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Protein

Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe

Gene

LFNG

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Essential mediator of somite segmentation and patterning (By similarity).By similarity

Catalytic activityi

Transfers a beta-D-GlcNAc residue from UDP-D-GlcNAc to the fucose residue of a fucosylated protein acceptor.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei113SubstrateBy similarity1
Binding sitei185SubstrateBy similarity1
Metal bindingi186ManganeseBy similarity1
Active sitei274By similarity1
Metal bindingi298ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe (EC:2.4.1.222)
Alternative name(s):
O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Gene namesi
Name:LFNG
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini30 – 363LumenalSequence analysisAdd BLAST334

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002191791 – 363Beta-1,3-N-acetylglucosaminyltransferase lunatic fringeAdd BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi151N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi152 ↔ 163By similarity
Disulfide bondi181 ↔ 244By similarity
Disulfide bondi348 ↔ 357By similarity

Post-translational modificationi

A soluble form may be derived from the membrane form by proteolytic processing.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei77 – 78Cleavage; by furin-like proteaseSequence analysis2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO12971.

Expressioni

Developmental stagei

At stage 13 it is detected in the presomitic mesoderm, transiently observed before segmentation, and in the rostral part of the neural tube. Up-regulated in the neural tube, the retina and the otic vesicle from this stage on. At stage 17 a distinct stripe pattern was clear in the hindbrain and the spinal chord. Also found in the neuroepithelium of the midbrain and forebrain. The expression was down-regulated with the termination of neurogenesis at stage 36.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000006798.

Structurei

3D structure databases

ProteinModelPortaliO12971.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 31 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
GeneTreeiENSGT00390000009913.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiO12971.
KOiK05948.
PhylomeDBiO12971.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.

Sequencei

Sequence statusi: Complete.

O12971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKSCGRKLL LSLVGSMFTC LLVLMVEPPG RPGLARGEAG GAQRALQSLG
60 70 80 90 100
AARAAGQGAP GLRSFADYFG RLSRARRELP AAPPSPPRPP AEDITPRDVF
110 120 130 140 150
IAVKTTKKFH KARLELLLDT WISRNRDMTF IFTDGEDEEL KKQARNVINT
160 170 180 190 200
NCSAAHSRQA LSCKMAVEYD KFIESGRKWF CHVDDDNYVN VRTLVKLLSS
210 220 230 240 250
YPHTQDIYIG KPSLDRPIQA TERISENKMH PVHFWFATGG AGFCISRGLA
260 270 280 290 300
LKMSPWASGG HFMSTAEKIR LPDDCTIGYI IESVLGVKLI RSNLFHSHLE
310 320 330 340 350
NLHQVPKTEI HKQVTLSYGM FENKRNSIHM KGAFSVEEDP SRFRSVHCLL
360
YPDTPWCPSN VVY
Length:363
Mass (Da):40,658
Last modified:July 1, 1997 - v1
Checksum:i433579BDA394F999
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161L → C in AAB60860 (PubMed:9175788).Curated1
Sequence conflicti267E → Q in AAB60860 (PubMed:9175788).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91849 mRNA. Translation: AAC60099.1.
U97157 mRNA. Translation: AAB60860.1.
PIRiJC5536.
RefSeqiNP_990279.1. NM_204948.1.
UniGeneiGga.3180.

Genome annotation databases

EnsembliENSGALT00000073543; ENSGALP00000045107; ENSGALG00000038515.
GeneIDi395790.
KEGGigga:395790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91849 mRNA. Translation: AAC60099.1.
U97157 mRNA. Translation: AAB60860.1.
PIRiJC5536.
RefSeqiNP_990279.1. NM_204948.1.
UniGeneiGga.3180.

3D structure databases

ProteinModelPortaliO12971.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000006798.

Protein family/group databases

CAZyiGT31. Glycosyltransferase Family 31.

Proteomic databases

PaxDbiO12971.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000073543; ENSGALP00000045107; ENSGALG00000038515.
GeneIDi395790.
KEGGigga:395790.

Organism-specific databases

CTDi3955.

Phylogenomic databases

eggNOGiENOG410II9W. Eukaryota.
ENOG410XS8Y. LUCA.
GeneTreeiENSGT00390000009913.
HOGENOMiHOG000046678.
HOVERGENiHBG007986.
InParanoidiO12971.
KOiK05948.
PhylomeDBiO12971.

Miscellaneous databases

PROiO12971.

Family and domain databases

InterProiIPR017374. Fringe.
IPR003378. Fringe-like.
[Graphical view]
PfamiPF02434. Fringe. 1 hit.
[Graphical view]
PIRSFiPIRSF038073. B-acetylgalactosaminyltfrase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLFNG_CHICK
AccessioniPrimary (citable) accession number: O12971
Secondary accession number(s): O13130
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2003
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.