Reviewed, UniProtKB/Swiss-Prot O09171 (BHMT1_RAT)

Names and origin

Protein names

Recommended name:
    Betaine--homocysteine S-methyltransferase 1
    EC=2.1.1.5

Gene names

Name:

Bhmt

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Catalytic activity	Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine.
Cofactor	Binds 1 zinc ion per subunit.
Pathway	Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
Subunit structure	Homotetramer. Ref.4
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 Hcy-binding domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from direct assay. Source: RGD protein amino acid methylation Inferred from direct assay. Source: RGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell protein complex Inferred from direct assay. Source: RGD
Molecular function	betaine-homocysteine S-methyltransferase activity Inferred from direct assay. Source: RGD homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro protein complex binding Inferred from direct assay. Source: RGD zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 407

407

Betaine--homocysteine S-methyltransferase 1

PRO_0000114624

Regions

Domain

11 – 314

304

Hcy-binding

Sites

Metal binding

217

1

Zinc

Metal binding

299

1

Zinc

Metal binding

300

1

Zinc

Experimental info

Sequence conflict

247 – 248

2

HA → QP in AAH78811. Ref.3

Secondary structure

1

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407

Helix Strand Turn

Details...

Helix

13 – 18

6

Helix

29 – 35

7

Turn

41 – 43

3

Helix

48 – 51

4

Helix

53 – 66

14

Beta strand

70 – 72

3

Helix

96 – 112

17

Turn

113 – 115

3

Beta strand

117 – 122

6

Helix

126 – 129

4

Helix

134 – 150

17

Beta strand

154 – 158

5

Helix

164 – 175

12

Beta strand

181 – 185

5

Helix

198 – 207

10

Beta strand

211 – 219

9

Helix

221 – 236

16

Turn

237 – 239

3

Beta strand

243 – 247

5

Helix

261 – 263

3

Turn

265 – 269

5

Helix

272 – 274

3

Helix

278 – 291

14

Helix

304 – 313

10

Helix

315 – 318

4

Helix

323 – 327

5

Beta strand

330 – 332

3

Helix

333 – 335

3

Helix

341 – 344

4

Helix

349 – 354

6

Helix

378 – 397

20

Sequences

	Sequence		Length	Mass (Da)
	O09171-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 36E1D04A8E425887	FASTA	407	44,976
	10 20 30 40 50 60 MAPIAGKKAK RGILERLNAG EVVIGDGGFV FALEKRGYVK AGPWTPEAAV EHPEAVRQLH 70 80 90 100 110 120 REFLRAGSNV MQTFTFYASE DKLENRGNYV AEKISGQKVN EAACDIARQV ADEGDALVAG 130 140 150 160 170 180 GVSQTPSYLS CKSETEVKKI FHQQLEVFMK KNVDFLIAEY FEHVEEAVWA VEALKTSGKP 190 200 210 220 230 240 IAATMCIGPE GDLHGVSPGE CAVRLVKAGA AIVGVNCHFD PSTSLQTIKL MKEGLEAARL 250 260 270 280 290 300 KAYLMSHALA YHTPDCGKQG FIDLPEFPFG LEPRVATRWD IQKYAREAYN LGVRYIGGCC 310 320 330 340 350 360 GFEPYHIRAI AEELAPERGF LPPASEKHGS WGSGLDMHTK PWIRARARKE YWQNLRIASG 370 380 390 400 RPYNPSMSKP DAWGVTKGAA ELMQQKEATT EQQLRALFEK QKFKSAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Application of mRNA differential display to liver cirrhosis: reduced fetuin expression in biliary cirrhosis in the rat." Forestier M., Reichen J., Solioz M. Biochem. Biophys. Res. Commun. 225:377-383(1996) [PubMed: 8753772] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Apolipoprotein B mRNA and lipoprotein secretion are increased in McArdle RH-7777 cells by expression of betaine-homocysteine S-methyltransferase." Sowden M.P., Collins H.L., Smith H.C., Garrow T.A., Sparks J.D., Sparks C.E. Biochem. J. 341:639-645(1999) [PubMed: 10417327] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[4]	"Crystal structure of rat liver betaine homocysteine S-methyltransferase reveals new oligomerization features and conformational changes upon substrate binding." Gonzalez B., Pajares M.A., Martinez-Ripoll M., Blundell T.L., Sanz-Aparicio J. J. Mol. Biol. 338:771-782(2004) [PubMed: 15099744] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U96133 mRNA. Translation: AAB53763.1.
AF038870 mRNA. Translation: AAB95481.1.
BC078811 mRNA. Translation: AAH78811.1.

IPI

IPI00332027.

RefSeq

NP_110477.1.

UniGene

Rn.11406

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UMY	X-ray	2.50	A/B/C/D	1-407	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

O09171.

Proteomic databases

PRIDE

O09171.

Genome annotation databases

Ensembl

ENSRNOT00000015336; ENSRNOP00000015336; ENSRNOG00000011200; Rattus norvegicus. [Genome view]

GeneID

81508.

KEGG

rno:81508.

UCSC

NM_030850. rat.

Organism-specific databases

CTD

81508.

RGD

621496. Bhmt.

Phylogenomic databases

HOVERGEN

O09171.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-9241.

BRENDA

2.1.1.5. 248.

Gene expression databases

ArrayExpress

O09171.

Genevestigator

O09171.

GermOnline

ENSRNOG00000011200. Rattus norvegicus.

Family and domain databases

InterPro

IPR017226. Betaine-hCys_S-MeTrfase_BHMT.
IPR003726. S_MeTrfase.
[Graphical view]

Gene3D

G3DSA:3.20.20.330. S_methyl_trans. 1 hit.

Pfam

PF02574. S-methyl_trans. 1 hit.
[Graphical view]

PIRSF

PIRSF037505. Betaine_HMT. 1 hit.

PROSITE

PS50970. HCY. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

614975.

Entry information

Entry name

BHMT1_RAT

Accession

Primary (citable) accession number: O09171
Secondary accession number(s): Q6AZ06

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	October 13, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)