Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O12990

- JAK1_DANRE

UniProt

O12990 - JAK1_DANRE

Protein

Tyrosine-protein kinase JAK1

Gene

jak1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Jul 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Tyrosine kinase of the non-receptor type. Appears to be required in early development for specific cell migrations (epiboly), expression of homeobox protein goosecoid and formation of anterior structures.1 Publication

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei905 – 9051ATP
    Active sitei1000 – 10001Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi878 – 8869ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. multicellular organismal development Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase JAK1 (EC:2.7.10.2)
    Alternative name(s):
    Janus kinase 1
    Short name:
    JAK-1
    Gene namesi
    Name:jak1
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Organism-specific databases

    ZFINiZDB-GENE-980526-142. jak1.

    Subcellular locationi

    Endomembrane system By similarity; Peripheral membrane protein By similarity
    Note: Wholly intracellular, possibly membrane associated.By similarity

    GO - Cellular componenti

    1. cytoskeleton Source: InterPro
    2. endomembrane system Source: UniProtKB-SubCell
    3. membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi905 – 9051K → E: Loss of autophosphorylation and defects in early development. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11531153Tyrosine-protein kinase JAK1PRO_0000088110Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1031 – 10311Phosphotyrosine; by autocatalysisBy similarity
    Modified residuei1032 – 10321Phosphotyrosine; by autocatalysisBy similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Developmental stagei

    Present in the unfertilized egg through to the blastula stage where it is distributed uniformly. Levels drop rapidly at four hours development, remain very low until 10 hours, then gradually increase from 12 hours with a rapid increase at 48 hours. At 48 hours it is concentrated in the region of the gill arches. Also present in the adult.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliO12990.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 416385FERMPROSITE-ProRule annotationAdd
    BLAST
    Domaini435 – 540106SH2; atypicalAdd
    BLAST
    Domaini580 – 846267Protein kinase 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini872 – 1150279Protein kinase 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi336 – 35621Lys-rich (basic)Add
    BLAST

    Domaini

    Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.PROSITE-ProRule annotation
    Contains 1 FERM domain.PROSITE-ProRule annotation
    Contains 2 protein kinase domains.PROSITE-ProRule annotation
    Contains 1 SH2 domain.Curated

