Skip Header

Contribute Send feedback
Read comments (?) or add your own

O09460 (PCKA_ANASU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 31, 2011. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [ATP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.49
Alternative name(s):
Phosphoenolpyruvate carboxylase
Gene names
Name:pckA
OrganismAnaerobiospirillum succiniciproducens
Taxonomic identifier13335 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Succinivibrionaceae › Anaerobiospirillum

Protein attributes

Sequence length532 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2. HAMAP MF_00453

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00453

Subcellular location

Cytoplasm By similarity HAMAP MF_00453.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical term3D-structure
Gene Ontology (GO)
   Biological processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoenolpyruvate carboxykinase (ATP) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 532532Phosphoenolpyruvate carboxykinase [ATP] HAMAP MF_00453
PRO_0000203801

Regions

Nucleotide binding242 – 2498ATP By similarity

Secondary structure

1 .......................................................................................................... 532
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O09460 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 983ABC71930F9E44

FASTA53258,643
        10         20         30         40         50         60 
MSLSESLAKY GITGATNIVH NPSHEELFAA ETQASLEGFE KGTVTEMGAV NVMTGVYTGR 

        70         80         90        100        110        120 
SPKDKFIVKN EASKEIWWTS DEFKNDNKPV TEEAWAQLKA LAGKELSNKP LYVVDLFCGA 

       130        140        150        160        170        180 
NENTRLKIRF VMEVAWQAHF VTNMFIRPTE EELKGFEPDF VVLNASKAKV ENFKELGLNS 

       190        200        210        220        230        240 
ETAVVFNLAE KMQIILNTWY GGEMKKGMFS MMNFYLPLQG IAAMHCSANT DLEGKNTAIF 

       250        260        270        280        290        300 
FGLSGTGKTT LSTDPKRLLI GDDEHGWDDD GVFNFEGGCY AKVINLSKEN EPDIWGAIKR 

       310        320        330        340        350        360 
NALLENVTVD ANGKVDFADK SVTENTRVSY PIFHIKNIVK PVSKAPAAKR VIFLSADAFG 

       370        380        390        400        410        420 
VLPPVSILSK EQTKYYFLSG FTAKLAGTER GITEPTPTFS SCFGAAFLTL PPTKYAEVLV 

       430        440        450        460        470        480 
KRMEASGAKA YLVNTGWNGT GKRISIKDTR GIIDAILDGS IDTANTATIP YFNFTVPTEL 

       490        500        510        520        530 
KGVDTKILDP RNTYADASEW EVKAKDLAER FQKNFKKFES LGGDLVKAGP QL

« Hide

References

[1]"Cloning, sequencing, and overexpression of the Anaerobiospirillum succiniciproducens phosphoenolpyruvate carboxykinase (pckA) gene."
Laivenieks M., Vieille C., Zeikus J.G.
Appl. Environ. Microbiol. 63:2273-2280(1997) [PubMed: 9172347] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 29305 / DSM 6400 / LMG 7826 / NCTC 11536.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U95960 Genomic DNA. Translation: AAC45394.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1YTMX-ray2.20A/B1-532[»]
1YVYX-ray2.35A/B1-532[»]
ProteinModelPortalO09460.
SMRO09460. Positions 2-518.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_00453. PEPCK_ATP.
[Tree]
InterProIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
Gene3DG3DSA:3.90.228.20. PEP_carboxykinase_C. 2 hits.
G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
PfamPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMSSF68923. PEP_carboxykinase_N. 1 hit.
TIGRFAMsTIGR00224. PckA. 1 hit.
PROSITEPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKA_ANASU
AccessionPrimary (citable) accession number: O09460
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: May 31, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents