O10363 (CHIT_NPVOP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable endochitinase
EC=3.2.1.14

Gene names

ORF Names:

ORF124

Organism

Orgyia pseudotsugata multicapsid polyhedrosis virus (OpMNPV) [Complete proteome]

Taxonomic identifier

262177 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Baculoviridae › Alphabaculovirus

Virus host

Orgyia pseudotsugata (Douglas fir tussock moth) [TaxID: 33414]

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Host endoplasmic reticulum lumen Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Cellular component	Host endoplasmic reticulum
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 550	534	Probable endochitinase		PRO_0000011951
Regions
	Motif	547 – 550	4	Prevents secretion from ER Potential
Sites
	Active site	304	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	146	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	172	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	344	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	O10363 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 77947F5CF00E07BD	FASTA	550	60,734
	10 20 30 40 50 60 MLHYLATILW LAVAHASPGT PVIDWADRNY ALVSVNSEAT AYENLVERKA GVSVPVSWNV 70 80 90 100 110 120 WNGGVGDMAY VLFNENQVWK GAAAAKRATI DVSKSGQFNM RVKLCDDDGF SVSEPVTVRV 130 140 150 160 170 180 ADTDGGHLSP LEYAWGENNK PGRPHNKTVA AYFVEWGVYG RGFPVDKVPL PNLSHLLYGF 190 200 210 220 230 240 IPICGGDGLN DALKTIPGSF EALQRSCKGR ADFKVAIHDP WAAIQKPQKG VSAWNEPYKG 250 260 270 280 290 300 NFGQLMAAKL ANPHLKILPS IGGWTLSDPF YFMHDADKRR VFVESVKEFL QVWKFFDGVD 310 320 330 340 350 360 IDWEFPGGKG ANPALGNGER DADTYLVLLK ELRAMLDELQ LQTNKTYELT SAISSGYDKI 370 380 390 400 410 420 AVVKYDAAQR FLDKIFLMSY DFKGAWSNTD LGYQTTLYAP SWNANELYTT DHAVKLLTGQ 430 440 450 460 470 480 GVAAHKLIVG VAMYGRGWTG VSGYAGDKYF SGTADGPVPG TWENGVVDYR QINNELSKYI 490 500 510 520 530 540 YRFDAAAKAA YVFNKERGDL ISFDSVDSVL AKNVYVQQNG LGGLFAWEID ADNGDLLNAM 550 NERVRVKDEL

References

[1]	"The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome." Ahrens C.H., Russell R.R., Funk C.J., Evans J., Harwood S., Rohrmann G.F. Virology 229:381-399(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U75930 Genomic DNA. Translation: AAC59123.1.
RefSeq	NP_046280.1. NC_001875.2.
3D structure databases
ProteinModelPortal	O10363.
SMR	O10363. Positions 16-541.
ModBase	Search...
Protein family/group databases
CAZy	GH18. Glycoside Hydrolase Family 18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	912030.
Phylogenomic databases
ProtClustDB	CLSP2509983.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 3.20.20.80. 2 hits.
InterPro	IPR011583. Chitinase_II. IPR013540. ChitinaseA_N. IPR001223. Glyco_hydro18cat. IPR001579. Glyco_hydro_18_chit_AS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022409. PKD/Chitinase_dom. [Graphical view]
Pfam	PF08329. ChitinaseA_N. 1 hit. PF00704. Glyco_hydro_18. 1 hit. [Graphical view]
SMART	SM00636. Glyco_18. 1 hit. SM00089. PKD. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
PROSITE	PS01095. CHITINASE_18. 1 hit. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIT_NPVOP

Accession

Primary (citable) accession number: O10363

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program