ID   G3PA_GUITH              Reviewed;         386 AA.
AC   O09452;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   16-JUN-2009, entry version 57.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase, chloroplastic;
DE            EC=1.2.1.59;
DE   AltName: Full=NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase;
DE   Flags: Precursor;
GN   Name=GAPC1;
OS   Guillardia theta (Cryptomonas phi).
OC   Eukaryota; Cryptophyta; Pyrenomonadales; Geminigeraceae; Guillardia.
OX   NCBI_TaxID=55529;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=97224625; PubMed=9071009; DOI=10.1007/PL00000050;
RA   Liaud M.-F., Brandt U., Scherzinger M., Cerff R.;
RT   "Evolutionary origin of cryptomonad microalgae: two novel
RT   chloroplast/cytosol-specific GAPDH genes as potential markers of
RT   ancestral endosymbiont and host cell components.";
RL   J. Mol. Evol. 44:S28-S37(1997).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
CC   -!- PATHWAY: Carbohydrate biosynthesis; Calvin cycle.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; U40032; AAC49702.1; -; mRNA.
DR   HSSP; P06977; 1DC5.
DR   BRENDA; 1.2.1.59; 279957.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0043891; F:glyceraldehyde-3-phosphate dehydrogenase (N...; IEA:EC.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006006; P:glucose metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0019253; P:reductive pentose-phosphate cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   2: Evidence at transcript level;
KW   Calvin cycle; Chloroplast; NAD; NADP; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT       1     45       Chloroplast (By similarity).
FT   CHAIN        46    386       Glyceraldehyde-3-phosphate dehydrogenase,
FT                                chloroplastic.
FT                                /FTId=PRO_0000010421.
FT   NP_BIND      58     59       NADP (By similarity).
FT   REGION      197    199       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      257    258       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    198    198       Nucleophile (By similarity).
FT   BINDING     125    125       NADP; via carbonyl oxygen (By
FT                                similarity).
FT   BINDING     228    228       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     280    280       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     362    362       NADP (By similarity).
FT   SITE        225    225       Activates thiol group during catalysis
FT                                (By similarity).
SQ   SEQUENCE   386 AA;  41426 MW;  1EFC9FB8CA260569 CRC64;
     MAYFKAVAYL AALASAAAFN PGSSFVPRLN APATQPKAAK MTGPTMQAVP CGINGFGRIG
     RLVARIMIKD PETKLLQVNA GSATPDYMAY QFKYDSIHGR YQGDVVVDGD DLVIDGQRVI
     TTRARDPKDI GWAKTGVEYL CESTGVFLTA EKAQPHIDAG VKKVIFSAPA KDDSLTVVMG
     VNAEDYKGQT DFISCASCTT NGLAPLVKCI NDKWGIEEGL MTTIHAMTAT QAVVDSSSRK
     DWRGGRAASG NIIPSSTGAA KAVAKVIPAV KGKLTGMAFR VPTIDVSVVD LTCRLAKDAT
     YEEICAEVKR RANGDMKGFL GYTDEPLVST DFETEPISST FDAEAGISLN PRFVKLVAWY
     DNEWGYSNRV KDLMVHVAKV DAKVKA
//