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Protein

AMP deaminase 3

Gene

Ampd3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

AMP deaminase plays a critical role in energy metabolism.

Catalytic activityi

AMP + H2O = IMP + NH3.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from AMP.
Proteins known to be involved in this subpathway in this organism are:
  1. AMP deaminase 1 (Ampd1), AMP deaminase 2 (Ampd2), AMP deaminase 3 (Ampd3)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from AMP, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi315Zinc; catalyticBy similarity1
Metal bindingi317Zinc; catalyticBy similarity1
Binding sitei317SubstrateBy similarity1
Metal bindingi584Zinc; catalyticBy similarity1
Binding sitei587SubstrateBy similarity1
Active sitei606Proton acceptorPROSITE-ProRule annotation1
Metal bindingi661Zinc; catalyticBy similarity1

GO - Molecular functioni

  • AMP deaminase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • AMP catabolic process Source: RGD
  • IMP salvage Source: UniProtKB-UniPathway

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00591; UER00663

Names & Taxonomyi

Protein namesi
Recommended name:
AMP deaminase 3 (EC:3.5.4.6)
Alternative name(s):
AMP deaminase isoform E
Gene namesi
Name:Ampd3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2111 Ampd3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001944121 – 765AMP deaminase 3Add BLAST765

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei85PhosphoserineBy similarity1
Modified residuei106PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO09178
PRIDEiO09178

PTM databases

iPTMnetiO09178
PhosphoSitePlusiO09178

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024933

Chemistry databases

BindingDBiO09178

Structurei

3D structure databases

ProteinModelPortaliO09178
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni386 – 391Substrate bindingBy similarity6
Regioni662 – 665Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1096 Eukaryota
COG1816 LUCA
HOGENOMiHOG000092200
HOVERGENiHBG050494
InParanoidiO09178
KOiK01490
PhylomeDBiO09178

Family and domain databases

CDDicd01319 AMPD, 1 hit
InterProiView protein in InterPro
IPR006650 A/AMP_deam_AS
IPR001365 A/AMP_deaminase_dom
IPR006329 AMPD
IPR029771 AMPD3
IPR032466 Metal_Hydrolase
PANTHERiPTHR11359 PTHR11359, 1 hit
PTHR11359:SF2 PTHR11359:SF2, 1 hit
PfamiView protein in Pfam
PF00962 A_deaminase, 1 hit
PIRSFiPIRSF001251 AMP_deaminase_met, 1 hit
SUPFAMiSSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR01429 AMP_deaminase, 1 hit
PROSITEiView protein in PROSITE
PS00485 A_DEAMINASE, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: O09178-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRQFPKLNM SDLDEHVRLL AEKVFAKVLR EEDSKDVMSL FTVPKDCPIG
60 70 80 90 100
QKEAKERELQ KELAEQKSVE TAKRKKSFKM IRSQSMSLQM PTQDWKGPPS
110 120 130 140 150
VSPAMSPTTP LVLGAASKPG LAPYDMPEYQ RATISGDYCA GITMEDYEQA
160 170 180 190 200
AKSLAKALMI REKYARLAYH RFPRTTAQYL AHQGESVPLE EGLPDFHPPP
210 220 230 240 250
LPQEDPYCLD DAPPNLGYLV RMQGGVLFVY DNQTMLERQE PHSLPYPDLE
260 270 280 290 300
TYIVDMSHIL ALITDGPTKT YCHRRLNFLE SKFSLHEMLN EMSEFKELKS
310 320 330 340 350
NPHRDFYNVR KVDTHIHAAA CMNQKHLLRF IKYTYQTEPD RTVAEKLGRK
360 370 380 390 400
ITLRQVFDSL HMDPYDLTVD SLDVHAGRQT FHGFDKFNSK YNPVGASELR
410 420 430 440 450
DLYLKTENYL GGEYFARMVK EVARELEDSK YQYSEPRLSI YGRSPKEWSS
460 470 480 490 500
LARWFIQHKV YSPNMRWIIQ VPRIYDIFRS KKLLPSFGKM LENIFLPLFQ
510 520 530 540 550
ATINPQDHRE LHLFLKYVTG FDSVDDESKH SDHMFSDKSP SPDLWTSEQN
560 570 580 590 600
PPYSYYLYYM YANIMVLNNL RRERGLSTFL FRPHCGEAGS ITHLVSAFLT
610 620 630 640 650
ADNISHGLLL KKSPVLQYLY YLAQIPIAMS PLSNNSLFLE YSKNPLREFL
660 670 680 690 700
HKGLHVSLST DDPMQFHYTK EALMEEYAIA AQVWKLSTCD LCEIARNSVL
710 720 730 740 750
QSGLSHQEKQ KFLGQNYYKE GPEGNDIRKT NVAQIRMAFR YETLCNELSF
760
LSDAMKSEEI TALAD
Length:765
Mass (Da):88,483
Last modified:November 1, 1997 - v2
Checksum:iF826FA5966221B09
GO

Sequence cautioni

The sequence AAC53348 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90888 mRNA Translation: AAC53348.1 Different initiation.
RefSeqiNP_113732.1, NM_031544.1
UniGeneiRn.11106

Genome annotation databases

GeneIDi25095
KEGGirno:25095
UCSCiRGD:2111 rat [O09178-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAMPD3_RAT
AccessioniPrimary (citable) accession number: O09178
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health