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Protein

Glutamate--cysteine ligase regulatory subunit

Gene

Gclm

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase catalytic subunit (Gclc), Glutamate--cysteine ligase regulatory subunit (Gclm)
  2. Glutathione synthetase (Gss), Glutathione synthetase (Gss), Glutathione synthetase (Gss)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • apoptotic mitochondrial changes Source: MGI
  • cellular response to fibroblast growth factor stimulus Source: Ensembl
  • cellular response to follicle-stimulating hormone stimulus Source: Ensembl
  • cellular response to glucose stimulus Source: Ensembl
  • cellular response to hepatocyte growth factor stimulus Source: Ensembl
  • cellular response to thyroxine stimulus Source: Ensembl
  • cysteine metabolic process Source: MGI
  • glutamate metabolic process Source: UniProtKB
  • glutathione biosynthetic process Source: MGI
  • glutathione metabolic process Source: MGI
  • hepatic stellate cell activation Source: Ensembl
  • negative regulation of extrinsic apoptotic signaling pathway Source: MGI
  • negative regulation of neuron apoptotic process Source: Ensembl
  • positive regulation of glutamate-cysteine ligase activity Source: MGI
  • regulation of blood vessel size Source: UniProtKB
  • regulation of mitochondrial depolarization Source: MGI
  • response to activity Source: Ensembl
  • response to drug Source: UniProtKB
  • response to human chorionic gonadotropin Source: Ensembl
  • response to nitrosative stress Source: Ensembl
  • response to nutrient Source: Ensembl
  • response to oxidative stress Source: MGI

Keywordsi

Biological processGlutathione biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-174403. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00209.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--cysteine ligase regulatory subunit
Alternative name(s):
GCS light chain
Gamma-ECS regulatory subunit
Gamma-glutamylcysteine synthetase regulatory subunit
Glutamate--cysteine ligase modifier subunit
Gene namesi
Name:Gclm
Synonyms:Glclr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:104995. Gclm.

Subcellular locationi

GO - Cellular componenti

  • glutamate-cysteine ligase complex Source: MGI

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001925741 – 274Glutamate--cysteine ligase regulatory subunitAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei59PhosphoserineCombined sources1
Modified residuei263N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO09172.
PaxDbiO09172.
PeptideAtlasiO09172.
PRIDEiO09172.

PTM databases

iPTMnetiO09172.
PhosphoSitePlusiO09172.
SwissPalmiO09172.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028124.
CleanExiMM_GCLM.
ExpressionAtlasiO09172. baseline and differential.
GenevisibleiO09172. MM.

Interactioni

Subunit structurei

Heterodimer of a catalytic heavy chain and a regulatory light chain.

GO - Molecular functioni

  • glutamate-cysteine ligase catalytic subunit binding Source: MGI
  • protein heterodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi199941. 1 interactor.
IntActiO09172. 1 interactor.
MINTiMINT-1854716.
STRINGi10090.ENSMUSP00000029769.

Structurei

3D structure databases

ProteinModelPortaliO09172.
SMRiO09172.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3023. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00510000047658.
HOGENOMiHOG000007111.
HOVERGENiHBG005923.
InParanoidiO09172.
KOiK11205.
OMAiEYLQPYW.
OrthoDBiEOG091G0DYU.
PhylomeDBiO09172.
TreeFamiTF105986.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiView protein in InterPro
IPR032963. Gclm.
IPR023210. NADP_OxRdtase_dom.
PANTHERiPTHR13295. PTHR13295. 1 hit.
PfamiView protein in Pfam
PF00248. Aldo_ket_red. 1 hit.
SUPFAMiSSF51430. SSF51430. 1 hit.

Sequencei

Sequence statusi: Complete.

O09172-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTDSRAAGA LLARASTLHL QTGNLLNWGR LRKKCPSTHS EELRDCIQKT
60 70 80 90 100
LNEWSSQISP DLVREFPDVL ECTMSHAVEK INPDEREEMK VSAKLFIVGS
110 120 130 140 150
NSSSSTRSAV DMACSVLGVA QLDSVIMASP PIEDGVNLSL EHLQPYWEEL
160 170 180 190 200
ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL
210 220 230 240 250
TAFAKQFDIQ LLTHNDPKEL LSEASFQEAL QESIPDIEAQ DWVPLWLLRY
260 270
SVIVKSRGII KSKGYILQAK RRGS
Length:274
Mass (Da):30,535
Last modified:July 1, 1997 - v1
Checksum:iE1D591CAB71CEA11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U95053 mRNA. Translation: AAB96893.1.
AF149060
, AF149054, AF149055, AF149056, AF149057, AF149058, AF149059 Genomic DNA. Translation: AAG15424.1.
AK028236 mRNA. Translation: BAC25831.1.
CCDSiCCDS17808.1.
RefSeqiNP_032155.1. NM_008129.4.
UniGeneiMm.292676.

Genome annotation databases

EnsembliENSMUST00000029769; ENSMUSP00000029769; ENSMUSG00000028124.
ENSMUST00000178826; ENSMUSP00000136987; ENSMUSG00000028124.
GeneIDi14630.
KEGGimmu:14630.
UCSCiuc008reo.2. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGSH0_MOUSE
AccessioniPrimary (citable) accession number: O09172
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: July 1, 1997
Last modified: June 7, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families