ID   L_RYSV                  Reviewed;        1967 AA.
AC   O10378;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   16-JUN-2009, entry version 39.
DE   RecName: Full=Large structural protein;
DE            Short=Protein L;
DE   AltName: Full=Transcriptase;
DE   AltName: Full=Replicase;
DE   Includes:
DE     RecName: Full=RNA-directed RNA polymerase;
DE              EC=2.7.7.48;
DE   Includes:
DE     RecName: Full=mRNA (guanine-N(7)-)-methyltransferase;
DE              EC=2.1.1.56;
DE   Includes:
DE     RecName: Full=mRNA guanylyltransferase;
DE              EC=2.7.7.-;
GN   Name=L;
OS   Rice yellow stunt virus (RYSV) (Rice transitory yellowing virus).
OC   Viruses; ssRNA negative-strand viruses; Mononegavirales;
OC   Rhabdoviridae; Nucleorhabdovirus.
OX   NCBI_TaxID=59380;
OH   NCBI_TaxID=4530; Oryza sativa (Rice).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   MEDLINE=22749841; PubMed=12867659; DOI=10.1099/vir.0.19195-0;
RA   Huang Y., Zhao H., Luo Z., Chen X., Fang R.X.;
RT   "Novel structure of the genome of Rice yellow stunt virus:
RT   identification of the gene 6-encoded virion protein.";
RL   J. Gen. Virol. 84:2259-2264(2003).
CC   -!- FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl
CC       transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A)
CC       synthetase activities. The viral mRNA guanylyl transferase
CC       displays a different biochemical reaction than the cellular
CC       enzyme. The template is composed of the viral RNA tightly
CC       encapsidated by the nucleoprotein (N). Functions either as
CC       transcriptase or as replicase. The transcriptase synthesizes
CC       subsequently five subgenomic RNAs, assuring their capping and
CC       polyadenylation by a stuttering mechanism. The replicase mode is
CC       dependent on intracellular N protein concentration. In this mode,
CC       the polymerase replicates the whole viral genome without
CC       recognizing the transcriptional signals (By similarity).
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-
CC       adenosyl-L-homocysteine + m(7)G(5')pppR-RNA.
CC   -!- SUBUNIT: Interacts with the P protein (By similarity).
CC   -!- SUBCELLULAR LOCATION: Virion (Potential). Host cytoplasm (By
CC       similarity).
CC   -!- SIMILARITY: Belongs to the rhabdoviridae protein L family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; AB011257; BAA25160.1; -; Genomic_RNA.
DR   RefSeq; NP_620502.1; -.
DR   GeneID; 944307; -.
DR   BRENDA; 2.1.1.56; 305814.
DR   BRENDA; 2.7.7.48; 305814.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019012; C:virion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-directed RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006370; P:mRNA capping; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR014023; RNA_pol_cat.
DR   InterPro; IPR001016; RNA_pol_L_viral.
DR   Pfam; PF00946; Paramyx_RNA_pol; 1.
DR   PROSITE; PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Methyltransferase; mRNA capping;
KW   mRNA processing; Multifunctional enzyme; Nucleotide-binding;
KW   Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase;
KW   S-adenosyl-L-methionine; Transferase; Virion.
FT   CHAIN         1   1967       Large structural protein.
FT                                /FTId=PRO_0000297839.
FT   DOMAIN      604    790       RdRp catalytic.
