Reviewed, UniProtKB/Swiss-Prot O09178 (AMPD3_RAT)

Names and origin

Protein names

Recommended name:
    AMP deaminase 3
    EC=3.5.4.6
Alternative name(s):
    AMP deaminase isoform E

Gene names

Name:

Ampd3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	765 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	AMP deaminase plays a critical role in energy metabolism.
Catalytic activity	AMP + H2O = IMP + NH3.
Pathway	Purine metabolism; IMP biosynthesis via salvage pathway; IMP from AMP: step 1/1.
Subunit structure	Homotetramer By similarity.
Tissue specificity	Three isoforms are present in mammals: AMP deaminase 1 is the predominant form in skeletal muscle; AMP deaminase 2 predominates in smooth muscle, non-muscle tissue, embryonic muscle and undifferentiated myoblasts; AMP deaminase 3 is found in erythrocytes By similarity.
Sequence similarities	Belongs to the adenosine and AMP deaminases family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	AMP catabolic process Ref.1 Traceable author statement. Source: RGD purine base metabolic process Inferred from electronic annotation. Source: InterPro purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP deaminase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced.

Isoform 1 (identifier: O09178-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 765	765	AMP deaminase 3		PRO_0000194412

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: F826FA5966221B09	FASTA	765	88,483
	10 20 30 40 50 60 MPRQFPKLNM SDLDEHVRLL AEKVFAKVLR EEDSKDVMSL FTVPKDCPIG QKEAKERELQ 70 80 90 100 110 120 KELAEQKSVE TAKRKKSFKM IRSQSMSLQM PTQDWKGPPS VSPAMSPTTP LVLGAASKPG 130 140 150 160 170 180 LAPYDMPEYQ RATISGDYCA GITMEDYEQA AKSLAKALMI REKYARLAYH RFPRTTAQYL 190 200 210 220 230 240 AHQGESVPLE EGLPDFHPPP LPQEDPYCLD DAPPNLGYLV RMQGGVLFVY DNQTMLERQE 250 260 270 280 290 300 PHSLPYPDLE TYIVDMSHIL ALITDGPTKT YCHRRLNFLE SKFSLHEMLN EMSEFKELKS 310 320 330 340 350 360 NPHRDFYNVR KVDTHIHAAA CMNQKHLLRF IKYTYQTEPD RTVAEKLGRK ITLRQVFDSL 370 380 390 400 410 420 HMDPYDLTVD SLDVHAGRQT FHGFDKFNSK YNPVGASELR DLYLKTENYL GGEYFARMVK 430 440 450 460 470 480 EVARELEDSK YQYSEPRLSI YGRSPKEWSS LARWFIQHKV YSPNMRWIIQ VPRIYDIFRS 490 500 510 520 530 540 KKLLPSFGKM LENIFLPLFQ ATINPQDHRE LHLFLKYVTG FDSVDDESKH SDHMFSDKSP 550 560 570 580 590 600 SPDLWTSEQN PPYSYYLYYM YANIMVLNNL RRERGLSTFL FRPHCGEAGS ITHLVSAFLT 610 620 630 640 650 660 ADNISHGLLL KKSPVLQYLY YLAQIPIAMS PLSNNSLFLE YSKNPLREFL HKGLHVSLST 670 680 690 700 710 720 DDPMQFHYTK EALMEEYAIA AQVWKLSTCD LCEIARNSVL QSGLSHQEKQ KFLGQNYYKE 730 740 750 760 GPEGNDIRKT NVAQIRMAFR YETLCNELSF LSDAMKSEEI TALAD

References

[1]	"Regulation of rat AMP deaminase 3 (isoform C) by development and skeletal muscle fibre type." Mahnke-Zizelman D.K., D'Cunha J., Wojnar J.M., Brogley M.A., Sabina R.L. Biochem. J. 326:521-529(1997) [PubMed: 9291127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U90888 mRNA. Translation: AAC53348.1. Different initiation.
IPI	IPI00231195.
RefSeq	NP_113732.1.
UniGene	Rn.11106
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O09178.
Genome annotation databases
Ensembl	ENSRNOT00000024933; ENSRNOP00000024933; ENSRNOG00000018262; Rattus norvegicus. [Genome view]
GeneID	25095.
KEGG	rno:25095.
UCSC	U90888. rat.
Organism-specific databases
CTD	25095.
RGD	2111. Ampd3.
Phylogenomic databases
HOVERGEN	O09178.
Enzyme and pathway databases
BRENDA	3.5.4.6. 248.
Gene expression databases
ArrayExpress	O09178.
Genevestigator	O09178.
GermOnline	ENSRNOG00000018262. Rattus norvegicus.
Family and domain databases
InterPro	IPR006650. A/AMP_deam_AS. IPR001365. A/AMP_deaminase. IPR006329. AMP_deaminase. IPR016297. AMP_deaminase_met. [Graphical view]
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
PIRSF	PIRSF001251. AMP_deaminase_met. 1 hit.
TIGRFAMs	TIGR01429. AMP_deaminase. 1 hit.
PROSITE	PS00485. A_DEAMINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	605393.

Entry information

Entry name

AMPD3_RAT

Accession

Primary (citable) accession number: O09178

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	October 13, 2009
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)