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Protein

Tyrosine-protein kinase JAK1

Gene

jak1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Cofactori

Mg2+CuratedNote: Mn2+ was used in the in vitro kinase assay but Mg2+ is likely to be the in vivo cofactor.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei905ATP1
Active sitei1000Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi878 – 886ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 928

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase JAK1 (EC:2.7.10.2)
Alternative name(s):
Janus kinase 1
Short name:
JAK-1
Gene namesi
Name:jak1
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Unplaced

Organism-specific databases

ZFINiZDB-GENE-980526-142 jak1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi905K → E: Loss of autophosphorylation and defects in early development. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000881101 – 1153Tyrosine-protein kinase JAK1Add BLAST1153

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1031Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1032Phosphotyrosine; by autocatalysisBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO12990

Expressioni

Developmental stagei

Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult.1 Publication

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000037399

Structurei

3D structure databases

ProteinModelPortaliO12990
SMRiO12990
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 416FERMPROSITE-ProRule annotationAdd BLAST385
Domaini435 – 540SH2; atypicalAdd BLAST106
Domaini580 – 846Protein kinase 1PROSITE-ProRule annotationAdd BLAST267
Domaini872 – 1150Protein kinase 2PROSITE-ProRule annotationAdd BLAST279

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi336 – 356Lys-rich (basic)Add BLAST21

Domaini

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain

Phylogenomic databases

eggNOGiKOG0197 Eukaryota
COG0515 LUCA
HOGENOMiHOG000049158
HOVERGENiHBG006195
InParanoidiO12990

Family and domain databases

Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR019749 Band_41_domain
IPR035963 FERM_2
IPR000299 FERM_domain
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR016251 Tyr_kinase_non-rcpt_Jak/Tyk2
IPR020776 Tyr_kinase_non-rcpt_Jak1
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 2 hits
PIRSFiPIRSF000636 TyrPK_Jak, 1 hit
PRINTSiPR01823 JANUSKINASE
PR01824 JANUSKINASE1
PR00109 TYRKINASE
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM00252 SH2, 1 hit
SM00219 TyrKc, 2 hits
SUPFAMiSSF47031 SSF47031, 1 hit
SSF55550 SSF55550, 1 hit
SSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS50057 FERM_3, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 2 hits
PS00109 PROTEIN_KINASE_TYR, 1 hit

Sequencei

Sequence statusi: Complete.

O12990-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPELAVMDLG RQLCVKMKKQ RKAEMTIPTA MKGLEIHFYL ADTHQLEFFK
60 70 80 90 100
ACYTAEDLCV EAAKRCRISP LCHNLFALYE ESQDLWYAPN HVFKVTDETS
110 120 130 140 150
IKLHYRMRFY FTNWHGTSEI ESPVWRHTLS KQKSVLNSQK TTEGTPLLDA
160 170 180 190 200
ASLDYLFAQG QYDFLRGLSP VRPTQTDEEH HEIENECLGM AVLAITHHAK
210 220 230 240 250
SNNLPLSGAG AETSYKRFIP DSLNRTIKQR NFLTRIRISN VFKNFLNEFN
260 270 280 290 300
SKTIQDSNIG LYDLKVKYLS TLETLTQGVG REIFKPKNLK VTGESEGSPA
310 320 330 340 350
QMLPLGDNGM GYEVQVYGTT GISWRRKPAP NQLILKDKPK SKKIKGDKQW
360 370 380 390 400
NDKKKDSGWT LFSDFHEITH IVIKDCCVTI YRQDNKTMEL DLFYRDAALS
410 420 430 440 450
FAALVDGYFR LTVDAHHYLC TDVAPSSVVQ NLENGCHGPI CTEYAIHKLR
460 470 480 490 500
QEGNEEGTYV LRWSCTEYNF IIMTVVCIEL DLCESRPVPQ YKNFQIETSP
510 520 530 540 550
QGYRLYGTDT FRPTLKELLE HLQGQLLRTD NLRFQLRRCC PPQPREISNL
560 570 580 590 600
LVMTTDREPV PQKKTQVSQL SFDRILKEEI VQGEHLGRGT RTNIYAGILK
610 620 630 640 650
PKSDDEDDLG GYSQEVKVVL KVLGSGHRDI SLAFFETASM MRQISHKHTA
660 670 680 690 700
LLYGVCVRHQ ENIMVEEFVQ YGPLDLFMRR QTTPLSTAWK FQVAKQLASA
710 720 730 740 750
LSYLEDKKMV HGYVCSKNIL VARDGLDGEG GPFIKLSDPG IPITVLSREE
760 770 780 790 800
CVDRIPWIAP ECVKDTANLT IAADKWSFGT TLWEICYNGE IPLKDKKLSE
810 820 830 840 850
KERFYAAQCQ LATPDCDELA KLMTHCMTYD PRQRLFFRAI VRDIVMVEKQ
860 870 880 890 900
NPSIQPVPML EVDPTVFEKR FLKKIRDLGE GHFGKVELCR YDPRGDRTGE
910 920 930 940 950
LVAVKSLKPE NREEQSNNLW REIHILRELY HENIVKYKGI CNEEGGRSIK
960 970 980 990 1000
LIMEFLPAGS LKEYLPRNKA HINLKTLHNY SVQICQGMDY LGSRNYIHRD
1010 1020 1030 1040 1050
LAARNVLVEN EGTVKIGDFG LTKSIKDNEG YYTVKDDLDS PVFWYAPECL
1060 1070 1080 1090 1100
IHCKFYRASD VWSFGVTMYE LLTYCDASCS PMSVFLKLIG PTHGQMTVTR
1110 1120 1130 1140 1150
LVKVLEEGKR LPRPDDCSEQ LYNLMRRCWE ATPEKRIDFK SLIANFQQML

DNL
Length:1,153
Mass (Da):132,481
Last modified:July 1, 1997 - v1
Checksum:i736D5263D03E7450
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti649T → I in CAA06673 (PubMed:10515866).Curated1
Sequence conflicti770T → S in CAA06673 (PubMed:10515866).Curated1
Sequence conflicti799S → T in CAA06673 (PubMed:10515866).Curated1
Sequence conflicti978H → L in CAA06673 (PubMed:10515866).Curated1
Sequence conflicti1111L → I in CAA06673 (PubMed:10515866).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82980 mRNA Translation: AAB54114.1
AJ005689 mRNA Translation: CAA06673.1
UniGeneiDr.74470

Similar proteinsi

Entry informationi

Entry nameiJAK1_DANRE
AccessioniPrimary (citable) accession number: O12990
Secondary accession number(s): O73880
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: July 1, 1997
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health