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Protein

Tyrosine-protein kinase JAK1

Gene

jak1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei905 – 9051ATP
Active sitei1000 – 10001Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi878 – 8869ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 928.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase JAK1 (EC:2.7.10.2)
Alternative name(s):
Janus kinase 1
Short name:
JAK-1
Gene namesi
Name:jak1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-980526-142. jak1.

Subcellular locationi

Endomembrane system By similarity; Peripheral membrane protein By similarity
Note: Wholly intracellular, possibly membrane associated.By similarity

GO - Cellular componenti

  1. cytoskeleton Source: InterPro
  2. endomembrane system Source: UniProtKB-SubCell
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi905 – 9051K → E: Loss of autophosphorylation and defects in early development. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11531153Tyrosine-protein kinase JAK1PRO_0000088110Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1031 – 10311Phosphotyrosine; by autocatalysisBy similarity
Modified residuei1032 – 10321Phosphotyrosine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Developmental stagei

Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO12990.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 416385FERMPROSITE-ProRule annotationAdd
BLAST
Domaini435 – 540106SH2; atypicalAdd
BLAST
Domaini580 – 846267Protein kinase 1PROSITE-ProRule annotationAdd
BLAST
Domaini872 – 1150279Protein kinase 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi336 – 35621Lys-rich (basic)Add
BLAST

Domaini

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.PROSITE-ProRule annotation
Contains 1 FERM domain.PROSITE-ProRule annotation
Contains 2 protein kinase domains.PROSITE-ProRule annotation
Contains 1 SH2 domain.Curated

Keywords - Domaini

Repeat, SH2 domain

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000049158.
HOVERGENiHBG006195.
InParanoidiO12990.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020776. Tyr_kinase_non-rcpt_Jak1.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 2 hits.
[Graphical view]
PIRSFiPIRSF000636. TyrPK_Jak. 1 hit.
PRINTSiPR01823. JANUSKINASE.
PR01824. JANUSKINASE1.
PR00109. TYRKINASE.
SMARTiSM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O12990-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPELAVMDLG RQLCVKMKKQ RKAEMTIPTA MKGLEIHFYL ADTHQLEFFK
60 70 80 90 100
ACYTAEDLCV EAAKRCRISP LCHNLFALYE ESQDLWYAPN HVFKVTDETS
110 120 130 140 150
IKLHYRMRFY FTNWHGTSEI ESPVWRHTLS KQKSVLNSQK TTEGTPLLDA
160 170 180 190 200
ASLDYLFAQG QYDFLRGLSP VRPTQTDEEH HEIENECLGM AVLAITHHAK
210 220 230 240 250
SNNLPLSGAG AETSYKRFIP DSLNRTIKQR NFLTRIRISN VFKNFLNEFN
260 270 280 290 300
SKTIQDSNIG LYDLKVKYLS TLETLTQGVG REIFKPKNLK VTGESEGSPA
310 320 330 340 350
QMLPLGDNGM GYEVQVYGTT GISWRRKPAP NQLILKDKPK SKKIKGDKQW
360 370 380 390 400
NDKKKDSGWT LFSDFHEITH IVIKDCCVTI YRQDNKTMEL DLFYRDAALS
410 420 430 440 450
FAALVDGYFR LTVDAHHYLC TDVAPSSVVQ NLENGCHGPI CTEYAIHKLR
460 470 480 490 500
QEGNEEGTYV LRWSCTEYNF IIMTVVCIEL DLCESRPVPQ YKNFQIETSP
510 520 530 540 550
QGYRLYGTDT FRPTLKELLE HLQGQLLRTD NLRFQLRRCC PPQPREISNL
560 570 580 590 600
LVMTTDREPV PQKKTQVSQL SFDRILKEEI VQGEHLGRGT RTNIYAGILK
610 620 630 640 650
PKSDDEDDLG GYSQEVKVVL KVLGSGHRDI SLAFFETASM MRQISHKHTA
660 670 680 690 700
LLYGVCVRHQ ENIMVEEFVQ YGPLDLFMRR QTTPLSTAWK FQVAKQLASA
710 720 730 740 750
LSYLEDKKMV HGYVCSKNIL VARDGLDGEG GPFIKLSDPG IPITVLSREE
760 770 780 790 800
CVDRIPWIAP ECVKDTANLT IAADKWSFGT TLWEICYNGE IPLKDKKLSE
810 820 830 840 850
KERFYAAQCQ LATPDCDELA KLMTHCMTYD PRQRLFFRAI VRDIVMVEKQ
860 870 880 890 900
NPSIQPVPML EVDPTVFEKR FLKKIRDLGE GHFGKVELCR YDPRGDRTGE
910 920 930 940 950
LVAVKSLKPE NREEQSNNLW REIHILRELY HENIVKYKGI CNEEGGRSIK
960 970 980 990 1000
LIMEFLPAGS LKEYLPRNKA HINLKTLHNY SVQICQGMDY LGSRNYIHRD
1010 1020 1030 1040 1050
LAARNVLVEN EGTVKIGDFG LTKSIKDNEG YYTVKDDLDS PVFWYAPECL
1060 1070 1080 1090 1100
IHCKFYRASD VWSFGVTMYE LLTYCDASCS PMSVFLKLIG PTHGQMTVTR
1110 1120 1130 1140 1150
LVKVLEEGKR LPRPDDCSEQ LYNLMRRCWE ATPEKRIDFK SLIANFQQML