SQ   SEQUENCE   1967 AA;  223602 MW;  DE70AB80A45FC776 CRC64;
     MDDEGHGYWQ DYDEDESWLD AENDVFDDDI FEEAEDNEHL VEGGDFHLKS ALRGEADMLT
     NPIYEKEREK LQEDVGDLGV ALGHLNVRPF LEKMGERIST DHTNTPLIEN IKQGGFGAIH
     HMKSTARLIV AEAATVTETM LTSGVDTTLA GAYHFFEQNH PHMKCTVNMV LMFLTILNNV
     KNIRLNVDLH QILKNNISIK GSTVCMYITV ATVAYFSTDI VVFDIDGQKY NTPKTYFLNA
     CDKIQERFNV ILYSYLAEGL SIPGSPPTYI VNRIIDWGDS ILYQMGNDGY DVIALYEATV
     VGVILSRDDK DLSPTSGGGD FLENIQQDLG PVQQNHIRYL IALLHDMTPH QLADLHGLYR
     IWAHPIIDID GGVKKLQKVT QSLKGDINSK PESQETVRSF RRLFVMDYFV KHQFYPPITL
     PEKSNCYIGN CIRTSKKIDE SHINYNFSDW DLVELGGAFS IPYSWNVLHL AKDKAISPTR
     SEMYTMLCKT KRVFNAELRR GVLKLMNTTL TPLREFLTEV AEHGLDIDDC IIGLFPKERE
     LKIMARFFAL LSFKMRLYFT ATEELLGSKL LRYFPQITMS SNLLDMQEKM SSMSRDLESQ
     NKSVTYVINM DFVKWNQQMR ESTCEGFLKN WSKLFGLPGL YSRSHQIFRD SILYIADGTR
     DLTPDPDTGI MVDNNVCWID DGAGKEGIRQ KAWTIMTVCD IAAVARHHPG VFHLVGGGDN
     QVLTVTYHTN QIDTDGNITE EGKSKIKAKV KKFIEALETH FAARGLPLKT SETWCSTSLF
     MYNKFMYYKG VPLRSPLKQV SRLFPYSNNT SMTLQSMAQC LGTGLRSAAQ KEVSHIALLF
     MRNIWGSVLG WIILYCHPML PSISSETCNS GVSTIVRGRK AINMKTHRID LVALILKILY
     LPGQLGGPGL VNIYQMTMRG FPDPVTEAIC FLRKFKNYLI CVGSSYSSHL ARMAGVSFSA
     SRSYEPLIED VCSLNLDTPR SGTGGKREVP RKILLKSRLG GNQHLKELLG IMKGPSEGEF
     YRAISSGKVL DVRVMHEITS STLYAVTNTF TSRVDKTATL KRLTFKFSML ESLADAELKY
     IRYLSVRDDK THDIMFDGCS RIIADECRTK GWGKPVLGVT VPTPFEYLQI SWTDEHICDN
     NHITVRISNQ DRQVTTETLG PCKPYLGAYT KEKFKMTEVA AAYGDEDVLS KSLRILKIIN
     WRYQDGSTMS EIIKAPFRAV TDIDPQRMVQ ESTVTKGDYD HRRKMDARVH GGIPNFVTTP
     LSHISISTST WYKHARGKNE NIHFQACIIQ TMYQIIIRTM SNIHSQEKLH VHECCQTSIS
     EIQEPRANLD LTTPQMIPTF PTLMGNPLVY IPEQSITFDY SRTQEVDYSR RVGSLDCQRS
     TDYGWVDAYS SLSWLIMCDV IGWTKMPDSF YIMQQERVDH YLLSMYIISI WKVLKSEFDL
     HKTMLDWGPL LKVYSSDTGV QVLSQSMGIV VLGKLEGGLS VNMMDICNSL TGLTSNQIPP
     FSVNLALPRI HKQVATWWKL TCSDTMYCIQ CSNILKGYWE GTLINTRIDN DLRCSECADG
     GISPRIVNAH ISNLSDHLIK IIPDREEVQL PLLAGGEDLN NLEVVLSLED EWPDSDDITI
     LNRIGEMNDI DGRMKNYLEV MALLNPDVVI IRPEILELEI LRRVCEVVRL ASEGKLIIHI
     LVEEPSYLES GAIYEMERAF PRHNLFHVQF SFRKPPEGLH KLWLLPQDIT LARADVGDWL
     VIPFTQLLDF HDTITPDTRL HHQREAIRQD VFWSTKEQGG LGLVVLQHIC TKTELSYRLD
     RSKVDVEINK MMIKDPVMGG KYVILKRRCS WAIFSTKYEL LCSAERIKGN LSADGKKITS
     KDFSRTYKED IIIFIISVLM RSLDCDEKRV MTLSYVECLP ELLTLKPRFN KSGVISVRYL
     DYFWFFKRVY SYNNPEVAIP YSAVIKGVNL GSPRKEGYSL ASSDGAP
//