O09705 (L_LASSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) (LASV)

Taxonomic identifier

11622 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › Old world arenaviruses

Virus host

Homo sapiens (Human) [TaxID: 9606]
Praomys natalensis (African soft-furred rat) (Mastomys natalensis) [TaxID: 10112]

Protein attributes

Sequence length	2218 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Cap snatching RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2218	2218	RNA-directed RNA polymerase L		PRO_0000361639
Regions
	Domain	1174 – 1370	197	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	O09705 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: F325C51F96ED2EA6	FASTA	2,218	253,432
	10 20 30 40 50 60 MEEDIACVKD LVSKYLVDNE RLSRQKLAFL VQTEPRMLLM EGLKLLSLCI EVDSCNANGC 70 80 90 100 110 120 EHNSEDKSVE RILHDHGILT PSLCLWYPDG YKLTGNVLIL LECFVRSSPA NFEQKYIEDF 130 140 150 160 170 180 KKLEQLKEDL KSVDINLIPL IDGRTSFYNE QIPDWVNDKL RDTLFSLLKY AQESNSLFEE 190 200 210 220 230 240 SEYSRLCESL FMTSGRLSGV ESLNVLMDNR SNHYEEVIAS CHQGINNKLT AHEVKLQIEE 250 260 270 280 290 300 EYQVFRNRLR KGEIEGQFLK VDKSQLLNEL NNLYADKVVA EDNIEHLIYQ FKRASPILRF 310 320 330 340 350 360 LYANVDEGNE KRGNQTIGEC QVQCWRSFLN KVKSLRILNT RRKLLLIFDA LILLASKHDL 370 380 390 400 410 420 MKQKCLKGWL GSCFLRVKDR LVSLEATKRD LEKWGERGNR LRSRITQSSQ CLSKNQILNS 430 440 450 460 470 480 IFQKTILKAT TALKDVGISV DHYKIDMEVI CLNSYDLIMD FDVSGVVPTI SYQRTEEETF 490 500 510 520 530 540 PYVMGDVELL GTTDLERLSS LSLALVNSMK TSSTVKLRQN EFGPARYQVV RCKEAYCQEF 550 560 570 580 590 600 SLGNTELQLI YQKTGECSKC YAINDNKVGE VCSFYADPKR YFPAIFSAEV LQTTISTMIS 610 620 630 640 650 660 WIEDCNELEG QLNNIRSLTK MILVLILAHP SKRSQKLLQN LRYFVMAYLS DYHHKDLIDK 670 680 690 700 710 720 IREELITDVE FLLYRLIRTL MNLVLSEDVK SMMTNRFKFI LNVSYMCHFI TKETPDRLTD 730 740 750 760 770 780 QIKCFEKFLE PKVRFGHVST NPADTATEEE LDDMVYNAKK FLSKDGCTTI EGPDYKRPGV 790 800 810 820 830 840 SKKYLSLLTS SFNNGSLFKE REVKREIKDP LITSGSAALD LASKKSVVVN KYTDGSRILN 850 860 870 880 890 900 YDFNKLTALA VSQLTEVFSR KGKYLLNKQD YEYKVQQAMS NLVLGSGQLK SDADGADLDE 910 920 930 940 950 960 ILLDGGASDY FDQLKETVEK IVDQYREPVK LGSGPNGDGQ PSINDLDEIV SNKFYIRLIK 970 980 990 1000 1010 1020 GELSNHMVEE FDHDILPGKF YEEFCNAVYE NSRLKQKYFY CGHMSQCPIG ELTKAVSTRT 1030 1040 1050 1060 1070 1080 YFNHEYFQCF KSILLIMNAN TLMGRYTHYK SRNLNFKFDM GKLSDDVRIS ERESNSEALS 1090 1100 1110 1120 1130 1140 KALSLTNCTT AMLKNLCFYS QESPQSYDSV GPDTGRLKFS LSYKEQVGGN RELYIGDLRT 1150 1160 1170 1180 1190 1200 KMFTRLIEDY FEAISLQLSG SCLNNEKEFE NAILSMKLNV SLAHVSYSMD HSKWGPMMCP 1210 1220 1230 1240 1250 1260 FLFLTVLQNL IFLSKDLQAD IKGRDYLSTL LMWHMHKMVE IPFNVVTAMM KSFIKAQLGL 1270 1280 1290 1300 1310 1320 RKKTKQSITE DFFYSNFQAG VVPSHISSIL DMGQGILHNT SDFYALISER FINYAISCIC 1330 1340 