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Protein

Beta-1,3-N-acetylglucosaminyltransferase radical fringe

Gene

RFNG

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Plays an important role in limb outgrowth, it directs the formation and positioning of the apical ectodermal ridge (AER), one of the key organizer centers of vertebrate limb development.

Catalytic activityi

Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201SubstrateBy similarity
Binding sitei193 – 1931SubstrateBy similarity
Metal bindingi194 – 1941ManganeseBy similarity
Active sitei283 – 2831By similarity
Metal bindingi307 – 3071ManganeseBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Keywords - Biological processi

Differentiation, Neurogenesis

Keywords - Ligandi

Manganese, Metal-binding

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-N-acetylglucosaminyltransferase radical fringe (EC:2.4.1.222)
Alternative name(s):
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene namesi
Name:RFNG
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010CytoplasmicSequence analysis
Transmembranei11 – 2717Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 372345LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Beta-1,3-N-acetylglucosaminyltransferase radical fringePRO_0000219188Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi159 – 1591N-linked (GlcNAc...)Sequence analysis
Disulfide bondi160 ↔ 171By similarity
Disulfide bondi189 ↔ 253By similarity
Disulfide bondi357 ↔ 366By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO12972.
PRIDEiO12972.

Expressioni

Developmental stagei

Detected at stage 15 in the presumptive dorsal-limb ectoderm. At stages 16-18 expression is restricted to the dorsal side with higher concentration at the dorsoventral boundary and later in the AER. Expression remains restricted to the dorsal ectoderm and the AER until stages 22-24 when it starts to fade in the dorsal ectoderm, but remains in the AER until the last stage examined (27).

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000004473.

Structurei

3D structure databases

ProteinModelPortaliO12972.
SMRiO12972. Positions 101-368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiO12972.
KOiK05948.
PhylomeDBiO12972.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.

Sequencei

Sequence statusi: Complete.

O12972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSSCLGLRR TCFLLSVTAA AVLLLLLPRG QPPAAPRRRP PPAGPSRPSP
60 70 80 90 100
KREARPAGSD VPGDRGGGSG AAGGGRGVAG SPWPSRRVRM GPPGGSAKES
110 120 130 140 150
LELKDIFIAV KTTRKYHKTR LELLFQTWIS RARGQTFIFT DWEDRELRLK
160 170 180 190 200
AGDHMINTNC SAVHTRQALC CKMSVEYDKF LESGQKWFCH VDDDNYVNPR
210 220 230 240 250
TLLRLLSAFS PSQDVYVGRP SLDHPIEAAD HVQSDGSKTS VKFWFATGGA
260 270 280 290 300
GFCISRGLAL KMSPWASLGN FISTAERVRL PDDCTIGYII EGLLEVKLLH
310 320 330 340 350
SPLFHSHLEN LQRLQGESVL QQVTLSYGDP ENKHNVVSVG GVFGLQQDPT
360 370
RFKSVHCLLY PDTIWCPNKK MS
Length:372
Mass (Da):40,962
Last modified:January 10, 2003 - v2
Checksum:i84CB7B74A4B81C6D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21N → S in AAC60100 (PubMed:9121552).Curated
Sequence conflicti11 – 111T → A in AAC60100 (PubMed:9121552).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82088 mRNA. Translation: AAC60107.1.
U91850 mRNA. Translation: AAC60100.1.
RefSeqiNP_990278.1. NM_204947.1.
UniGeneiGga.4783.

Genome annotation databases

GeneIDi395789.
KEGGigga:395789.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82088 mRNA. Translation: AAC60107.1.
U91850 mRNA. Translation: AAC60100.1.
RefSeqiNP_990278.1. NM_204947.1.
UniGeneiGga.4783.

3D structure databases

ProteinModelPortaliO12972.
SMRiO12972. Positions 101-368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000004473.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Proteomic databases

PaxDbiO12972.
PRIDEiO12972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi395789.
KEGGigga:395789.

Organism-specific databases

CTDi5986.

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiO12972.
KOiK05948.
PhylomeDBiO12972.

Miscellaneous databases

PROiO12972.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Radical fringe positions the apical ectodermal ridge at the dorsoventral boundary of the vertebrate limb."
    Rodriguez-Esteban C., Schwabe J.W.R., De La Pena J., Foys B., Eshelman B., Izpisua-Belmonte J.-C.
    Nature 386:360-366(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Limb.
  2. "Expression of Radical fringe in limb-bud ectoderm regulates apical ectodermal ridge formation."
    Laufer E., Dahn R., Orozco O.E., Yeo C.-Y., Pisenti J., Henrique D., Abbott U.K., Fallon J.F., Tabin C.
    Nature 386:366-373(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiRFNG_CHICK
AccessioniPrimary (citable) accession number: O12972
Secondary accession number(s): O12970
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: January 10, 2003
Last modified: June 8, 2016
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.