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Protein

Aminopeptidase B

Gene

Rnpep

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg0-Leu-enkephalin, Arg0-Met-enkephalin and Arg(-1)-Lys0-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4).

Catalytic activityi

Release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi325Zinc; catalytic1
Active sitei326Proton acceptorPROSITE-ProRule annotation1
Metal bindingi329Zinc; catalytic1
Metal bindingi348Zinc; catalytic1
Sitei414Transition state stabilizerBy similarity1

GO - Molecular functioni

  • aminopeptidase activity Source: UniProtKB
  • cobalt ion binding Source: RGD
  • copper ion binding Source: RGD
  • metalloaminopeptidase activity Source: RGD
  • peptide binding Source: RGD
  • zinc ion binding Source: RGD

GO - Biological processi

  • aging Source: RGD
  • negative regulation of blood pressure Source: RGD
  • peptide catabolic process Source: GO_Central
  • protein processing Source: UniProtKB
  • proteolysis Source: RGD
  • retina development in camera-type eye Source: RGD

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.4.11.6 5301

Protein family/group databases

MEROPSiM01.014

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase B (EC:3.4.11.6)
Short name:
AP-B
Alternative name(s):
Arginine aminopeptidase
Arginyl aminopeptidase
Cytosol aminopeptidase IV
Gene namesi
Name:Rnpep
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621137 Rnpep

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2452

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950901 – 650Aminopeptidase BAdd BLAST650

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphoserineBy similarity1
Modified residuei446N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO09175
PRIDEiO09175

2D gel databases

World-2DPAGEi0004:O09175

PTM databases

iPTMnetiO09175
PhosphoSitePlusiO09175

Expressioni

Tissue specificityi

Widely expressed.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009198

Chemistry databases

BindingDBiO09175

Structurei

3D structure databases

ProteinModelPortaliO09175
SMRiO09175
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni298 – 302Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiKOG1047 Eukaryota
COG0308 LUCA
HOGENOMiHOG000293296
HOVERGENiHBG001274
InParanoidiO09175
KOiK01260
PhylomeDBiO09175

Family and domain databases

CDDicd09599 M1_LTA4H, 1 hit
Gene3Di1.10.390.10, 1 hit
1.25.40.320, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR038502 M1_LTA-4_hydro/amino_C_sf
IPR034015 M1_LTA4H
IPR015571 Pept_M1_aminopeptidase-B
IPR001930 Peptidase_M1
IPR015211 Peptidase_M1_C
IPR014782 Peptidase_M1_N
IPR027268 Peptidase_M4/M1_CTD_sf
PANTHERiPTHR11533 PTHR11533, 1 hit
PTHR11533:SF153 PTHR11533:SF153, 1 hit
PfamiView protein in Pfam
PF09127 Leuk-A4-hydro_C, 1 hit
PF01433 Peptidase_M1, 1 hit
PRINTSiPR00756 ALADIPTASE
SMARTiView protein in SMART
SM01263 Leuk-A4-hydro_C, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

O09175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSGPSSCH SAARRPLHSA QAVDVASASS FRAFEILHLH LDLRAEFGPP
60 70 80 90 100
GPGPGSRGLN GKATLELRCL LPEGASELRL DSHSCLEVMA ATLLRGQPGD
110 120 130 140 150
QQQLTEPVPF HTQPFSHYGQ ALCVVFPKPC CAAERFRLEL TYRVGEGPGV
160 170 180 190 200
CWLAPEQTAG KKKPFVYTQG QAVLNRAFFP CFDTPAVKCT YSALVEVPDG
210 220 230 240 250
FTAVMSASTW ERRGPNKFFF QMSQPIPSYL IALAIGDLAS AEVGPRSRVW
260 270 280 290 300
AEPCLIEAAK EEYNGVIEEF LATGEKLFGP YVWGRYDLLF MPPSFPFGGM
310 320 330 340 350
ENPCLTFVTP CLLAGDRSLA DVIIHEISHS WFGNLVTNAN WGEFWLNEGF
360 370 380 390 400
TMYAQRRIST ILFGAAYTCL EAATGRALLR QHMDVSGEEN PLNKLRVKIE
410 420 430 440 450
PGVDPDDTYN ETPYEKGYCF VSYLAHLVGD QEQFDKFLKA YVDEFKFQSI
460 470 480 490 500
LAEDFLEFYL EYFPELKKKG VDSIPGFEFN RWLNTPGWPP YLPDLSPGDS
510 520 530 540 550
LMKPAEELAE LWAASEPDMQ AIEAVAISTW KTYQLVYFLD KILQKSPLPP
560 570 580 590 600
GNVKKLGETY PKISNAQNAE LRLRWGQIIL KNDHQEEFWK VKDFLQSQGK
610 620 630 640 650
QKYTLPLYHA MMGGSEMART LAKETFSATA SQLHSNVVNY VQQILAPKGS
Length:650
Mass (Da):72,620
Last modified:July 11, 2002 - v2
Checksum:i032BE2F4F50B58E0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47 – 48FG → L in AAB52971 (PubMed:9096329).Curated2
Sequence conflicti74Missing in BAA13413 (PubMed:8940051).Curated1
Sequence conflicti250 – 251WA → T in AAB52971 (PubMed:9096329).Curated2
Sequence conflicti627S → A in BAA13413 (PubMed:8940051).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61696 mRNA Translation: AAB52971.1
D87515 mRNA Translation: BAA13413.1
RefSeqiNP_112359.1, NM_031097.1
UniGeneiRn.10979

Genome annotation databases

GeneIDi81761
KEGGirno:81761
UCSCiRGD:621137 rat

Similar proteinsi

Entry informationi

Entry nameiAMPB_RAT
AccessioniPrimary (citable) accession number: O09175
Secondary accession number(s): P97530
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 11, 2002
Last modified: May 23, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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