DNL
Length:1,153
Mass (Da):132,481
Last modified:July 1, 1997 - v1
Checksum:i736D5263D03E7450
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti649 – 6491T → I in CAA06673 (PubMed:10515866).Curated
Sequence conflicti770 – 7701T → S in CAA06673 (PubMed:10515866).Curated
Sequence conflicti799 – 7991S → T in CAA06673 (PubMed:10515866).Curated
Sequence conflicti978 – 9781H → L in CAA06673 (PubMed:10515866).Curated
Sequence conflicti1111 – 11111L → I in CAA06673 (PubMed:10515866).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82980 mRNA. Translation: AAB54114.1.
AJ005689 mRNA. Translation: CAA06673.1.
UniGeneiDr.74470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82980 mRNA. Translation: AAB54114.1.
AJ005689 mRNA. Translation: CAA06673.1.
UniGeneiDr.74470.

3D structure databases

ProteinModelPortaliO12990.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ZFINiZDB-GENE-980526-142. jak1.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000049158.
HOVERGENiHBG006195.
InParanoidiO12990.

Enzyme and pathway databases

BRENDAi2.7.10.2. 928.

Miscellaneous databases

PROiO12990.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020776. Tyr_kinase_non-rcpt_Jak1.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 2 hits.
[Graphical view]
PIRSFiPIRSF000636. TyrPK_Jak. 1 hit.
PRINTSiPR01823. JANUSKINASE.
PR01824. JANUSKINASE1.
PR00109. TYRKINASE.
SMARTiSM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Jak1 kinase is required for cell migrations and anterior specification in zebrafish embryos."
    Conway G., Margoliath A., Wong-Madden S., Roberts R.J., Gilbert W.
    Proc. Natl. Acad. Sci. U.S.A. 94:3082-3087(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-905, FUNCTION.
    Tissue: Embryo.
  2. "Gene duplication of zebrafish JAK2 homologs is accompanied by divergent embryonic expression patterns: only jak2a is expressed during erythropoiesis."
    Oates A.C., Brownlie A., Pratt S.J., Irvine D.V., Liao E.C., Paw B.H., Dorian K.J., Johnson S.L., Postlethwait J.H., Zon L.I., Wilks A.F.
    Blood 94:2622-2636(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 443-1153.
    Tissue: Embryo.

Entry informationi

Entry nameiJAK1_DANRE
AccessioniPrimary (citable) accession number: O12990
Secondary accession number(s): O73880
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: July 1, 1997
Last modified: April 1, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.