nucleotide binding	878 – 886	9	ATP By similarity
	Compositional bias	336 – 356	21	Lys-rich (basic)
Sites
	Active site	1000	1	Proton acceptor By similarity
	Binding site	905	1	ATP
Amino acid modifications
	Modified residue	1031	1	Phosphotyrosine; by autocatalysis By similarity
Experimental info
	Mutagenesis	905	1	K → E: Loss of autophosphorylation and defects in early development. Ref.1
	Sequence conflict	649	1	T → I in CAA06673. Ref.2
	Sequence conflict	770	1	T → S in CAA06673. Ref.2
	Sequence conflict	799	1	S → T in CAA06673. Ref.2
	Sequence conflict	978	1	H → L in CAA06673. Ref.2
	Sequence conflict	1111	1	L → I in CAA06673. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O12990-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 736D5263D03E7450	FASTA	1,153	132,481
	10 20 30 40 50 60 MPELAVMDLG RQLCVKMKKQ RKAEMTIPTA MKGLEIHFYL ADTHQLEFFK ACYTAEDLCV 70 80 90 100 110 120 EAAKRCRISP LCHNLFALYE ESQDLWYAPN HVFKVTDETS IKLHYRMRFY FTNWHGTSEI 130 140 150 160 170 180 ESPVWRHTLS KQKSVLNSQK TTEGTPLLDA ASLDYLFAQG QYDFLRGLSP VRPTQTDEEH 190 200 210 220 230 240 HEIENECLGM AVLAITHHAK SNNLPLSGAG AETSYKRFIP DSLNRTIKQR NFLTRIRISN 250 260 270 280 290 300 VFKNFLNEFN SKTIQDSNIG LYDLKVKYLS TLETLTQGVG REIFKPKNLK VTGESEGSPA 310 320 330 340 350 360 QMLPLGDNGM GYEVQVYGTT GISWRRKPAP NQLILKDKPK SKKIKGDKQW NDKKKDSGWT 370 380 390 400 410 420 LFSDFHEITH IVIKDCCVTI YRQDNKTMEL DLFYRDAALS FAALVDGYFR LTVDAHHYLC 430 440 450 460 470 480 TDVAPSSVVQ NLENGCHGPI CTEYAIHKLR QEGNEEGTYV LRWSCTEYNF IIMTVVCIEL 490 500 510 520 530 540 DLCESRPVPQ YKNFQIETSP QGYRLYGTDT FRPTLKELLE HLQGQLLRTD NLRFQLRRCC 550 560 570 580 590 600 PPQPREISNL LVMTTDREPV PQKKTQVSQL SFDRILKEEI VQGEHLGRGT RTNIYAGILK 610 620 630 640 650 660 PKSDDEDDLG GYSQEVKVVL KVLGSGHRDI SLAFFETASM MRQISHKHTA LLYGVCVRHQ 670 680 690 700 710 720 ENIMVEEFVQ YGPLDLFMRR QTTPLSTAWK FQVAKQLASA LSYLEDKKMV HGYVCSKNIL 730 740 750 760 770 780 VARDGLDGEG GPFIKLSDPG IPITVLSREE CVDRIPWIAP ECVKDTANLT IAADKWSFGT 790 800 810 820 830 840 TLWEICYNGE IPLKDKKLSE KERFYAAQCQ LATPDCDELA KLMTHCMTYD PRQRLFFRAI 850 860 870 880 890 900 VRDIVMVEKQ NPSIQPVPML EVDPTVFEKR FLKKIRDLGE GHFGKVELCR YDPRGDRTGE 910 920 930 940 950 960 LVAVKSLKPE NREEQSNNLW REIHILRELY HENIVKYKGI CNEEGGRSIK LIMEFLPAGS 970 980 990 1000 1010 1020 LKEYLPRNKA HINLKTLHNY SVQICQGMDY LGSRNYIHRD LAARNVLVEN EGTVKIGDFG 1030 1040 1050 1060 1070 1080 LTKSIKDNEG YYTVKDDLDS PVFWYAPECL IHCKFYRASD VWSFGVTMYE LLTYCDASCS 1090 1100 1110 1120 1130 1140 PMSVFLKLIG PTHGQMTVTR LVKVLEEGKR LPRPDDCSEQ LYNLMRRCWE ATPEKRIDFK 1150 SLIANFQQML DNL

References

[1]

"Jak1 kinase is required for cell migrations and anterior specification in zebrafish embryos." Conway G., Margoliath A., Wong-Madden S., Roberts R.J., Gilbert W. Proc. Natl. Acad. Sci. U.S.A. 94:3082-3087(1997) [PubMed: 9096349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-905, FUNCTION. Tissue: Embryo.

[2]

"Gene duplication of zebrafish JAK2 homologs is accompanied by divergent embryonic expression patterns: only jak2a is expressed during erythropoiesis." Oates A.C., Brownlie A., Pratt S.J., Irvine D.V., Liao E.C., Paw B.H., Dorian K.J., Johnson S.L., Postlethwait J.H., Zon L.I., Wilks A.F. Blood 94:2622-2636(1999) [PubMed: 10515866] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 443-1153. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82980 mRNA. Translation: AAB54114.1. AJ005689 mRNA. Translation: CAA06673.1.
IPI	IPI00482025.
UniGene	Dr.74470
3D structure databases
SMR	O12990. Positions 433-842, 633-875, 864-1151.
ModBase	Search...
Protein-protein interaction databases
STRING	O12990.
Genome annotation databases
Ensembl	ENSDART00000038990; ENSDARP00000037399; ENSDARG00000020625; Danio rerio. [Genome view]
Organism-specific databases
ZFIN	ZDB-GENE-980526-142. jak1.
Phylogenomic databases
eggNOG	fiNOG10982.
HOGENOM	HBG445257.
HOVERGEN	O12990.
InParanoid	O12990.
Gene expression databases
ArrayExpress	O12990.
Bgee	O12990.
Family and domain databases
InterPro	IPR019749. Band_41_domain. IPR000299. FERM_domain. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2. IPR020776. Tyr_kinase_non-rcpt_Jak1. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR009128. Tyr_prot_kinase_JAK1. IPR009127. Tyr_prot_kinase_JAK_cons-reg. [Graphical view]
PANTHER	PTHR23256:SF283. Tyr_kinase_non-rcpt_Jak1. 1 hit. PTHR23256. Tyr_prot_kinase. 1 hit.
PIRSF	PIRSF000636. TyrPK_Jak. 1 hit.
PRINTS	PR01823. JANUSKINASE. PR01824. JANUSKINASE1.
SMART	SM00295. B41. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 2 hits. [Graphical view]
PROSITE	PS00660. FERM_1. False negative. PS00661. FERM_2. False negative. PS50057. FERM_3. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 2 hits. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JAK1_DANRE

Accession

Primary (citable) accession number: O12990
Secondary accession number(s): O73880

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	July 1, 1997
Last modified:	February 9, 2010
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project