O12947 (ICMT_XENLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase
EC=2.1.1.100

Alternative name(s):
Farnesyl cysteine carboxyl methyltransferase
Short name=FCMT
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name=PPMT
Prenylcysteine carboxyl methyltransferase
Short name=pcCMT

Gene names

Name:

icmt

Organism

Xenopus laevis (African clawed frog)

Taxonomic identifier

8355 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
Catalytic activity	S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.
Cofactor	Divalent cations. Probably zinc By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the isoprenylcysteine O-methyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Protein-S-isoprenylcysteine O-methyltransferase		PRO_0000209897
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 29	17	Helical; Potential
	Topological domain	30 – 45	16	Lumenal Potential
	Transmembrane	46 – 63	18	Helical; Potential
	Topological domain	64 – 73	10	Cytoplasmic Potential
	Transmembrane	74 – 91	18	Helical; Potential
	Topological domain	92 – 96	5	Lumenal Potential
	Transmembrane	97 – 116	20	Helical; Potential
	Topological domain	117 – 135	19	Cytoplasmic Potential
	Transmembrane	136 – 153	18	Helical; Potential
	Topological domain	154 – 158	5	Lumenal Potential
	Transmembrane	159 – 178	20	Helical; Potential
	Topological domain	179 – 216	38	Cytoplasmic Potential
	Transmembrane	217 – 232	16	Helical; Potential
	Topological domain	233	1	Lumenal Potential
	Transmembrane	234 – 248	15	Helical; Potential
	Topological domain	249 – 288	40	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	O12947 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 7F32D5A7BC1B522B	FASTA	288	32,432
	10 20 30 40 50 60 MAGARLLQEG RVSIVSFTLG ASVISLPLLT SSFTEQTLLA AAPGRIALVF FIAALNGLLL 70 80 90 100 110 120 LLYKAQLYQV AIRASFLGFA FGCGLLLSIT QSPWKPFGWY VCSLSFFHYS EYLVTAMNNP 130 140 150 160 170 180 RSLSIDSFLL NHSLEYTLAA LSSWVEFTIE TTIYPDLKQI TWLSVIGLIM VLFGEVLRKC 190 200 210 220 230 240 AMLTAGSNFN HIVQNEKSDS HTLVTSGVYS WFRHPSYVGW FYWSIGTQVL LCNPLCLVGY 250 260 270 280 TLASWRFFSE RIEEEEFSLI HFFGENYLEY KKKVPTGLPF IKGVKMEP

References

[1]	"Genes encoding farnesyl cysteine carboxyl methyltransferase in Schizosaccharomyces pombe and Xenopus laevis." Imai Y., Davey J., Kawagishi-Kobayashi M., Yamamoto M. Mol. Cell. Biol. 17:1543-1551(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovary.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87750 mRNA. Translation: BAA19000.1.
RefSeq	NP_001081219.1. NM_001087750.1.
UniGene	Xl.16.
3D structure databases
ProteinModelPortal	O12947.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	397717.
KEGG	xla:397717.
Organism-specific databases
CTD	23463.
Xenbase	XB-GENE-6068654. icmt.
Phylogenomic databases
HOVERGEN	HBG019034.
KO	K00587.
Family and domain databases
InterPro	IPR007269. ICMT_MeTrfase. IPR025770. PPMT_MeTrfase. [Graphical view]
Pfam	PF04140. ICMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ICMT_XENLA

Accession

Primary (citable) accession number: O12947

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program