O10378 (L_RYSV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Rice yellow stunt virus (RYSV) (Rice transitory yellowing virus) [Complete proteome]

Taxonomic identifier

59380 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Nucleorhabdovirus

Virus host

Oryza sativa (Rice) [TaxID: 4530]

Protein attributes

Sequence length	1967 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1967	1967	RNA-directed RNA polymerase L		PRO_0000297839
Regions
	Domain	604 – 790	187	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	O10378 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: DE70AB80A45FC776	FASTA	1,967	223,602
	10 20 30 40 50 60 MDDEGHGYWQ DYDEDESWLD AENDVFDDDI FEEAEDNEHL VEGGDFHLKS ALRGEADMLT 70 80 90 100 110 120 NPIYEKEREK LQEDVGDLGV ALGHLNVRPF LEKMGERIST DHTNTPLIEN IKQGGFGAIH 130 140 150 160 170 180 HMKSTARLIV AEAATVTETM LTSGVDTTLA GAYHFFEQNH PHMKCTVNMV LMFLTILNNV 190 200 210 220 230 240 KNIRLNVDLH QILKNNISIK GSTVCMYITV ATVAYFSTDI VVFDIDGQKY NTPKTYFLNA 250 260 270 280 290 300 CDKIQERFNV ILYSYLAEGL SIPGSPPTYI VNRIIDWGDS ILYQMGNDGY DVIALYEATV 310 320 330 340 350 360 VGVILSRDDK DLSPTSGGGD FLENIQQDLG PVQQNHIRYL IALLHDMTPH QLADLHGLYR 370 380 390 400 410 420 IWAHPIIDID GGVKKLQKVT QSLKGDINSK PESQETVRSF RRLFVMDYFV KHQFYPPITL 430 440 450 460 470 480 PEKSNCYIGN CIRTSKKIDE SHINYNFSDW DLVELGGAFS IPYSWNVLHL AKDKAISPTR 490 500 510 520 530 540 SEMYTMLCKT KRVFNAELRR GVLKLMNTTL TPLREFLTEV AEHGLDIDDC IIGLFPKERE 550 560 570 580 590 600 LKIMARFFAL LSFKMRLYFT ATEELLGSKL LRYFPQITMS SNLLDMQEKM SSMSRDLESQ 610 620 630 640 650 660 NKSVTYVINM DFVKWNQQMR ESTCEGFLKN WSKLFGLPGL YSRSHQIFRD SILYIADGTR 670 680 690 700 710 720 DLTPDPDTGI MVDNNVCWID DGAGKEGIRQ KAWTIMTVCD IAAVARHHPG VFHLVGGGDN 730 740 750 760 770 780 QVLTVTYHTN QIDTDGNITE EGKSKIKAKV KKFIEALETH FAARGLPLKT SETWCSTSLF 790 800 810 820 830 840 MYNKFMYYKG VPLRSPLKQV SRLFPYSNNT SMTLQSMAQC LGTGLRSAAQ KEVSHIALLF 850 860 870 880 890 900 MRNIWGSVLG WIILYCHPML PSISSETCNS GVSTIVRGRK AINMKTHRID LVALILKILY 910 920 930 940 950 960 LPGQLGGPGL VNIYQMTMRG FPDPVTEAIC FLRKFKNYLI CVGSSYSSHL ARMAGVSFSA 970 980 990 1000 1010 1020 SRSYEPLIED VCSLNLDTPR SGTGGKREVP RKILLKSRLG GNQHLKELLG IMKGPSEGEF 1030 1040 1050 1060 1070 1080 YRAISSGKVL DVRVMHEITS STLYAVTNTF TSRVDKTATL KRLTFKFSML ESLADAELKY 1090 1100 1110 1120 1130 1140 IRYLSVRDDK THDIMFDGCS RIIADECRTK GWGKPVLGVT VPTPFEYLQI SWTDEHICDN 1150 1160 1170 1180 1190 1200 NHITVRISNQ DRQVTTETLG PCKPYLGAYT KEKFKMTEVA AAYGDEDVLS KSLRILKIIN 1210 1220 1230 1240 1250 1260 WRYQDGSTMS EIIKAPFRAV TDIDPQRMVQ ESTVTKGDYD HRRKMDARVH GGIPNFVTTP 1270 1280 1290 1300 1310 1320 LSHISISTST WYKHARGKNE NIHFQACIIQ TMYQIIIRTM SNIHSQEKLH VHECCQTSIS 1330 1340 1350 1360 1370 1380 EIQEPRANLD LTTPQMIPTF PTLMGNPLVY IPEQSITFDY SRTQEVDYSR RVGSLDCQRS 1390 1400 1410 1420 1430 1440 TDYGWVDAYS SLSWLIMCDV IGWTKMPDSF YIMQQERVDH YLLSMYIISI WKVLKSEFDL 1450 1460 1470 1480 1490 1500 HKTMLDWGPL LKVYSSDTGV QVLSQSMGIV VLGKLEGGLS VNMMDICNSL TGLTSNQIPP 1510 1520 1530 1540 1550 1560 FSVNLALPRI HKQVATWWKL TCSDTMYCIQ CSNILKGYWE GTLINTRIDN DLRCSECADG 1570 1580 1590 1600 1610 1620 GISPRIVNAH ISNLSDHLIK IIPDREEVQL PLLAGGEDLN NLEVVLSLED EWPDSDDITI 1630 1640 1650 1660 1670 1680 LNRIGEMNDI DGRMKNYLEV MALLNPDVVI IRPEILELEI LRRVCEVVRL ASEGKLIIHI 1690 1700 1710 1720 1730 1740 LVEEPSYLES GAIYEMERAF PRHNLFHVQF SFRKPPEGLH KLWLLPQDIT LARADVGDWL 1750 1760 1770 1780 1790 1800 VIPFTQLLDF HDTITPDTRL HHQREAIRQD VFWSTKEQGG LGLVVLQHIC TKTELSYRLD 1810 1820 1830 1840 1850 1860 RSKVDVEINK MMIKDPVMGG KYVILKRRCS WAIFSTKYEL LCSAERIKGN LSADGKKITS 1870 1880 1890 1900 1910 1920 KDFSRTYKED IIIFIISVLM RSLDCDEKRV MTLSYVECLP ELLTLKPRFN KSGVISVRYL 1930 1940 1950 1960 DYFWFFKRVY SYNNPEVAIP YSAVIKGVNL GSPRKEGYSL ASSDGAP

References

[1]	"Novel structure of the genome of Rice yellow stunt virus: identification of the gene 6-encoded virion protein." Huang Y., Zhao H., Luo Z., Chen X., Fang R.X. J. Gen. Virol. 84:2259-2264(2003) [PubMed: 12867659] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011257 Genomic RNA. Translation: BAA25160.1.
RefSeq	NP_620502.1. NC_003746.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	944307.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_RYSV

Accession

Primary (citable) accession number: O10378

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	July 1, 1997
Last modified:	December 14, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program