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Protein

Protein-S-isoprenylcysteine O-methyltransferase

Gene

icmt

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.

Catalytic activityi

S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.PROSITE-ProRule annotation

Cofactori

Zn2+By similarityNote: Divalent metal cations. Probably Zn2+.By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100)
Alternative name(s):
Farnesyl cysteine carboxyl methyltransferase
Short name:
FCMT
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name:
PPMT
Prenylcysteine carboxyl methyltransferase
Short name:
pcCMT
Gene namesi
Name:icmt
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6068654. icmt.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 29HelicalSequence analysisAdd BLAST17
Topological domaini30 – 45LumenalSequence analysisAdd BLAST16
Transmembranei46 – 63HelicalSequence analysisAdd BLAST18
Topological domaini64 – 73CytoplasmicSequence analysis10
Transmembranei74 – 91HelicalSequence analysisAdd BLAST18
Topological domaini92 – 96LumenalSequence analysis5
Transmembranei97 – 116HelicalSequence analysisAdd BLAST20
Topological domaini117 – 135CytoplasmicSequence analysisAdd BLAST19
Transmembranei136 – 153HelicalSequence analysisAdd BLAST18
Topological domaini154 – 158LumenalSequence analysis5
Transmembranei159 – 178HelicalSequence analysisAdd BLAST20
Topological domaini179 – 216CytoplasmicSequence analysisAdd BLAST38
Transmembranei217 – 232HelicalSequence analysisAdd BLAST16
Topological domaini233LumenalSequence analysis1
Transmembranei234 – 248HelicalSequence analysisAdd BLAST15
Topological domaini249 – 288CytoplasmicSequence analysisAdd BLAST40

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002098971 – 288Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST288

Structurei

3D structure databases

ProteinModelPortaliO12947.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG019034.
KOiK00587.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O12947-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGARLLQEG RVSIVSFTLG ASVISLPLLT SSFTEQTLLA AAPGRIALVF
60 70 80 90 100
FIAALNGLLL LLYKAQLYQV AIRASFLGFA FGCGLLLSIT QSPWKPFGWY
110 120 130 140 150
VCSLSFFHYS EYLVTAMNNP RSLSIDSFLL NHSLEYTLAA LSSWVEFTIE
160 170 180 190 200
TTIYPDLKQI TWLSVIGLIM VLFGEVLRKC AMLTAGSNFN HIVQNEKSDS
210 220 230 240 250
HTLVTSGVYS WFRHPSYVGW FYWSIGTQVL LCNPLCLVGY TLASWRFFSE
260 270 280
RIEEEEFSLI HFFGENYLEY KKKVPTGLPF IKGVKMEP
Length:288
Mass (Da):32,432
Last modified:July 1, 1997 - v1
Checksum:i7F32D5A7BC1B522B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87750 mRNA. Translation: BAA19000.1.
RefSeqiNP_001081219.1. NM_001087750.1.
UniGeneiXl.16.

Genome annotation databases

GeneIDi397717.
KEGGixla:397717.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87750 mRNA. Translation: BAA19000.1.
RefSeqiNP_001081219.1. NM_001087750.1.
UniGeneiXl.16.

3D structure databases

ProteinModelPortaliO12947.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi397717.
KEGGixla:397717.

Organism-specific databases

CTDi23463.
XenbaseiXB-GENE-6068654. icmt.

Phylogenomic databases

HOVERGENiHBG019034.
KOiK00587.

Family and domain databases

InterProiIPR007269. ICMT_MeTrfase.
IPR025770. PPMT_MeTrfase.
[Graphical view]
PfamiPF04140. ICMT. 1 hit.
[Graphical view]
PROSITEiPS51564. SAM_ICMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICMT_XENLA
AccessioniPrimary (citable) accession number: O12947
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: July 1, 1997
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.