    Keywords - Domaini

    Repeat, SH2 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000049158.
    HOVERGENiHBG006195.
    InParanoidiO12990.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR019749. Band_41_domain.
    IPR019748. FERM_central.
    IPR000299. FERM_domain.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
    IPR020776. Tyr_kinase_non-rcpt_Jak1.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000636. TyrPK_Jak. 1 hit.
    PRINTSiPR01823. JANUSKINASE.
    PR01824. JANUSKINASE1.
    PR00109. TYRKINASE.
    SMARTiSM00295. B41. 1 hit.
    SM00252. SH2. 1 hit.
    SM00219. TyrKc. 2 hits.
    [Graphical view]
    SUPFAMiSSF47031. SSF47031. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 2 hits.
    PROSITEiPS50057. FERM_3. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 2 hits.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O12990-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPELAVMDLG RQLCVKMKKQ RKAEMTIPTA MKGLEIHFYL ADTHQLEFFK     50
    ACYTAEDLCV EAAKRCRISP LCHNLFALYE ESQDLWYAPN HVFKVTDETS 100
    IKLHYRMRFY FTNWHGTSEI ESPVWRHTLS KQKSVLNSQK TTEGTPLLDA 150
    ASLDYLFAQG QYDFLRGLSP VRPTQTDEEH HEIENECLGM AVLAITHHAK 200
    SNNLPLSGAG AETSYKRFIP DSLNRTIKQR NFLTRIRISN VFKNFLNEFN 250
    SKTIQDSNIG LYDLKVKYLS TLETLTQGVG REIFKPKNLK VTGESEGSPA 300
    QMLPLGDNGM GYEVQVYGTT GISWRRKPAP NQLILKDKPK SKKIKGDKQW 350
    NDKKKDSGWT LFSDFHEITH IVIKDCCVTI YRQDNKTMEL DLFYRDAALS 400
    FAALVDGYFR LTVDAHHYLC TDVAPSSVVQ NLENGCHGPI CTEYAIHKLR 450
    QEGNEEGTYV LRWSCTEYNF IIMTVVCIEL DLCESRPVPQ YKNFQIETSP 500
    QGYRLYGTDT FRPTLKELLE HLQGQLLRTD NLRFQLRRCC PPQPREISNL 550
    LVMTTDREPV PQKKTQVSQL SFDRILKEEI VQGEHLGRGT RTNIYAGILK 600
    PKSDDEDDLG GYSQEVKVVL KVLGSGHRDI SLAFFETASM MRQISHKHTA 650
    LLYGVCVRHQ ENIMVEEFVQ YGPLDLFMRR QTTPLSTAWK FQVAKQLASA 700
    LSYLEDKKMV HGYVCSKNIL VARDGLDGEG GPFIKLSDPG IPITVLSREE 750
    CVDRIPWIAP ECVKDTANLT IAADKWSFGT TLWEICYNGE IPLKDKKLSE 800
    KERFYAAQCQ LATPDCDELA KLMTHCMTYD PRQRLFFRAI VRDIVMVEKQ 850
    NPSIQPVPML EVDPTVFEKR FLKKIRDLGE GHFGKVELCR YDPRGDRTGE 900
    LVAVKSLKPE NREEQSNNLW REIHILRELY HENIVKYKGI CNEEGGRSIK 950
    LIMEFLPAGS LKEYLPRNKA HINLKTLHNY SVQICQGMDY LGSRNYIHRD 1000
    LAARNVLVEN EGTVKIGDFG LTKSIKDNEG YYTVKDDLDS PVFWYAPECL 1050
    IHCKFYRASD VWSFGVTMYE LLTYCDASCS PMSVFLKLIG PTHGQMTVTR 1100
    LVKVLEEGKR LPRPDDCSEQ LYNLMRRCWE ATPEKRIDFK SLIANFQQML 1150
    DNL 1153
    Length:1,153
    Mass (Da):132,481
    Last modified:July 1, 1997 - v1
    Checksum:i736D5263D03E7450
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti649 – 6491T → I in CAA06673. (PubMed:10515866)Curated
    Sequence conflicti770 – 7701T → S in CAA06673. (PubMed:10515866)Curated
    Sequence conflicti799 – 7991S → T in CAA06673. (PubMed:10515866)Curated
    Sequence conflicti978 – 9781H → L in CAA06673. (PubMed:10515866)Curated
    Sequence conflicti1111 – 11111L → I in CAA06673. (PubMed:10515866)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U82980 mRNA. Translation: AAB54114.1.
    AJ005689 mRNA. Translation: CAA06673.1.
    UniGeneiDr.74470.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U82980 mRNA. Translation: AAB54114.1 .
    AJ005689 mRNA. Translation: CAA06673.1 .
    UniGenei Dr.74470.

    3D structure databases

    ProteinModelPortali O12990.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    ZFINi ZDB-GENE-980526-142. jak1.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000049158.
    HOVERGENi HBG006195.
    InParanoidi O12990.

    Miscellaneous databases

    PROi O12990.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR019749. Band_41_domain.
    IPR019748. FERM_central.
    IPR000299. FERM_domain.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
    IPR020776. Tyr_kinase_non-rcpt_Jak1.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000636. TyrPK_Jak. 1 hit.
    PRINTSi PR01823. JANUSKINASE.
    PR01824. JANUSKINASE1.
    PR00109. TYRKINASE.
    SMARTi SM00295. B41. 1 hit.
    SM00252. SH2. 1 hit.
    SM00219. TyrKc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47031. SSF47031. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 2 hits.
    PROSITEi PS50057. FERM_3. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 2 hits.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Jak1 kinase is required for cell migrations and anterior specification in zebrafish embryos."
      Conway G., Margoliath A., Wong-Madden S., Roberts R.J., Gilbert W.
      Proc. Natl. Acad. Sci. U.S.A. 94:3082-3087(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, MUTAGENESIS OF LYS-905, FUNCTION.
      Tissue: Embryo.
    2. "Gene duplication of zebrafish JAK2 homologs is accompanied by divergent embryonic expression patterns: only jak2a is expressed during erythropoiesis."
      Oates A.C., Brownlie A., Pratt S.J., Irvine D.V., Liao E.C., Paw B.H., Dorian K.J., Johnson S.L., Postlethwait J.H., Zon L.I., Wilks A.F.
      Blood 94:2622-2636(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 443-1153.
      Tissue: Embryo.

    Entry informationi

    Entry nameiJAK1_DANRE
    AccessioniPrimary (citable) accession number: O12990
    Secondary accession number(s): O73880
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 27, 2002
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3