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Protein

AMP deaminase 3

Gene

Ampd3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

AMP deaminase plays a critical role in energy metabolism.

Catalytic activityi

AMP + H2O = IMP + NH3.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from AMP.
Proteins known to be involved in this subpathway in this organism are:
  1. AMP deaminase 1 (Ampd1), AMP deaminase 2 (Ampd2), AMP deaminase 3 (Ampd3)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from AMP, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi315Zinc; catalyticBy similarity1
Metal bindingi317Zinc; catalyticBy similarity1
Binding sitei317SubstrateBy similarity1
Metal bindingi584Zinc; catalyticBy similarity1
Binding sitei587SubstrateBy similarity1
Active sitei606Proton acceptorPROSITE-ProRule annotation1
Metal bindingi661Zinc; catalyticBy similarity1

GO - Molecular functioni

  • AMP deaminase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • AMP catabolic process Source: RGD
  • IMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00591; UER00663.

Names & Taxonomyi

Protein namesi
Recommended name:
AMP deaminase 3 (EC:3.5.4.6)
Alternative name(s):
AMP deaminase isoform E
Gene namesi
Name:Ampd3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2111. Ampd3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001944121 – 765AMP deaminase 3Add BLAST765

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei85PhosphoserineBy similarity1
Modified residuei106PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO09178.
PRIDEiO09178.

PTM databases

iPTMnetiO09178.
PhosphoSitePlusiO09178.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024933.

Chemistry databases

BindingDBiO09178.

Structurei

3D structure databases

ProteinModelPortaliO09178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni386 – 391Substrate bindingBy similarity6
Regioni662 – 665Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1096. Eukaryota.
COG1816. LUCA.
HOGENOMiHOG000092200.
HOVERGENiHBG050494.
InParanoidiO09178.
KOiK01490.
PhylomeDBiO09178.

Family and domain databases

CDDicd01319. AMPD. 1 hit.
InterProiIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR006329. AMPD.
IPR029771. AMPD3.
IPR032466. Metal_Hydrolase.
[Graphical view]
PANTHERiPTHR11359. PTHR11359. 1 hit.
PTHR11359:SF2. PTHR11359:SF2. 1 hit.
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
PIRSFiPIRSF001251. AMP_deaminase_met. 1 hit.
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01429. AMP_deaminase. 1 hit.
PROSITEiPS00485. A_DEAMINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform 1 (identifier: O09178-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPRQFPKLNM SDLDEHVRLL AEKVFAKVLR EEDSKDVMSL FTVPKDCPIG
60 70 80 90 100
QKEAKERELQ KELAEQKSVE TAKRKKSFKM IRSQSMSLQM PTQDWKGPPS
110 120 130 140 150
VSPAMSPTTP LVLGAASKPG LAPYDMPEYQ RATISGDYCA GITMEDYEQA
160 170 180 190 200
AKSLAKALMI REKYARLAYH RFPRTTAQYL AHQGESVPLE EGLPDFHPPP
210 220 230 240 250
LPQEDPYCLD DAPPNLGYLV RMQGGVLFVY DNQTMLERQE PHSLPYPDLE
260 270 280 290 300
TYIVDMSHIL ALITDGPTKT YCHRRLNFLE SKFSLHEMLN EMSEFKELKS
310 320 330 340 350
NPHRDFYNVR KVDTHIHAAA CMNQKHLLRF IKYTYQTEPD RTVAEKLGRK
360 370 380 390 400
ITLRQVFDSL HMDPYDLTVD SLDVHAGRQT FHGFDKFNSK YNPVGASELR
410 420 430 440 450
DLYLKTENYL GGEYFARMVK EVARELEDSK YQYSEPRLSI YGRSPKEWSS
460 470 480 490 500
LARWFIQHKV YSPNMRWIIQ VPRIYDIFRS KKLLPSFGKM LENIFLPLFQ
510 520 530 540 550
ATINPQDHRE LHLFLKYVTG FDSVDDESKH SDHMFSDKSP SPDLWTSEQN
560 570 580 590 600
PPYSYYLYYM YANIMVLNNL RRERGLSTFL FRPHCGEAGS ITHLVSAFLT
610 620 630 640 650
ADNISHGLLL KKSPVLQYLY YLAQIPIAMS PLSNNSLFLE YSKNPLREFL
660 670 680 690 700
HKGLHVSLST DDPMQFHYTK EALMEEYAIA AQVWKLSTCD LCEIARNSVL
710 720 730 740 750
QSGLSHQEKQ KFLGQNYYKE GPEGNDIRKT NVAQIRMAFR YETLCNELSF
760
LSDAMKSEEI TALAD
Length:765
Mass (Da):88,483
Last modified:November 1, 1997 - v2
Checksum:iF826FA5966221B09
GO

Sequence cautioni

The sequence AAC53348 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90888 mRNA. Translation: AAC53348.1. Different initiation.
RefSeqiNP_113732.1. NM_031544.1.
UniGeneiRn.11106.

Genome annotation databases

GeneIDi25095.
KEGGirno:25095.
UCSCiRGD:2111. rat. [O09178-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90888 mRNA. Translation: AAC53348.1. Different initiation.
RefSeqiNP_113732.1. NM_031544.1.
UniGeneiRn.11106.

3D structure databases

ProteinModelPortaliO09178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024933.

Chemistry databases

BindingDBiO09178.

PTM databases

iPTMnetiO09178.
PhosphoSitePlusiO09178.

Proteomic databases

PaxDbiO09178.
PRIDEiO09178.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi25095.
KEGGirno:25095.
UCSCiRGD:2111. rat. [O09178-1]

Organism-specific databases

CTDi272.
RGDi2111. Ampd3.

Phylogenomic databases

eggNOGiKOG1096. Eukaryota.
COG1816. LUCA.
HOGENOMiHOG000092200.
HOVERGENiHBG050494.
InParanoidiO09178.
KOiK01490.
PhylomeDBiO09178.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00663.

Miscellaneous databases

PROiO09178.

Family and domain databases

CDDicd01319. AMPD. 1 hit.
InterProiIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR006329. AMPD.
IPR029771. AMPD3.
IPR032466. Metal_Hydrolase.
[Graphical view]
PANTHERiPTHR11359. PTHR11359. 1 hit.
PTHR11359:SF2. PTHR11359:SF2. 1 hit.
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
PIRSFiPIRSF001251. AMP_deaminase_met. 1 hit.
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01429. AMP_deaminase. 1 hit.
PROSITEiPS00485. A_DEAMINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPD3_RAT
AccessioniPrimary (citable) accession number: O09178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.