Reviewed, UniProtKB/Swiss-Prot O09452 (G3PA_GUITH)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase, chloroplastic
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:

GAPC1

Organism

Guillardia theta (Cryptomonas phi)

Taxonomic identifier

55529 [NCBI]

Taxonomic lineage

Eukaryota › Cryptophyta › Pyrenomonadales › Geminigeraceae › Guillardia

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 45	45	Chloroplast By similarity
	Chain	46 – 386	341	Glyceraldehyde-3-phosphate dehydrogenase, chloroplastic		PRO_0000010421
Regions
	Nucleotide binding	58 – 59	2	NADP By similarity
	Region	197 – 199	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	257 – 258	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	198	1	Nucleophile By similarity
	Binding site	125	1	NADP; via carbonyl oxygen By similarity
	Binding site	228	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	280	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	362	1	NADP By similarity
	Site	225	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	O09452-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 1EFC9FB8CA260569	FASTA	386	41,426
	10 20 30 40 50 60 MAYFKAVAYL AALASAAAFN PGSSFVPRLN APATQPKAAK MTGPTMQAVP CGINGFGRIG 70 80 90 100 110 120 RLVARIMIKD PETKLLQVNA GSATPDYMAY QFKYDSIHGR YQGDVVVDGD DLVIDGQRVI 130 140 150 160 170 180 TTRARDPKDI GWAKTGVEYL CESTGVFLTA EKAQPHIDAG VKKVIFSAPA KDDSLTVVMG 190 200 210 220 230 240 VNAEDYKGQT DFISCASCTT NGLAPLVKCI NDKWGIEEGL MTTIHAMTAT QAVVDSSSRK 250 260 270 280 290 300 DWRGGRAASG NIIPSSTGAA KAVAKVIPAV KGKLTGMAFR VPTIDVSVVD LTCRLAKDAT 310 320 330 340 350 360 YEEICAEVKR RANGDMKGFL GYTDEPLVST DFETEPISST FDAEAGISLN PRFVKLVAWY 370 380 DNEWGYSNRV KDLMVHVAKV DAKVKA

References

[1]

"Evolutionary origin of cryptomonad microalgae: two novel chloroplast/cytosol-specific GAPDH genes as potential markers of ancestral endosymbiont and host cell components." Liaud M.-F., Brandt U., Scherzinger M., Cerff R. J. Mol. Evol. 44:S28-S37(1997) [PubMed: 9071009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U40032 mRNA. Translation: AAC49702.1.
3D structure databases
HSSP	HSSP built from PDB template 1DC5 based on UniProtKB P06977.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.59. 279957.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PA_GUITH

Accession

Primary (citable) accession number: O09452

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)