1350 1360 1370 1380 GGTIDAYTSS DDQISLFDQS LTELLQRDPE EFRTLIEFHY YMSDQLNKFV SPKSVIGRFV 1390 1400 1410 1420 1430 1440 AEFKSRFFVW GDEVPLLTKF VAAALHNIKC KEPHQLAETI DTIIDQSVAN GVPVHLCNLI 1450 1460 1470 1480 1490 1500 QKRTLNPLQY ARYPIDPFLL NCETDVRDWV DGNRSYRIMR QIEGLIPNAC SKIRSMLRKL 1510 1520 1530 1540 1550 1560 YNRLKTGQLH EEFTTNYLSS EHLSSLRNLC ELLDVEPPSE SDLEYSWLNL AAHHPLRMVL 1570 1580 1590 1600 1610 1620 RQKIIYSGAV NLDDEKIPTI VKTIQNKLSS TFTRGAQKLL SEAINKSAFQ SSIASGFVGL 1630 1640 1650 1660 1670 1680 CRTLGSKCVR GPNKENLYIK SIQSLISDVK GIKLLTNSNG IQYWQVPLEL RNGSGGESVV 1690 1700 1710 1720 1730 1740 SYFRPLLWDY MCISLSTAIE LGAWVLGEPK TVKVFDFFKH NPCDYFPLKP TASKLLEDRV 1750 1760 1770 1780 1790 1800 GLNHIIHSLR RLYPSVFEKH ILPFMSDLAS TKMKWSPRIK FLDLCVALDV NCEALSLVSH 1810 1820 1830 1840 1850 1860 IVKWKREEHY IVLSSELRLS HSRTHEPMVE ERVVSTSDAV DNFMRQIYFE SYVRPFVATT 1870 1880 1890 1900 1910 1920 RTLGSFTWFP HKTSVPEGEG LHRMGPFSSF VEKVIHKGVE RPMFKHDLMM GYAWIDFDIE 1930 1940 1950 1960 1970 1980 PARFNQNQLI ASGLVDPKFD SLEDFFDAVA SLPPGSAKLS QTVRFRVKSQ DASFKESFAI 1990 2000 2010 2020 2030 2040 HLEYTGSMNQ QAKYLVHDVT VMYSGAVSPC VLSDCWRLVL SGPTFKGKSA WYVDTEIINE 2050 2060 2070 2080 2090 2100 FLIDTNQLGH VTPVEIVVDM ERLQFTEYDF VLVGPCTEPT PLVVHRGGLW ECGKKLASFT 2110 2120 2130 2140 2150 2160 PVIQDQDLEI FVREVGDTSS DLLIGALSDM MIDRLGLRMQ WSGVDIVSTL RAAAPSCEGI 2170 2180 2190 2200 2210 LSAVLEAVDN WVEFKGYALC YSKSRGKVMV QSSGGKLRLK GRTCEELTRK DECIEDIE

References

[1]	"The Lassa fever virus L gene: nucleotide sequence, comparison, and precipitation of a predicted 250 kDa protein with monospecific antiserum." Lukashevich I.S., Djavani M., Shapiro K., Sanchez A., Ravkov E., Nichol S.T., Salvato M.S. J. Gen. Virol. 78:547-551(1997) [PubMed: 9049403] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Completion of the Lassa fever virus sequence and identification of a RING finger open reading frame at the L RNA 5' End." Djavani M., Lukashevich I.S., Sanchez A., Nichol S.T., Salvato M.S. Virology 235:414-418(1997) [PubMed: 9281522] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	U63094 Genomic RNA. Translation: AAB50401.1. U73034 Genomic RNA. Translation: AAC05817.1.
RefSeq	NP_694872.1. NC_004297.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	956587.
Family and domain databases
InterPro	IPR014386. RNA-dir_pol_arenavirus. IPR007099. RNA-dir_pol_NSvirus. IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000836. L_ArenaV. 1 hit.
PROSITE	PS50525. RDRP_SSRNA_NEG_SEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_LASSJ

Accession

Primary (citable) accession number: O09705

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	July 1, 1997
Last modified:	September 21, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program